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Biomedical subjects

I Bradvarova

Publications and source records attributed to I Bradvarova.

7 recordsLinked to original sources
PubMed1987

[In vitro research on the interaction of natural zeolites with diploid cells from the human embryonic lung].

A study of the biological effect of the natural zeolites, clinoptilolite, mordenite and erionite on monostratum cellular culture of human embryonic lung is performed. The number of pyknotic cells, morphological changes in the cells, and monostratum, mitotic activity and changes in the cellular culture are reported during a continuous experiment. As positive control is used standard quartz dust DQ-12 and for negative dust of TiO2. It is already established that the dust particles of natural zeolites with dimensions under 5 micron display biological aggressiveness. With most strongly expressed effect is the clinoptilolite dust, which related to identical active surface, is comparable to that of quartz DQ-12. The mordenite is with moderate cytotoxic effect, proportional to the time and concentration, while erionite is with slight and very delayed effect. There are differences concerning the effect on the miotic activity of the cells. The biologic aggressiveness of the zeolites is discussed in relation to their strong active surface, their ionometabolic properties and their capacity to generate hydrogen at moistering.

Aluminum Silicates↗
PubMed1986

Induction of chromosomal aberrations in human cells by a temperature-sensitive mutant of herpes simplex virus type 2 and its revertants.

The induction of chromosomal aberrations by a temperature-sensitive (ts) mutant of herpes simplex virus type 2 (HSV-2) strain Hg52 (ts 13), its revertants 4-8 and 5-8 and by etalon strains HSV-1 17 syn+ and HSV-2 Hg52 was studied in human fibroblast and lymphocyte cultures. The effect on chromosomes of the revertants was tested at permissive (31 degrees C) and non-permissive (38 degrees C) temperatures. At 38 degrees C the revertants could not induce DNase activity. The present results contribute to the possible role of a herpes-coded nuclease in induction of chromosomal aberrations.

Cells, Cultured↗
PubMed1984

Effects of herpes simplex virus strains on human fibroblast and lymphocyte chromosomes and the localization of chromosomal aberrations.

The effects on human chromosomes of types 1, 2 and of intermediate serotype of herpes simplex virus (HSV) was compared in fibroblast and lymphocyte cultures. The karyological changes due to HSV were shown to depend on the serotype used as well as on the kind of cells examined (agent-specificity and cell-reaction specificity). Differences were noted among the strains in relation to the degree and character of the aberrations induced. Conventional Giemsa staining and the trypsin G-banding techniques were used to localize aberrations in the length of human fibroblast and lymphocyte chromosomes after HSV infection. A non-random damage of chromosomes 1 and 3 displaying the same pattern in either cell type was established. The distribution of chromosomal abnormalities was independent of the chromosome length. The topographic banding analysis of lesions induced by strains of HSV-1, HSV-2 and intermediate serotypes showed that the most frequent aberrations were localized in bands p32, p34, q21 and q32 of chromosome 1 and in the band q21 of chromosome 3. The localization of the most frequently occurring aberrations in the chromosomes belonging to other groups was also determined.

Cells, Cultured↗