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I F Zhimulev

Publications and source records attributed to I F Zhimulev.

114 records · Page 7Linked to original sources
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[Creation of a new construct for cloning DNA and modeling the structure of Drosophila polytene chromosomes].

Modification of P element-based transformation vector pCaSpeR3New yielded a new construct, pICon, which contains the structural region of the Escherichia coli lacZ, the adjacent 5' and 3' regulatory regions of hsp70, pUC19, and two tandem FRTs. Owing to the hsp70 promoter, the pICon insertion site may be localized on polytene chromosomes after heat shock by light or electron microscopy. The pUC19 sequence with a polylinker allows cloning of the genomic sequence adjacent to the 3' end of pICon by the rescue of the P-element target. Functional FRTs allow insertion or deletion of various DNA fragments. The construct is large (22046 bp), forms easily detectable structures in polytene chromosomes, and may be used to study the structural and functional organization of the Drosophila melanogaster genome, in particular, to elucidate the causes of banding pattern formation. To map the molecular boundaries of interband 3C6/C7, the DNA sequence of this region was cloned between the two FRTs.

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[An analysis of the DNA sequence in the region of the breakpoint of the T(1;2)dor(var7) translocation inducing the mosaic-type position effect in Drosophila melanogaster].

The sites of location of (AC/GT) minisatellite sequence were detected in 2B region of X-chromosome of Drosophila melanogaster. The distribution of (AC/GT) repeats coincided with the location of one-half of the mapped breakpoints of known rearrangements within this region. Analysis of sequences neighbouring the breakpoints of the rearrangement T(1;2)dor(var7) demonstrated that the breaks between two sequences of (AC/GT) repeats occurred at a distance 80 and 156 bp, respectively, but there were no (AC/GT) repeats in r combination directly. T-rich sequences 25 bp long as well as short tandem repeats were found near the breakpoints. The pentanucleotide repeat (CTGTT)10 is located at a distance of 660 bp from the breakpoint. It differs by one nucleotide from the sequence of the GTGTT satellite attached to the 1A-2B fragment of X-chromosome as a result of rearrangement.

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