Biomedical subjects
I Flemming
Publications and source records attributed to I Flemming.
Characterization of yeast strains of the genera candida, Hansenula, Kluyveromyces, Pichia, Rhodotorula, and Saccharomyces by mixed-dye fluorometry.
An analytical method in which we used the selective adsorption of several fluorophores by yeast cells is described. The suitability of using binary mixtures of 1-pyrene butyric acid, 3,6-dimethylamino acridine, 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid, and rhodamine B isothiocyanate for the characterization and identification of microorganisms was tested with 98 yeast strains belonging to the genera Candida, Hansenula, Kluyveromyces, Pichia, Rhodotorula, and Saccharomyces. The application of multivariate statistical methods and pattern recognition methods to the allocation of the yeast strains into genus-species-strain structures and to a comparison of fluorescence data sets for differentiation and identification purposes showed the usefulness of the method.
Fibrin sealant in punch hair grafting.
In this study it was shown that fibrin sealant used in eight consecutive patients undergoing their first session of punch hair grafting after sagittal midline excision(s) reduces the hair survival rate by about 2 hairs per graft from 21.74 on the non-glued side to 19.79 on the glued side (p less than 0.01). The authors did not find this sealant beneficial in routine punch hair transplantation but found it to be of some potential value if the grafts are to be left without any dressing immediately postoperatively.
[Brain protection in the surgical reconstruction of the supra-aortic arteries].
The importance of brain protection in surgical handling of supra-aortic arteries may be derived from the specific nature of cerebral blood flow and from the pathophysiology of damaged cerebral vessels. There is a wide range of special protective methods which have proved to be effective, including strict observance of established rules on surgical indication and tactics, maintenance of circulatory homoeostasis, heparinisation, and use of an intra-luminal shunt. Results obtained from operations on 486 patients for occlusion of supra-aortic arteries are discussed, with particular emphasis being laid on the need for intra-operative EEG monitoring. Reference is also made to measures of brain protection in the postoperative phase.
[Interactions between lectins and microorganisms. 1. Determination of the rate of agglutination by measurement of extinction: agglutination of yeast cells (Saccharomyces cerevisiae H155) by concanavalin A].
A simple photometric method for the determination of the agglutination rate of cells by lectins in a continuously stirred suspension is presented. Besides the agglutination rate the method allows the estimation of an average degree of agglutination, i.e. the number of cells per aggregate. The influence of the Con A-concentration, cell number, temperature, and pH on the agglutination rate of Saccharomyces cerevisiae H155 have been studied. The results are of good reproducibility and, therefore, the methods is suitable to describe interactions between cell-bound receptors and receptor-specific proteins, e.g. lectins and antibodies.
The maximization of wound healing with fibrin glue.
Our experience in the application of so-called fibrin glue in 304 patients submitted to a variety of operations in plastic surgery from January 1981 to May 1982 is presented. The results seem to be much better than without this substance and afford greater comfort for the patient. Addition of factor XIII seems to be unnecessary.
[Functional and esthetic considerations in old age. Rhinoplasty as a preventive measure].
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[Oral automatism in acute coma].
Of 98 patients in ai acute state of coma different etiology, 18 (or 18.4 percent) showed series of rhythmic oral motions ("chewing automatisms"). Only one of these patients was observed to develop an apallic syndrome. The characteristic features of chewing automatisms and their concomitant symptoms are described, and the need for delimiting them from more differentiated oral-motor patterns is emphasized. Both forms are of different importance to an assessment of the respective course of disease. The observation of chewing automatisms in acute states of coma does not allow the incipient development of an apallic syndrome to be inferred.
[Irradiation prophylaxis of keloids and cicatricial hypertrophies (author's transl)].
The results obtained for 100 patients by combined surgical and radiation therapy are presented and by means of exemplary cases illustrated. As sson as 24 to 48 hours after excision of the keloid by mostly atraumatic surgical technique, and closing the wound as tension-free as possible, radiation therapy is begun. This takes place under the conditions of surface therapy in single doses of 1.5 Gray two or three times per week up to a total dose of 9 to 12 Gray. Good cosmetic and functional results can be achieved with this technique. The rate of recurrences is extremely low. The radiation load to the patient is so small that it seems justifiable to recommend the combined treatment as the routine method for therapy of keloids. In cases of a known disposition for developing keloids or cicatricial hypertrophies, postoperative radiation therapy is indicated as a prophylactic measure, especially after surgery in the facial region.
[Postoperative psychical disturbances (author's transl)].
Abnormal psychical disturbances after surgical procedures may either be attributed to the experience of illness or to cerebral dysfunction, directly or indirectly including the brain in the process of the disease. The latter results in somatic psychotic states and "Durchgangssyndrom". The postoperative psychosis is not a nosological entity.
[Electroencephalographic longitudinal studies in nontraumatic cerebrovascular processes in childhood].
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[Clinical and electroencephalographic follow-up of consciousness disorders].
A report is given of the experience gained in close-meshed neurological and electroencephalographic observations of unconscious patients suffering from acute and severe brain damage of different aetiology. Only in some cases can the neurological findings be assigned to the midbrain or bulbar brain syndromes - in our material this is possible only after skull-brain traumas and subarachnoid haemorrhage but least of all after hypoxic accidents and brain operations. The vegetative functions often show inconsistency and do not always correspond with the rest of the neurological findings. Selected examples are used to make an assessment of electroencephalography in the interpretation of severe and acute brain damage.
[Differentiation of post-traumatic disturbances of consciousness].
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[Anesthesiological aspects in the pronouncement of death].
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[Classification of laryngotracheal stenosis].
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[Experimental studies on tracheal reconstruction].
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[Ambulatory anesthesia and preoperative care].
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