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I Ebihara

Publications and source records attributed to I Ebihara.

At least 109 records · Page 6Linked to original sources
PubMed1991

Proto-oncogene expression in peripheral blood mononuclear cells in IgA nephropathy.

We have investigated the expression of proto-oncogenes in mononuclear cells obtained from patients with IgA nephropathy using a RNA hybridization technique. Patients with IgA nephropathy expressed more c-myc, c-raf, c-fos, and c-jun proto-oncogene RNA than did normal controls. However, no significant expression of c-N-ras, c-mos or c-myb genes was found in the mononuclear cells of these patients. When the amount of urinary protein excretion was used as an indicator of disease activity (greater than 1 g/day), a positive correlation was found between c-myc, c-raf, c-fos, and c-jun expression and urinary protein excretion (P less than 0.01). The expression of these genes correlated also with the serum IgA concentration (P less than 0.01), IgA immune complex (P less than 0.01), and histopathological changes in renal tissues obtained from patients with IgA nephropathy (P less than 0.01). The results of this survey suggest that abnormally regulated proto-oncogene expression in mononuclear cells may play an important role in the progression of IgA nephropathy and may be useful as an indicator of disease activity and/or prognosis.

Adolescent↗
PubMed1991

Effects of methylprednisolone on glomerular and medullary mRNA levels for extracellular matrices in puromycin aminonucleoside nephrosis.

We examined the effects of methylprednisolone (MPSL) on type IV collagen, laminin and heparan sulfate proteoglycan (HSPG) mRNA levels in the renal glomeruli and medulla of puromycin aminonucleoside (PAN) nephrosis. mRNA levels encoding for type IV collagen and laminin increased markedly, whereas those for HSPG decreased significantly in glomeruli of PAN nephrosis. Administration of MPSL partially ameliorated the abnormal gene expression for basement membrane components. Furthermore, we showed that medullary mRNA levels for all these basement membrane components decreased with age in PAN nephrosis with or without MPSL treatment, suggesting that neither PAN nor MPSL has any effect on basement membrane component mRNA levels in the renal medulla. In contrast, mRNA levels for the interstitial collagens including alpha 1 (I) and alpha 1 (III) chains in glomeruli showed little change with or without MPSL treatment, whereas those in medulla increased significantly in PAN nephrosis when compared with the control. MPSL ameliorated the abnormal gene expression of alpha 1 (I) and alpha 1 (III) collagen in renal medulla. These results indicate that PAN affects both glomerular mRNA encoding for basement membrane components and medullary mRNA encoding for interstitial collagens, and that MPSL has marked effects on the amelioration of abnormal gene expression in both glomeruli and medulla of PAN nephrosis.

Animals↗
PubMed1991

Increased expression of proliferating cell nuclear antigen mRNA in peripheral blood mononuclear cells from patients with IgA nephropathy.

Proliferating cell nuclear antigen (PCNA)/cyclin is an intranuclear polypeptide antigen whose appearance correlates with the proliferative state of cells. The authors investigated the expression of PCNA from peripheral blood mononuclear cells (PBMC) in 23 patients with IgA nephropathy and 10 healthy age-matched controls, using ribonucleic acid hybridization techniques. The majority of patients with IgA nephropathy (22 of 23 patients) showed elevated PCNA expression in PBMC, while no PCNA expression was detected in PBMC of normal controls. A positive correlation was noted between PCNA expression of PBMC and glomerular injuries and PCNA expression and urinary protein excretion. Sixty-three percent of patients with grade III and IV histological findings showed strong PCNA (more than ) expression in their PBMC. The urinary protein excretion in patients who showed more than ( ) PCNA expression was more than 2.5 g/d, while that in patients with less than (+) PCNA expression was less than 1.0 g/day. These findings indicate that abnormally regulated PCNA expression in PBMC may play an important role in the progression of IgA nephropathy, and that PCNA expression in PBMC may be a useful indicator of disease activity.

Adolescent↗
PubMed1991

Increased steady-state levels of mRNA coding for extracellular matrix components in kidneys of NZB/W F1 mice.

The present study was carried out to determine how mRNA levels of extracellular matrix (ECM) components including alpha 1(IV) chain, laminin A, B1 and B2 chains, heparan sulfate proteoglycan (HSPG), alpha 1(I) chain, and alpha 1(III) chain are regulated in the kidney of NZB/W F1 mice. Messenger RNA levels for ECM genes except for laminin A chain increased significantly with the progression of nephritis in NZB/W F1 mice. In the NZW kidneys, however, the mRNA levels for alpha 1(IV) chain, laminin B1 and B2 chains, and HSPG declined markedly with age, whereas those for alpha 1(I) and alpha 1(III) chains showed little difference throughout the experimental period. Messenger RNA levels of beta-actin remained constant, and those of laminin A chain could not be detected in either control or diseased kidneys. Immunofluorescent microscopy showed that the intensity and distribution of staining of collagen IV, laminin, and HSPG in the glomeruli of NZB/W F1 mice increased markedly with the progression of disease. Types I and III collagen were not detected in the glomeruli of NZB/W F1 mice by immunofluorescence until 24 weeks of age, after which increased amounts of these collagens were found in the glomeruli and interstitium with progression of disease. These results suggest that increased levels of mRNA coding for ECM components and increased accumulation of these proteins may contribute to a cascade of events, leading to chronic renal injury in lupus nephritis.

Animals↗
PubMed1991

Perforin mRNA expression in the inflamed tissues of NZB/W F1 lupus mice decreases with methylprednisolone treatment.

Perforin is one of the important cytolytic factors in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. In this study, the authors examined perforin mRNA levels in the kidney, spleen, liver, lung, heart, and brain of NZB/W F1 lupus mice and NZW mice. Perforin mRNA levels in the kidney, spleen, liver, and lung of NZB/W F1 mice increased significantly with age, whereas those in the heart and brain of NZB/W F1 mice showed little change between 2 and 10 months of age. In all tissues examined in NZW, control mice perforin mRNA levels showed little change during the experimental period. In addition, the authors examined the effect of methylprednisolone (MPSL) on perforin gene expression in the tissues of NZB/W F1 mice. MPSL ameliorated the increase in perforin mRNA levels in the kidney, spleen, liver, and lung of NZB/W F1 mice. These findings suggest that perforin may contribute to tissue injuries in autoimmune lupus mice and that MPSL may be effective in lupus partly by decreasing perforin expression.

Animals↗
PubMed1991

Modulation of basement membrane component gene expression in glomeruli of aminonucleoside nephrosis.

The present studies were designed to analyze mRNA levels of basement membrane components including collagen IV, laminin, and heparan sulfate proteoglycan (HSPG) in the course of puromycin aminonucleoside (PAN) nephrosis. mRNA levels for alpha 1 (IV) chain; laminin A, B1, and B2 chains; and HSPG were measured in glomeruli of PAN nephrotic rats 0, 2, 8, 14, and 20 days after PAN injection. In the nephrotic stage of PAN nephrosis (on the 8th day), mRNA levels for alpha 1 (IV) chain and laminin A, B1, and B2 chains increased, whereas those for HSPG decreased. The anionic sites in glomerular basement membrane stained by polyethyleneimine were smaller in size and fewer in number in PAN nephrotic rats than they were in control rats. In the remission stage of PAN nephrosis (on the 20th day), however, mRNA levels for alpha 1 (IV) chain and laminin A, B1, and B2 chains decreased, whereas mRNA levels for HSPG increased. Polyethyleneimine aggregates in this stage appeared to be larger and more intense than those in the nephrotic stage. These results indicate that the expression of basement membrane genes for alpha 1 (IV), laminin, and HSPG was abnormally regulated in PAN nephrosis and that this abnormal gene regulation might contribute to the development of proteinuria.

Animals↗
PubMed1989

[A pathological study of carcinoma of the lung and pneumoconiosis].

Pathological studies on lung carcinoma associated with pneumoconiosis were made in order to clarify the etiological significance of pneumoconiosis in carcinoma of the lung. Between 1960 and 1986, the author evaluated approximately 450 autopsies of pneumoconiosis. Of these, 150 were consecutive autopsies in our over laboratory and the remaining 300 were kindly provided by other hospitals. Carcinoma of the lung was seen in 48 of the autopsies. Pathological studies determine histologic type, cancer site, and severity of pneumoconiosis were performed in the 48 cases. The severity of pneumoconiosis was determined by the extent of progressive massive fibrosis (PMF). Simple pneumoconiosis was classified as mild, pneumoconiosis with PMF smaller than segmental region as moderate and pneumoconiosis with PMF larger than lung segment as severe. Carcinoma of the lung was seen in 25 of our own consecutive autopsies, an incidence of 17.9%. The incidence of lung carcinoma was high among cases of mild pneumoconiosis and low among cases of severe pneumoconiosis. Overall, the predominant cancer was squamous cell carcinoma (54.2%) followed by small-cell carcinoma (22.9%) and adenocarcinoma (14.6%). There was a clear trend that most of the squamous cell carcinomas were found in the larger airways, whereas the adenocarcinoma was found only in the peripheral lung tissues. In cases of mild pneumoconiosis, the majority of tumors arose in the right, upper and larger airways. On the other hand, in cases of moderate and severe pneumoconiosis, more tumors arose in the left, lower and peripheral lung areas.(ABSTRACT TRUNCATED AT 250 WORDS)

Adenocarcinoma↗
PubMed1988

Altered mRNA expression of basement membrane components in a murine model of polycystic kidney disease.

Basement membranes surround the renal tubules and have been shown to limit their distension in vitro. Therefore, it has been postulated that a defect in a basement membrane component(s) underlies the pathogenesis of polycystic kidney disease. Here we have studied a murine model of congenital polycystic kidney disease and found by immunohistology, that the components of the peri-cyst basement membrane appeared to diminish with time. We also measured mRNA levels for collagen IV and laminin, and found a different pattern than in the normal mouse kidney. In normal kidneys, mRNA levels for the B1 and B2 chains of laminin were maximal at birth, and at 1 week for the alpha 1(IV) chain of collagen IV. With all three chains, the levels then rapidly declined. In contrast, mRNA for the alpha 1(IV) chain in congenital polycystic kidneys was half normal 1 week after birth and then increased. Laminin B1 and B2 chain mRNA's were 80% of normal at 1 week but were maintained at that level. As a control, beta-actin mRNA was examined and found to remain constant in both normal and diseased kidneys. In situ hybridization of cRNA probes for the alpha 1(IV) chain confirmed that cells associated with cysts were the principal source of expression of these basement membrane mRNAs. Thus, there exists an abnormal regulation of basement membrane gene expression in congenital polycystic kidney disease. The first stage is characterized by reduced levels of expression. In the second stage, the levels are abnormally high, perhaps representing a compensatory synthesis of basement membrane as cysts enlarge.

Animals↗
PubMed1987

Genes for basement membrane proteins are coordinately expressed in differentiating F9 cells but not in normal adult murine tissues.

We have obtained cDNA clones coding for the A, B1, and B2 chains of laminin by screening a cDNA library prepared from mouse EHS tumor poly(A)RNA in the lambda gt11 expression vector with polyclonal antibody against denatured laminin. These cDNA clones were used in combination with a cDNA clone coding for the alpha 1 type IV collagen chain to study the regulation of genes for these basement membrane proteins in retinoic acid-induced differentiating mouse F9 teratocarcinoma cells and in various adult murine tissues. The levels of mRNA for the laminin A, B1, and B2 chains and for the alpha 1 type IV collagen chain were increased simultaneously and reached a maximum at almost the same time during the differentiation of F9 cells, suggesting coordinate expression in these cells. The tissue levels of mRNA encoding for the basement membrane components, however, varied considerably. The highest level of the B1 chain mRNA was observed in kidney, whereas, the levels of mRNA for A and B2 chains were highest in heart. Almost the same levels of expression of the alpha 1(IV) collagen mRNA were found in kidney, lung, and heart. The results indicate that the expression of genes for the basement membrane proteins is not coordinately regulated in these tissues. It is thus possible that different subunit structures of the laminin molecule may exist in tissues.

Animals↗