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Biomedical subjects

I Crepinko

Publications and source records attributed to I Crepinko.

At least 19 recordsLinked to original sources
PubMed1986

Cytomorphological and cytochemical classification of acute myeloid leukaemia (AML).

One hundred cases of adult AML were classified cytomorphologically (FAB-classification) and cytochemically (Loeffler's classification). From this combined analysis it could be shown that some corrections have to be made in the FAB-classification; particularly groups M-1 and M-4 had to be reclassified. The authors, therefore, advocate that the final FAB-classification should be the result of cytomorphologic as well as cytochemical analysis.

Female↗
PubMed1981

Cytological and biochemical methods for the characterization of in vitro cultured cells.

The aim of our presentation was to show how we characterize cells cultured in monolayer system. Cytological and biochemical methods were used. Ovarian Krukenberg tumour fibroblasts were investigated and findings were correlated with normal human diploids (HDZ1) and with fibroblasts obtained from Blighted ovum. Cytomorphologically Malignancy associated changes in the tumour fibroblasts were found. Cytochemically acid phosphatase and alpha-naphtyl-esterase were positive (+++). PAS reaction was doubled in 18th passage. Cytogenetically normal human diploids were found. Biochemically enzymatic assay showed phosphopentose shunt is decreased in tumour fibroblasts and alpha-glucosidase and beta-galactosidase activities were significantly lower in these cells. A form of N-acetyl-beta-glucosaminidase fell during the investigation from normal 75% to lower percent (42% of the total activity). Much more parameters were obtained by different methods and Krukenberg tumour fibroblasts may be better understood. In vitro investigation makes a contribution to biomedical knowledge in cancer research.

Enzymes↗