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I Erzen

Publications and source records attributed to I Erzen.

29 records · Page 2Linked to original sources
PubMed1990

Muscle fibre types in the human vastus lateralis muscles: do symmetrical sites differ in their composition?

Muscle samples obtained from distinct symmetrical sites of left and right vastus lateralis muscles from 10 young healthy males were analysed. A computer-aided system was applied for the determination of muscle fibre types I, IIA, IIB and IIC and for their quantitative analysis. No statistically significant differences were established regarding percentage and surface area of all the four fibre types studied. In 2 cases of IIA and in one case of IIB fibre type significantly different diameters were measured between the left and right muscle. Regarding percentage and surface area no differences were proved between superficial and deep layers of the muscles observed. Fibre diameter but differed significantly in many muscles, there was no rule about whether it was larger in superficial or in deep muscle layers. One biopsy sample can not represent the whole muscle, especially as far as the relative number and surface area of the type IIA and IIB fibres are concerned. A slight asymmetry in fibre diameters of biopsy samples from both legs does not indicate any pathological change and can still be taken for normal.

Adult↗
PubMed1989

Histophotometry--the method of choice in quantifying dehydrogenase histochemistry.

Succinate, malate, and lactate dehydrogenase were demonstrated histochemically and measured histophotometrically in the heart and skeletal muscle (m. extensor digitorum longus and m. soleus) of rats at different ages. To prove the value of histophotometry, the enzymes of the tissues were estimated biochemically. The gel film technique cannot sufficiently prevent the diffusion of the soluble enzymes (malate-, lactate dehydrogenase) out of the tissue sections. Because of the different mobility, various isoenzymes, histophotometry cannot give reliable results. But, as far as membrane-bound dehydrogenases (succinate dehydrogenase) are concerned, histophotometry is the method of choice for basic measurements as in routine practical work, especially with tissues where the enzyme activities are heterogeneously distributed, e.g. in different types of muscle fibres in skeletal muscles.

Aging↗
PubMed1987

Acute renal failure in leptospirosis--a 12-year survey.

From the beginning of 1974 to the end of 1985 141 persons in Slovenia (population 1.9 million) contracted leptospirosis 49 patients were 50 years old or more. All the patients have survived. 8 male patients aged from 18 to 44 had more severe acute renal failure (ARF), 5 of whom were treated with hemodialysis. The authors found that this 100% survival rate did not agree with the findings of many other researches which quote a relatively high mortality rate among older patients. In all 8 patients kidney function was good 1 year or more after ARF. The authors consider that the survival of patients with ARF depends on early and appropriate supportive treatment, which also includes intensive hemodialysis.

Acute Kidney Injury↗
PubMed1986

The correlation between histophotometrical and biochemical myosin-ATPase measurements in the myocardium and striated muscle of the rat.

To prove the correlation between histophotometrical and biochemical data of myosin-ATPase-activity, the change of enzyme activity during development was measured in the myocardium, masseter muscle, and tensor fasciae latae of rats. Both methods were applied to the same material. To avoid errors due to varying section thickness in each cutting and staining procedure, a reference material (e.g. heart muscle) was used, taken from one and the same animal. The correlation between histophotometrical and biochemical estimations was very good, histophotometrical arbitrary units being used. ATPase-activity in heart muscle during development, slightly decreases in masseter muscle and more in tensor fasciae latae. The ATPase-activity in the latter was higher than in masseter muscle. This is in good agreement with the physiological, biochemical, and histochemical classification.

Adenosine Triphosphatases↗
PubMed1985

The nonspecificity of the lead method for the histochemical demonstration of adenosine triphosphatases in human skeletal muscle fibres.

The lead method for the histochemical demonstration of presumptive mitochondrial adenosinetriphosphatase was applied to biopsy and autopsy samples of the human vastus lateralis muscle. The effect of p-chloromercuribenzoate and of Triton X-100 was tested microdensitometrically and the activity of 'mitochondrial' ATPase was compared to the activity of enzymes of the oxidative metabolism succinic dehydrogenase and NAD-tetrazolium reductase. It is concluded that the ATPase activity displayed is not mainly mitochondrial. In autopsy material, it seems to be predominantly myofibrillar.

Adenosine Triphosphatases↗
PubMed1984

Lysosomal and energy enzyme activities in hypertrophied rat soleus muscle after denervation.

Muscle hypertrophy was induced in the soleus muscle of young rats by tenotomy of the gastrocnemius and plantaris muscles. Three and 7 days afterwards the sciatic nerve was sectioned. The loss of weight of muscles subjected to this combined procedure three days after denervation was 30-40%. Lysosomal enzyme activities (acid phosphatase, alpha-glucosidase, beta-galactosidase and N-acetyl-beta-D-glucosaminidase) and energy enzyme activities (lactate dehydrogenase, LDH, triose-3-phosphate dehydrogenase, TPDH , D-hexokinase, HK and citrate synthase, CS) were determined 3 days after denervation, 3, 7 and 10 days after hypertrophy had been induced and 3 days after denervation of hypertrophying muscles on day 3 and 7. Normal non-operated rats of corresponding body weight served as controls and their enzyme activities were estimated on the same day. In the course of muscle hypertrophy, the 4 lysosomal enzyme activities increased progressively. Although 3 days' denervation of control muscles did not alter lysosomal enzyme activities, denervation of hypertrophying muscles greatly enhanced the activity of these enzymes. Enzymes of energy metabolism were affected to a lesser degree. The results suggest that denervation of hypertrophying muscles causes more extreme changes in muscle weight and lysosomal enzyme activities than denervation alone. The possible implications of this finding are discussed in relation to the rapid atrophy.

Animals↗
PubMed1983

Enzyme activity pattern in developing mouse brain in situ in embryonic brain aggregated cells at 37 degrees C and 0 degree C.

In aggregates of nervous tissue, cultivated for 1--7 days at 0 degree C and 37 degrees C, respectively, the activities of seven enzymes of energy liberating metabolism were estimated, in order to evaluate their metabolic "profiles" and changes during cultivation. The enzymes used as markers of different pathways of energy liberation from substrates were: lactate dehydrogenase - LDH - (EC 1.1.1.27), triose-3-phosphate dehydrogenase - TPDH - (EC 1.2.1.12), glycerol-3-phosphate dehydrogenase - GPDH - (EC 1.1.1.8), hexokinase - HK - (EC 2.7.1.1.), malate:NAD dehydrogenase - MDH - (EC 1.1.1.37), citrate synthase - CS - (EC 4.1.3.7), and 3-hydroxyacetyl CoA dehydrogenase - HOADH - (EC 1.1.1.35). During the cultivation, some changes in the metabolic "profiles" were observed. Although some of these changes as well as the differences between the cultivation at 0 degree C and 37 degrees C, were statistically significant, they were not greater than the variations between different samples of any tissue taken at different times. They were not, therefore considered to be of major significance. However, all the aggregates exhibited "profiles" characteristic for the nervous tissue, with relatively very high activity of HK, high activity of MDH and CS (carbohydrate breakdown) and low activity of GPDH and HOADH (lipid catabolism).

Animals↗
PubMed1981

Activity of some enzymes of energy metabolism during denervation and reflex atrophy in rat slow and fast muscles.

The loss of muscle weight in the soleus (SOL) and extensor digitorum longus (EDL) muscles was compared after denervation and in the course of reflex muscle atrophy induced by unilateral fracture of metatarsal bones of the paw and local injection of 0.02 ml turpentine oil subcutaneously. This so-called reflex atrophy is significantly greater after 3 days than that after denervation. Seven days after the nociceptive stimulus, reflex and denervation atrophy are grossly similar in both muscles. This also applies in case that the nociceptive stimulus had been repeated on the third day. The EDL:SOL enzyme activities of energy supply metabolism reflect the differences between a glycolytic-aerobic (EDL) and predominantly aerobic type (SOL) of muscle. No consistent changes were found in either type of atrophy after 3 days. In 7 days' denervation, the activity of hydroxyacetyl-CoA-dehydrogenase (HOADH) and citrate synthase (CS) was decreased in the SOL, while glycerolphosphate:NAD dehydrogenase (GPDH) was enhanced. In the EDL, the activity of triosephosphate dehydrogenase (TPDH), GPDH, malate dehydrogenase (MDH), CS and HOADH was decreased. Acid phosphatase (AcP) was greatly increased in both muscles. Seven days after application of the nociceptive stimulus, all enzyme activities were altered in a grossly analogous manner as after denervation.

3-Hydroxyacyl CoA Dehydrogenases↗
PubMedDate not supplied

A novel staining method for quantification and 3D visualisation of capillaries and muscle fibres.

The aim of this study was to introduce a combined fluorescent staining that clearly demonstrates capillaries and distinguishes them from the basal lamina of muscle fibres in skeletal muscle tissue. The triple staining with CD31, Griffonia (Bandeira) simplicifolia lectin (GSL I) and laminin efficiently distinguishes vascular endothelium from the basal lamina of skeletal muscle fibres in physiological and pathological conditions. The presented triple staining method has several advantages, which facilitate quantitative analysis of the capillary network, and its relation to individual muscle fibres.

Animals↗
PubMedDate not supplied

Histochemistry of abductor hallucis muscle in children with idiopathic clubfoot and in controls.

The histochemical composition of the abductor hallucis (AH) muscle was investigated in 39 children with idiopathic clubfoot (CF), aged 0-11 years, and in 42 controls. In the youngest group of patients (0-2 years) the percentage of type 1 (slow twitch, tonic) fibers was significantly higher than in controls. In older groups, there was no difference between patients and controls. The relative predominance of type 1 fibers could be due to immobilization, passive shortening or stretching, or primary overactivity of this muscle in CF promoted by an unknown neural factor. None of these interpretations could be proven.

Biopsy↗