STIMULATION OF GASTRIC INTRINSIC FACTOR SECRETION.
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Biomedical subjects
Publications and source records attributed to I CHANARIN.
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Methods of preparing liver for the microbiological assay of folic-acid activity have been compared. Maximum values were obtained by incubating an alkaline homogenate of liver in the presence of ascorbic acid for five hours at 37 degrees C. Most other procedures resulted in significant loss of folic-acid activity.
Measurement of the serum Lactobacillus casei (;folic-acid') activity is widely used as an index of folic-acid deficiency. Present methods of assay result in recovery of about half the active material in normal serum. A modified method of assay is described which gives higher L. casei values and a clearer distinction between the sera of normal subjects and of patients with folic-acid deficiency.
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