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Biomedical subjects

I Bülthoff

Publications and source records attributed to I Bülthoff.

3 recordsLinked to original sources

GABA-antagonist inverts movement and object detection in flies.

Movement detection is one of the most elementary visual computations performed by vertebrates as well as invertebrates. However, comparatively little is known about the biophysical mechanisms underlying this computation. It has been proposed on both physiological and theoretical grounds that inhibition plays a crucial role in the directional selectivity of elementary movement detectors (EMDs). For the first time, we have studied electrophysiological and behavioral changes induced in flies after application of picrotoxinin, an antagonist of GABA. The results show that inhibitory interactions play an important role in movement detection in flies. Furthermore, our behavioral results suggest that the computation of object position is based primarily on movement detection.

Action Potentials

Freeze-substitution of Drosophila heads for subsequent [3H]2-deoxyglucose autoradiography.

High resolution of [3H]2-deoxyglucose labelling was obtained in autoradiographs of Drosophila brains after freeze-substitution in anhydrous acetone at -76 degrees C. This method was applied to preparations which received visual, olfactory and mechanosensory stimulation. The autoradiographs were compared to those obtained after freeze-drying. Freeze-substitution, which has proved to be technically simple, rapid and inexpensive, yields a good quality of tissue preservation and hence is recommended for tissue dehydration prior to autoradiography.

Animals