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I Dorronsoro

Publications and source records attributed to I Dorronsoro.

At least 19 recordsLinked to original sources
PubMed2005

Donepezil-tacrine hybrid related derivatives as new dual binding site inhibitors of AChE.

A new series of donepezil-tacrine hybrid related derivatives have been synthesised as dual acetylcholinesterase inhibitors that could bind simultaneously to the peripheral and catalytic sites of the enzyme. These new hybrids combined a tacrine, 6-chlorotacrine or acridine unit as catalytic binding site and indanone (the heterocycle present in donepezil) or phthalimide moiety as peripheral binding site of the enzyme, connected through a different linker tether length. One of the synthesised compounds emerged as a potent and selective AChE inhibitor, which is able to displace propidium in a competition assay. These results seem to confirm the ability of this inhibitor to bind simultaneously to both sites of the enzyme and make it a promising lead for developing disease-modifying drugs for the future treatment of Alzheimer's disease. To gain insight into the molecular determinants that modulate the inhibitory activity of these compounds, a molecular modelling study was performed to explore their binding to the enzyme.

Acetylcholinesterase↗
PubMed2000

[Confirmation by molecular typing of cross-contamination in a mycobacteria laboratory].

BACKGROUND: Detection of cross-contamination in the laboratory by restriction fragments length polymorphism (RFLP) analysis of Mycobacterium tuberculosis strains. MATERIAL AND METHODS: 224 strains isolated during a five years period were characterized by IS6110 fingerprinting performed (RFLP) by standardized protocols. RESULTS: Four groups of isolates with smear-negative specimens and low number of colony form its units were detected. They were processed in the same batch and day than other smear-positive specimens with identical RFLP patterns. Fifteen patients were involved and the review of four patients' charts showed that they did not have the typical manifestations of tuberculosis. CONCLUSIONS: When M. tuberculosis isolates were obtained from smear-negative specimens, the results of specimens processed in the same batch and the patients' charts should be reviewed. If with these data the possibility of cross-contamination is suspected, the isolates must be analyzed by molecular typing methods.

Clinical Laboratory Techniques↗
PubMed2000

[Effect of the number of samples studied on the diagnosis of tuberculosis].

BACKGROUND: For the microbiological diagnosis of tuberculosis, most standard laboratory guidelines recommend the processing of multiple specimens. This fact added to the nonspecific clinical presentation of many cases, contribute to a low efficiency of the tuberculosis laboratory. The introduction of new procedures have increased the sensibility of the diagnosis and so the cost. The object of our work, was to asses the value of examining 3-6 specimens, in optimizing the diagnosis. METHODS: We retrospectively reviewed 327 specimens from 105 patients with culture-proven tuberculosis, between 1997-1998. All specimens were received in the laboratory within 1 month of the initial diagnosis. RESULTS: Of the 7694 specimens received for acid-fast smear and culture in that period of time, 519 (6.7%) were positive for mycobacteria, many of them without clinical relevance. The average number of specimens processed by patient was 3.1, however 22% of the patients with culture-proven tuberculosis had less than tree specimens submitted at the time of diagnosis and 19% had more than three. In 49.5% of the patients, all the smears were negative and the diagnosis was done by culture. With the first specimen submitted were diagnosed 76% of the patients, and with the second we reach 94.2%, finally, we needed 3 specimens for the diagnosis of 104 from the 105 cases (99%). CONCLUSIONS: With the better sensibility achieved in the detection procedures, the interest in the improvement of the efficiency in the tuberculosis laboratory should consider the contribution of the specimen number on the diagnosis. If studying only one specimen we could fail 25% of the early diagnosis, we have rarely found more than two specimens being of diagnostic value. As the time for detecting a positive culture has decrease with the use of liquid media and probes for identification, we would recommend to submit only two specimens for the routine diagnosis and see the patient again 1 moth later. If in this time, the laboratory has'nt obtain any positive result and the clinician still suspect a tuberculosis, a second set of specimens can be submitted. With this measures, laboratory that received a great number of specimens, could assume the work of small laboratory without increasing there work and budget, and the diagnosis of tuberculosis will be optimized.

Clinical Laboratory Techniques↗
PubMed1997

[Cutaneous infections by Mycobacterium marinum. Description of 3 cases and review of the literature].

BACKGROUND: Human infection by Mycobacterium marinum is infrequent and is currently reported in association with the management of aquariums. The epidemiologic, clinical and microbiologic characteristics of three cases in our hospital are herein reported. METHODS: Three cases of cutaneous infection by M. marinum attended in our hospital from February 1993 to October 1994 are described. RESULTS: The three patients were related with the management of aquariums. In two cases the clinical presentation was of sporotricoid cutaneous dissemination, while the third patient presented a localized cutaneous form. The lesions were localized in the upper right extremity in the three cases. The delay in diagnosis was important in two of the cases. Cure, or improvement, were achieved in three cases with' treatment; one with cotrimoxazol, another with first line tuberculostatic drugs and another did not receive treatment. CONCLUSIONS: Human infection by M. marinum is currently described is association with the management of aquariums. The infection is usually cutaneous, alone or well disseminated, with invasion of deep tissue occasionally observed. The lesions are generally found on the upper extremities. Diagnostic delay is frequent. The most adequate treatment appears to be the combination of rifampicin plus etambutol or cotrimoxazol alone, with cases of spontaneous remission also being observed.

Adult↗
PubMed1996

[Hemorrhagic colitis caused by verotoxigenic Escherichia coli. Presentation of 9 cases].

OBJECTIVE: To describe the clinical and epidemiological characteristics of nine patients with enteritis caused by verocytotoxin-producing E. coli O157. PATIENTS AND METHODS: Clinical data of patients was collected retrospectively, the isolated strains were tested for verotoxin production (VT) using Vero cell culture line, and presence of VT1 and VT2 gene sequences was detected using amplification techniques (PCR), biotype was also determined using twelve biochemical tests, and genomic macrorestriction profile (PFGE). RESULTS: The patients' age ranged from 11 months to 70 years. The mean duration of diarrhea was 4.7 days. All patients but one had abdominal cramps, seven of nine reported hemorrhagic stools and six had fever. Three patients were affected of haematologycal neoplasia and two of them developed hemolytic-uremic syndrome as a complication. All strains produced VT2 and two of them also produced VT1. Epidemiological link between patients has not been established. Three different biotypes had been distinguished between the nine isolated strains. All but two had different macrorestriction profiles. DISCUSSION: The results obtained showed that clinical manifestations are rather inespecific, including fever (6/9 patients) and there is high association of severe complications. The heterogeneity in PFGE results obtained confirms that the cases are not related.

Adult↗
PubMed1996

[Epidemiology of gastroenteritis by Salmonella (1983-1994)].

BACKGROUND: The incidence of Salmonella enteritidis infection has been study in order to determine their epidemic spread from 1983 to 1994, and the impact of the measures introduced to attempt its control. METHOD: Review of the stool cultures positives for enteric pathogens obtained in the Hospital de Navarra from 1983-1994. RESULTS: Looking at the isolation rates of the different enteric pathogens from 1983 to 1994, a sharp increase of S. enteritidis was recorded on 1985 as a consequence of several outbreaks associated with the consumption of mayonnaise elaborated with row eggs. From 409 strains of Salmonella isolated that year, 302 were serotype enteritidis, this means a 7.5 fold rise since the preceding year in which the prevailing serotype was S typhimurium. From 1985 through 1991 the incidence of S. enteritidis stand high, and pick again on 1990. Since then, a decline has been observed, and in 1994 the number of Salmonella cases recorded were about those on 1984, however, S. enteritidis is now the prevailing serotype. CONCLUSIONS: The egg-borne S. enteritidis epidemic emerged as a major public health problem in Navarra in 1995, associated to the consumption of home made mayonnaise. The measures introduced by local Public Health authorities to attempt control of non pasteurized mayonnaise were insufficient. Evidence from other places links human infections to vertical transmission from breeding flocks to layers. And makes of S. enteritidis infection an international challenge. The severity of the lived experience demands a time-temperature control of eggs in the interval from purchaser to consumer and a more generalized use of pasteurized egg products, no only in the industry but also in the household.

Disease Outbreaks↗
PubMed1984

Studies on the polysaccharide B and native haptene of Brucella and Yersinia enterocolitica serotype 9.

Trichloroacetic (TCA) extraction of B. melitensis 115, or hot saline extraction of B. melitensis 16M yields a polysaccharide component that can be purified free of protein and lipopolysaccharide by gel filtration and TCA precipitation. Antibodies to this polysaccharide can be detected in sera of rabbits infected with virulent Brucella but not with B. abortus 19. Similarly, cattle with active brucellosis and humans with acute brucellosis develop antibodies against this polysaccharide. After extraction and purification, this polysaccharide does not elicit antibody response in rabbits or mice. It does not fix the complement, does not bind to red cells or to polystyrene plates.

Animals↗
PubMed1983

Rapid identification of Serratia marcescens by coagglutination.

All 20 O serotypes of Serratia marcescens produce a common, soluble antigen. Crude antigen was obtained by ammonium sulfate precipitation of spent growth medium, antiserum was produced in rabbits, and a coagglutination test for rapid identification of S. marcescens was developed. A total of 701 clinical isolates of gram-negative bacilli were examined, and a 100% correlation between biochemical identification of S. marcescens and identification by coagglutination was found.

Agglutination Tests↗
PubMed1982

[Enteritis caused by Campylobacter fetus in Navarre].

C. fetus ss jejuni was demonstrated in stool samples from 17 patients, among 605 studied, using the selective media of Skirrow. 13 of them were children, 11 two years' old or younger, and 4 were adult patients. The frequency of isolation of C. fetus ss jejuni in stool cultures has been greater than the isolation of Y. enterocolitica serotype 3, S. sonnei and E. coli, and has been only superated by the isolation of microorganisms belonging to the generus Salmonella.

Adolescent↗