PubMed Health⌕ Search

Biomedical subjects

I Cuesta

Publications and source records attributed to I Cuesta.

7 recordsLinked to original sources
PubMed2000

Comparative study of two techniques for assessing fat intake in a Mediterranean diet. The influence of culinary oils changes.

The diet usually consumed by a community of 18 normolipemic monks was studied. It included olive oil as cooking fat (basal period BP). This diet was modified by substituting olive oil for sunflower oil during a 21-day period (experimental period EP). The precise individual weighing technique was utilised for the determination of intake, and by means of the food composition tables (FCTs) the energy and nutrient of the diet were calculated. By chemical analysis of the duplicate sample (CA) of the cooked dishes the lipid intake was also determined. Total serum cholesterol HDL and LDL cholesterol, serum triglycerides, AI and B-100 apolipoproteins and Lp(a) were also analysed. Similarly the absolute and relative values for SFA, MUFA and PUFA obtained by both techniques were also different and these were maintained when the (PUFA + MUFA)/SFA ratio was calculated by both techniques and in the two periods. The correction of the lipid intake by means of the CA technique did not improve either the correlation between the intake or the plasma lipid concentration or the cholesterolemia prediction (r = 0.83 for FCT and CA).

Adult↗
PubMed2000

Structural phosphoprotein M2-1 of the human respiratory syncytial virus is an RNA binding protein.

The structural phosphoprotein M2-1 of human respiratory syncytial virus (HRSV) Long strain shows RNA binding capacity in three different assays that detect RNA-protein complexes: cross-linking, gel retardation, and Northern-Western assays. It is able to bind HRSV leader RNA specifically with cooperative kinetics, with an apparent K(d) of at least 90 nM. It also binds to long RNAs with no sequence specificity. The RNA binding domain has been located between amino acid residues 59 and 85, at the NH(2) terminus of the protein. This region contains the phosphorylatable amino acid residues threonine 56 and serine 58, whose modification decreases the binding capacity of M2-1 protein to long RNAs.

5' Untranslated Regions↗
PubMed1998

[Infectious endocarditis caused by Leuconostoc mesenteroides].

BACKGROUND: Leuconostoc spp. are gram-positive coccobacilli, catalase and oxidase negative, vancomycin resistant, with a not clearly defined role in human infectious. Cases of infection have been reported previously but it has not been described confirmed infective endocarditis due to Leuconostoc mesenteroides. METHODS: We describe a case of prosthetic valve endocarditis in a 72-years-old woman with a long previous history of cardiac disease but without other immunological disorders. She developed a heart failure because of prosthetic aortic valve dysfunction. CONCLUSIONS: Leuconostoc mesenteroides should be considered as a potential cause of infective endocarditis and listed in the group of vancomycin-resistant microorganism.

Aged↗
PubMed1994

[Retrospective analysis of hemotherapy support in 226 cases of myelodysplastic syndromes].

PURPOSE: To evaluate the transfusion requirements (red cells and platelets) in 226 patients with myelodysplastic syndromes (MDS). PATIENTS AND METHODS: The number of patients under study was 226: 59 with refractory anaemia (RA), 49 with sideroblastic anaemia (RSA), 56 with refractory anaemia with excess of blast cells (RAEB), 48 with RAEB in transformation (RAEBT), and 14 with chronic myelomonocytic leukaemia (CMML). The period of the study was from January 1975 to December 1992. The mean age of the series was 67.4 years (ranging between 28 and 92) and the male/female ratio was 1.8. Transfusion frequency (TF) was defined as the time elapsed between two consecutive transfusions of two units of packed red cells. A platelet transfusion (PT) was comprised of 6 units of platelets or 1 unit obtained by thrombopheresis. The statistical analysis was performed with the chi 2 and Student's tests. RESULTS: Anaemia was present in 96.9% of the patients, regardless of the cytologic classification. Haemorrhages appeared in 41.6% of the cases with maximal frequency in CMML (78.6%) and minimal in RSA (10.2%). TF: Transfusion was required by 90.2% of the patients (86% of the RA, 79.6% of the RSA, 48.4% of RAEB, 97.9% of the RAEB-T, and 100% of the CMML). The mean TF was 1.5 months (1.9 for RA, 1.7 for RSA, 1.3 for RAEB, 0.9 for RAEB-T, 2.2. for CMML). PT: Platelet support was needed by 38.1% of the patients (30.5% of RA, 8.1% of RSA, 48.2% of RAEB, 58.3% of RAEB-T and 64.3% of CMML). The mean number of PT per patient was 7.2 (5.2 for RA, 3.8 for RSA, 9.7 for RAEB, 10.2 for RAEB-T, 7.1 for CMML). STATISTICAL ANALYSIS: The differences appreciated in TF were significant for the good prognostic groups (RA + RSA) as compared with the poor-risk ones (AREB + AREB-T + CMML) (p < 0.01), although significance is lost when these groups were taken one by one. For PT, significant differences appeared when comparing RA + RSA with RAEB + RAEB-T + CMML (p < 0.001); when the groups were compared one by one, significant differences were found only for RA versus RSA (p < 0.01). CONCLUSIONS: 1) Red cell support was required by 90.2% of the patients with MDS, while platelet support was needed in 38.1% of the cases. 2) The cytologic poor-risk groups (RAEB, RAEB-T and CMML) required greater transfusion support as a whole than did the good prognostic ones (RA + RSA). 3) However, only RA showed significant differences with respect to RSA regarding PT, the remaining groups showing no such differences. 4) An irregular pattern was seen in RA and CMML with respect to PT in the former and to both TF and PT in the latter.

Adult↗