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Biomedical subjects

I Cameron

Publications and source records attributed to I Cameron.

42 records · Page 3Linked to original sources
PubMed1979

Total parenteral nutrition and inhibition of gluconeogenesis on tumor-host responses.

Rats bearing the Morris hepatoma No. 7777 were randomized into three treatment groups. Two of the groups received a nutritionally complete liquid formula diet per os ad libitum. One of these two groups received hydrazine sulfate (HS; an inhibitor of gluconeogenesis) twice daily (15 mg/kg) for 5 days. A third group of tumorous rats received the HS therapy and was given the liquid diet parenterally for 5 days. Tumorous rats fed per os, especially with HS therapy demonstrated inhibition of tumor growth, reduction of body and carcass weight, anorexia and decreased nitrogen retention. The combination of parenteral feeding and HS therapy sustained body and carcass weight with high nitrogen retention but stimulated tumor growth and was associated with liver toxicity. These results support the concept that cancer cachexia involves 'a systemic energy-losing cycle dependent on an interplay of tumor glycolysis and gluconeogenesis'.

Animals↗
PubMedDate not supplied

Physiological levels of hydrostatic pressure alter morphology and organization of cytoskeletal and adhesion proteins in MG-63 osteosarcoma cells.

The response of human MG-63 osteosarcoma cells to physiological levels of hydrostatic pressure was studied. Cell cultures were subjected to a 20-min, 4-MPa hydrostatic pressure pulse. Adhesion was measured at 20 min and 2 h post-hydrostatic pressure. Morphometric measurements of cell shape and immunofluorescent assays of cytoskeletal and adhesion proteins were done pre- and post-hydrostatic pressure. Pressure-treated cells showed increased adhesion (resistance to deadhesion by trypsinization)-with increased recovery time. Indirect immunofluorescence demonstrated increased heterotypic adhesion receptor at cell-cell interfaces and increased alpha 3, beta 1-integrin at cell-substrate interfaces. Indirect immunofluorescence demonstrated depolymerization of alpha-tubulin, vimentin, and actin during the pressure pulse. Actin reorganization was slower than that of alpha-tubulin and vimentin, with stress filaments not well organized even after 1 h postpressure. The depolymerization of alpha-tubulin, vimentin, and actin observed at relatively low levels of hydrostatic pressure suggests disintegration of the integrin-cytoskeletal attachment complex. The increased resistance of the cells to trypsinization and the increase in both heterotypic adhesion receptor and the alpha 3, beta 1-integrin at cell interfaces suggest that cells compensate for loss of cytoskeletal integrity by increasing attachment to both adjacent cells and the extracellular matrix.

Bone and Bones↗
PubMedDate not supplied

Carer burden after proximal femoral fracture.

This carer study forms part of a wider study which compares accelerated rehabilitation after proximal femoral fracture with conventional care. Two measures of burden on family caregivers were used; social worker's assessment of overall burden and an index of disruption to carers' lives. Participants were carers of 200 patients interviewed at five periods up to 12 months after fracture. Carer burden prior to the fracture was the strongest predictor of subsequent burden, with carers of patients in nursing homes and patients with a cognitive or physical disability being more burdened. Carer burden may increase or decrease after the patient's injury, irrespective of randomization group. There is no simple relationship between burden and randomized group. Accelerated rehabilitation does not impact greatly on carer burden, but already severely burdened carers may benefit from additional counselling/information. Specific ways in which carers; lives were disrupted are reported.

Aged↗