Use of 6,000 IU vitamin A during early pregnancy without teratogenic effect.
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Congenital Abnormalities↗
Biomedical subjects
I Dudas
Publications and source records attributed to I Dudas.
The effect of periconceptional multivitamin-mineral supplementation on vertigo, nausea and vomiting in the first trimester of pregnancy.
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Female↗
Colony counting on hydrophobic grid-membrane filters.
A device to facilitate manual scoring of hydrophobic grid-membrane filters (HGMF) is described. Variations in scores were generally less than 2.5% between 41 analysts from six laboratories, who, using the apparatus, scored a set of five specimen HGMF in different ways, and there was good agreement between scores from positive and negative grid-cell counts by each analyst. A scoring procedure for use in routine microbiological analysis, suitable for HGMF at various degrees of saturation, is recommended.
Bacteriological Techniques↗
Membrane filtration of food suspensions.
Factors affecting the membrane filtration of food suspensions were studied for 58 foods and 13 membrane filters. Lot number within a brand, pore size (0.45 or 0.8 micrometer), and time elapsed before filtration had little effect on filterability. Brand of membrane filter, flow direction, pressure differential, age (microbiological quality) of the food, duration of the blending process, temperature, and concentration of food in the suspension had significant and often predictable effects. Preparation of suspensions by Stomacher (relative to rotary blender) addition of surfactant (particularly at elevated temperature) and prior incubation with proteases sometimes had dramatic effects of filterability. In contrast to popular opinion, foods can be membrane filtered in quantities pertinent to the maximums used in conventional plating procedures. Removal of growth inhibitors and food debris is possible by using membrane filters. Lowering of the limits of detection of microorganisms by concentration on membrane filters can be considered feasible for many foods. The data are particularly relevant to the use of hydrophobic grid-membrane filters (which are capable of enumerating up to 9 X 10(4) organisms per filter) in instrumented methods of food microbiological analysis.
Bacteria↗
Automated food microbiology: potential for the hydrophobic grid-membrane filter.
Bacterial counts obtained on hydrophobic grid-membrane filters were comparable to conventional plate counts for Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus in homogenates from a range of foods. The wide numerical operating range of the hydrophobic grid-membrane filters allowed sequential diluting to be reduced or even eliminated, making them attractive as components in automated systems of analysis. Food debris could be rinsed completely from the unincubated hydrophobic grid-membrane filter surface without affecting the subsequent count, thus eliminating the possibility of counting food particles, a common source of error in electronic counting systems.
Bacteria↗
Two stomacher accessories.
Two useful and easily constructed accessories for the Colworth Stomacher blender are described. The first automatically opens and holds Stomacher bags above a balance of weighing in the sample. The second stores bags on the bench between manipulations and opens and holds them as required for pipetting, etc.
Microbiological Techniques↗