PubMed Health⌕ Search

Biomedical subjects

I Crawford

Publications and source records attributed to I Crawford.

9 recordsLinked to original sources
PubMed1992

CFTR protein expression in primary and cultured epithelia.

The gene responsible for the lethal disorder cystic fibrosis encodes a 1480-amino acid glycoprotein, CFTR. Using polyclonal antibodies directed against separate phosphorylation sites in the pre-nucleotide-binding fold (exon 9) and the R domain (exon 13), we have identified a 165-kDa protein in Xenopus laevis oocytes injected with recombinant CFTR cRNA transcribed from the full-length CFTR plasmid pBQ4.7. A protein of the same mobility was also detected with Western blotting techniques in whole cell extracts of cells that express CFTR mRNA (T84, FHTE, HT-29), including biopsied human nasal and bronchial tissue. Immunodetectable 165-kDa protein was concentrated in the apical membrane fraction of ileal villus tissue. We also report that the 165-kDa protein levels can be modulated pharmacologically, and these levels are appropriately correlated with second-messenger-regulated Cl- efflux. Thus, native or recombinant CFTR can be recognized by these anti-CFTR peptide polyclonal antibodies.

Animals↗
PubMed1991

Immunocytochemical localization of the cystic fibrosis gene product CFTR.

Antisera against two peptides, corresponding to different domains of the cystic fibrosis gene product CFTR, have been raised and extensively characterized. Both antisera recognize CFTR as a 165-kDa polypeptide in Western analysis of cells transfected with CFTR cDNA as well as in epithelial cell lines. The cell and tissue distribution of CFTR has been studied by immunocytochemistry. CFTR is abundant in epithelial cells, including those lining sweat ducts, small pancreatic ducts, and intestinal crypts. Unexpectedly, the level of CFTR in lung epithelia is relatively low, while it is abundant in the epithelia of kidney tubules. The protein appears to be restricted to the apical, rather than basolateral, regions of epithelial cells and at least a proportion is associated with the plasma membrane. The cell and tissue distributions of CFTR are consistent with a function for this protein as a chloride channel or as a regulator of channel activity.

Animals↗
PubMed1990

Attitudes of undergraduate college students toward AIDS.

This study examined attitudes about AIDS and the effects of those attitudes on the behavior of 131 undergraduate college students. Participants reported having minimal concern about contracting AIDS from their current or future sexual partners, and their rate of sexual activity had not changed from that of the previous year. Of all participants 69% reported they did not use condoms during their sexual encounters. White students were more knowledgeable about AIDS than their peers from minority groups. Although all participants were aware they could not contract the AIDS virus through common social interaction, 80% reported they would feel very anxious if a classmate who had AIDS continued to attend classes. These findings suggest that these undergraduate students underestimate their risk of HIV infection and so jeopardize their health.

Acquired Immunodeficiency Syndrome↗
PubMed1986

Comparative adhesion of seven species of streptococci isolated from the blood of patients with sub-acute bacterial endocarditis to fibrin-platelet clots in vitro.

The adhesion to fibrin-platelet clots in vitro of 21 strains of streptococci isolated from the blood of patients with sub-acute bacterial endocarditis (SABE) was measured. The species, in order of greatest adhesion, were Streptococcus faecalis, Streptococcus mutans, Streptococcus milleri, Streptococcus sanguis, dextran-positive Streptococcus mitior, dextran-negative Streptococcus mitior and Streptococcus salivarius. Individual strains within species, however, cannot be assumed to be representative of their species and may exhibit unusually high or low adhesion. Adhesion depended upon both bacterial concentration and period of contact. There was no simple relationship between ability to adhere and liability to cause endocarditis. Formation of dextran did not increase adhesion. The streptococci were more adhesive than strains of Escherichia coli and Neisseria sicca and less adhesive than strains of Staphylococcus aureus and Streptococcus pyogenes.

Adhesiveness↗
PubMed1983

Streptococci isolated from the bloodstream and gingival crevice of man.

One hundred and twenty six strains of streptococci isolated from the bloodstreams of 123 hospital in-patients--55 with endocarditis--and 255 strains isolated from the gingival crevices of 66 volunteers were characterised. Species isolated were Streptococcus mitior, S. sanguis, undifferentiated viridans streptococci, S. salivarius, S. milleri, S. mutans, S. bovis and S. faecalis. There was no significant difference between the distribution of species in blood and in the gingival crevice. S. mitior and S. sanguis were found less frequently in blood than their presence in the gingival crevice would have suggested, whereas S. milleri was found more frequently. This may indicate that S. milleri is more invasive or that it enters the bloodstream from sources other than the gingival crevice.

Endocarditis, Subacute Bacterial↗