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I D Pawlowski

Publications and source records attributed to I D Pawlowski.

4 recordsLinked to original sources
PubMed1988

The development of nonradioactive total genomic probes for strain and egg differentiation in taeniid cestodes.

Purified total genomic DNA labeled with photobiotin was used as a probe for the identification and differentiation of strains of Echinococcus granulosus and to distinguish between the morphologically identical eggs of 2 taeniid species. Such biotinylated probes are easily prepared and can be utilized effectively for rapid screening and identification of taeniid isolates by dot and Southern blotting. The use of total genomic DNA probes in high stringency hybridizations obviates the need for cloned sequences and has great potential for field use.

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PubMed1987

Taenia hydatigena: isolation of mitochondrial DNA, molecular cloning, and physical mitochondrial genome mapping.

Mitochondrial DNA was isolated from Taenia hydatigena, T. crassiceps, and Echinococcus granulosus using a cetyltrimethylammonium bromide precipitation technique. The technique is simple, rapid, reproducible, and does not require extensive high speed ultracentrifugation. The advantage of using mitochondrial DNA from taeniid cestodes for comparative restriction analysis was demonstrated. Mitochondrial DNA of T. hydatigena was isolated as covalently closed circular molecules. These were linearized by single digestion with BamHI and the molecular weight was estimated from the linear form of 17.6 kb. The mitochondrial DNA of T. hydatigena is therefore similar in size and structure to that of many other animal species. The entire mitochondrial genome was cloned into pBR322 in Escherichia coli and a restriction map of the recombinant molecule was constructed. The potential of using the cloned mitochondrial genome as a probe in speciation studies as well as for providing functional information on the role of the cestode mitochondrion is discussed.

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