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Biomedical subjects

I Deconinck

Publications and source records attributed to I Deconinck.

5 recordsLinked to original sources
PubMed1983

Increase of neurophysin II serum levels in chronic alcoholic patients: relationship with alcohol consumption and alcoholism blood markers during withdrawal therapy.

Neurophysin II (hNpII) but not hNpI serum levels were higher than normal (greater than 2.85 ng/ml) in 9 of 20 patients admitted to the metabolic ward for alcohol withdrawal therapy; a normalization was observed within the first week of alcohol withdrawal. The higher levels of hNpII were felt to reflect alcohol impregnation for the following reasons: 1) on admission, levels of most of the alcoholism blood markers were higher in the group of patients with high hNpII levels than in the group with normal hNpII levels; 2) hNpII levels were correlated with most of the alcoholism blood markers, mainly gamma-glutamyl transpeptidase (r = 0.63, P less than 0.001) in the 20 patients of the study; 3) patients with high hNpII levels admitted to greater alcohol and less anxiolytic drug intake immediately before admission. The physiopathological meaning and hypothetical psychological consequence of this hNpII increase remain to be defined.

Alcohol Drinking↗
PubMed1983

Factors affecting the determination of ionized calcium in blood.

Reproducibility with the same electrode on the same day is expressed by the CV. For standard solutions CV = 0.68%, for blood CV = 1.36%, for serum CV = 0.92%. The measure with 2 different Orion electrodes performed on blood samples on the same day gives no more variation between electrodes than within electrode. Results of Ca++ measured with SS-20 Orion and ICA1 Radiometer electrodes on the samples of normal blood or serum are significantly higher with the ICA1 electrode than with the SS-20 Orion. The use of decreasing concentration of NaCl (170 to 110 mmol/l) in calibration solutions shows an apparent increase of Ca++ concentration in normal blood. Sampling conditions may affect Ca++ results. The pH of peripheric venous blood drawn without torniquet in 57 normal people was 7.34 +/- 0.03 and differs from the normal arterial pH values (740 +/- 0.02). By applying the following equation: Ca++ (pH 7.40) = Ca++ [1-0.53 (7.40 - pH)], Ca++ can be adjusted to pH 7.40. The correction in pathologic condition should, however, take in account the real arterial pH of the subject. We tested if sitting versus recumbent position could modify Ca++ results. The difference was non significant. Heparin, necessary for blood and plasma sampling, may affect the electrode membrane and binds calcium ions. We compared in the same blood sampling the same dilutions of 2 kinds of heparin. Heparin Radiometer: 10 microliters in 1 ml blood (8 UI) and heparin Roche: 10 microliters in 1 ml blood (50 UI) and observed significantly higher Ca++ results with the lower concentration of heparin. Storage of serum 24 hours at -20 degrees C does not alter the Ca++ results. After 7 days at -20 degrees C the Ca++ decreases by 3.6% in average, mainly due to pH increase.

Blood Preservation↗