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I Fogy

Publications and source records attributed to I Fogy.

7 recordsLinked to original sources
PubMed1987

Evolutionary relationships within the human rhinovirus genus: comparison of serotypes 89, 2, and 14.

The complete nucleotide sequence of the genome of human rhinovirus type 89 was determined from the cDNA that had been cloned into Escherichia coli. The genome is 7152 nucleotides long and contains a single large open reading frame of 2164 codons. Translation commences at position 619 and ends 42 nucleotides before the poly(A) tract. The positions of three proteolytic cleavage sites in the polyprotein were determined by N-terminal amino acid sequencing of the capsid proteins; the remainder were predicted from comparisons with other picornaviruses. Extensive similarity between the derived amino acid sequences of human rhinovirus types 89 and 2 was found, whereas the similarity between human rhinovirus types 89 and 14 was considerably less. It is apparent that human rhinoviruses may be more closely related than has been previously thought.

Amino Acid Sequence↗
PubMed1985

Human rhinovirus 2: complete nucleotide sequence and proteolytic processing signals in the capsid protein region.

cDNA clones representing the entire genome of human rhinovirus 2 have been obtained and used to determine the complete nucleotide sequence. The genome consists of 7102 nucleotides and possesses a long open reading frame of 6450 nucleotides; this reading frame is initiated 611 nucleotides from the 5' end and stops 42 nucleotides from the polyA tract. The N-terminal sequences of three of the viral capsid proteins have been elucidated, thus defining the positions of three cleavage sites on the polyprotein. The extensive amino acid sequence homology with poliovirus and human rhinovirus 14 enabled the other cleavage sites to be predicted. Cleavages in the 3' half of the molecule appear to take place predominantly at Gln-Gly pairs, whereas those in the 5' half (including the capsid proteins) are more heterogeneous.

Amino Acid Sequence↗
PubMed1979

[Determination of carbamate pesticides in fruits and vegetables by means of high pressure liquid chromatography (author's transl)].

It is shown that carbamate pesticides can be determined at the ppm level in different kinds of fruits and vegetables using high pressure liquid chromatography. The lower detection limit corresponding to a signal to noise ratio of three is between 0,025 and 0,25 ppm depending on the type of carbamate and plant material. In all cases it is below the maximum permissible value for pesticide residues. Due to the high separating power of high pressure liquid chromatography a simple sample pretreatment procedure can be used. The carbamates are extracted from the biological sample by dichloromethane and injected directly into the liquid chromatograph after replacing the extraction solvent by the mobile phase. In many cases a definite identification and quantitation of the carbamate is possible with a single chromatographic column. Some plants with a more complex matrix require a two-column operation in which the effluent fraction of the first column containing the pesticide is transferred by column switching to a second column in order to achieve a complete separation. The improved resolution in the two-column operation is caused by the relative enrichment effect of the fractionation and the increased column length.

Carbamates↗
PubMed1978

[A method for differentiating between vinegar produced by fermentation and vinegar made from synthetic acetic acid based on the determination of the specific 3H-radioactivity (author's transl)].

The applicability of the specific 3H-radioactivity for the distinction of synthetic and biogenic vinegar was tested. The acetic acid was isolated from the vinegar as calcium acetate, and the calcium acetate was combusted at 830 degrees C to CO2 and H2O. The water was measured either after two destillation steps by liquid scintillation counting or after reduction to hydrogen and reaction to ethane in a gas proportional counter. The difference in the 3H-content between the two types of vinegar in Austria is about 80--100 T.U. Since the level of activity is subject to appreciable annual fluctuations a series of synthetic and biogenic comparison samples always has to be included.

Acetates↗
PubMed1978

[A method for differentiating between vinegar produced by fermentation and vinegar made from synthetic acetic acid based on determination of the 13C/12C-isotope ratio by mass spectrometry (author's transl)].

The 13C/12C-isotope ratio is characteristic for vinegar of fermentation and synthetic origin respectively and used for their differentiation. The acetic acid was isolated from the vinegar as calcium acetate, the calcium acetate was pyrolysed to CaCO3 and the CO2 was released from the CaCO3 with H3PO4. The CO2 was measured in a mass spectrometer with double collector. The difference in the 13C-content between the two varieties of vinegar is 5 0/00; the accuracy of the measurement is between is 0.5 0/00 and 1 0/00 Therefore, addition of synthetic acetic acid in excess of 15--20% to fermentation vinegar can be detected by this method.

Acetates↗
PubMed1977

[A contribution to the distinction of biogenic vinegar and vinegar made from synthetic acetic acid by determining the specific 14C-radioactivity (author's transl)].

The method of Simon et al. [2] for the separation of the acetic acid from vinegar prior to the determination of the specific 14C-radioactivity has been modified. The precipitation as calcium acetate and the preparation of free acetic acid by addition of diphosphoric acid has been replaced by an extraction procedure with diisopropylether which is faster and cheaper. On the Austrian market glacial acetic acid (Merck, p.A.) having the natural spezific 14C-radioactitivity was found. The natural specific 14C-radioactivity is therfore necessary but not sufficient to prove the biogenic origin of vinegar.

Acetates↗