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Biomedical subjects

I D Gust

Publications and source records attributed to I D Gust.

At least 163 records · Page 9Linked to original sources
PubMed1976

Detection of hepatitis b surface antigen in patients with acute viral hepatitis.

The relative value of solid phase radioimmunoassay and counter immunoelectrophoresis for the detection of hepatitis B surface antigen in the serum, was studied in 1401 patients with acute viral hepatitis. When specimens were obtained early in the illness, counter immunoelectrophoresis was found to be a reliable test for the diagnosis of hepatitis B, detecting 95.6% of patients found to be antigen positive by radioimmunoassay. The additional sensitivity of radioimmunoassay was of particular value for detecting antigen later in the illness and in patients with post-transfusion hepatitis in which the titre was frequently below the level detectable by counter immunoelectrophoresis. Radioimmunoassay was also of value in testing sera which had been stored for many years, detecting up to 3-3 times as many patients with hepatitis B as did counter immunoelectrophoresis.

Acute Disease↗
PubMed1976

A prospective study of acute viral hepatitis with particular reference to hepatitis A.

In order to investigate the relationship of hepatitis A antigen to viral hepatitis, a prospective study was carried out on 97 patients admitted to Fairfield Hospital, Melbourne, with suspected viral hepatitis, and 3 of their family contacts. Evidence of infection with hepatitis A virus was obtained by detecting hepatitis A antigen in stools, and/or antibody to it in sera, by immune electron microscopy. Infection with hepatitis B virus was determined by testing for hepatitis B surface antigen and antibody in serum, by solid phase radioimmunoassay. Sixteen patients were found to have diseases other than viral hepatitis and 2 patients (child contacts) suffered no illness. There was clinical and/or biochemical evidence compatible with viral hepatitis in 82 patients, of whom 35 were confirmed as having hepatitis A and 31 as having hepatitis B infections. In the remaining 16 patients there was no evidence of infection with either hepatitis A or B virus. It is possible that some of these patients may have been infected with viral agents as yet unidentified.

Acute Disease↗
PubMed1975

The detection of hepatitis B surface antigen by radioimmunoassay.

A comparative study was performed to assess the sensitivity and specificity of counterimmunoelectrophoresis (CIEP) and radioimmunoassay (RIA) for the detection of hepatitis B surface antigen. The 8,823 sera examined included selected reference panels and sera collected from populations with low, moderate and high rates of chronic antigen carriage. Overall, hepatitis B surface antigen was detected in 265 sera by CIEP and in 376 by RIA. As well as detecting 46.4% additional positives, the RIA test detected all CIEP-positive sera; i.e., there were no false negative results. However, 150 sera (1.8% of the total tested) gave a positive result by RIA which was not repeatable on retesting. The explanation for this phenomenon appeared to lie in inadequate washing of the antibody-coated tubes.

Blood Donors↗
PubMed1974

Enterovirus type 71 infection in Melbourne.

Between November 1972 and May 1973, 60 strains of a new enterovirus were isolated from 49 patients investigated at Fairfield Hospital for Communicable Diseases, Melbourne. Of these patients 39 were admitted to hospital with aseptic meningitis (which was accompanied by a rash in 6), 5 others had rash alone, 4 had acute respiratory tract infections, and 1 had infective polyneuritis. A representative strain from this outbreak had the physicochemical properties of an enterovirus but could not be identified with antisera prepared against the prototype polio, coxsackie, and echo viruses. Studies, performed in association with the WHO Collaborating Centre for Virus Reference and Research, Houston, TX, USA, showed the outbreak to be due to enterovirus 71. Most of the epidemic strains required sodium deoxycholate treatment before neutralization could be demonstrated.

Adolescent↗
PubMed1973

On the aetiology of whooping cough.

The results of routine bacterial and viral studies on 483 patients with whooping cough, investigated at one hospital over a five year period are presented. The possible role of respiratory viruses in the aetiology of the disease is discussed.

Adenoviridae↗