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Biomedical subjects

I G Kim

Publications and source records attributed to I G Kim.

42 records · Page 3Linked to original sources
PubMed1992

The cornified cell envelope: loricrin and transglutaminases.

The cornified cell envelope (CE) of terminally differentiated human epidermis is a complex structure consisting of several defined protein constituents. The CE is the most insoluble component of the epidermis due to crosslinking by disulfide bonds as well as isodipeptide bonds that are formed by the action of transglutaminases (TGases). We have recently determined that loricrin is the major component of CE. We now have isolated and characterized its gene and showed that it has a simple structure with a single intron. We also show that the loricrin gene maps to position 1q21, which, coincidentally, is similar to the location of the profilaggrin and involucrin genes. Human loricrin in 26 kDa and consists of three long glycine-serine-cysteine rich sequence domains that contain quasi-repeating peptides and which form the novel glycine loop motif. These are interspersed by lysine+glutamine rich domains involved in isodipeptide crosslinks. The glycine loops are thought to be involved in organization of epidermal proteins and maintenance of the flexibility of the epidermis. By use of PCR analyses, we have found that human loricrin consists of two allelic size variants, due to sequence variations in the second glycine loop domain only, and these variants segregate in the human population by normal Mendelian mechanisms. Furthermore, there are multiple sequence variants within these two size class alleles due to various deletions of 12 bp (4 amino acids) in the major loop of this glycine loop domain. In order to study the expression and role of TGases in the formation of CE, we have isolated and sequenced cDNA and genomic clones encoding the TGase1 enzyme.(ABSTRACT TRUNCATED AT 250 WORDS)

Amino Acid Sequence↗
PubMed1991

The complete amino acid sequence of the human transglutaminase K enzyme deduced from the nucleic acid sequences of cDNA clones.

In order to study the expression and role of transglutaminases in the formation of the cross-linked cell envelope of human epidermis, we have used a synthetic oligonucleotide encoding the consensual active site sequence of known transglutaminase sequences. By Northern blot analysis, newborn foreskin epidermis expresses three different mRNA species of about 3.7, 3.3, and 2.9 kilobases while normal cultured epidermal keratinocytes express only the 3.7- and 2.9-kilobase species. The largest species corresponds to a known ubiquitous tissue type II or transglutaminase C activity, the smallest corresponds to a known type I or transglutaminase K activity, and the mid-sized component apparently encodes a transglutaminase E activity that has recently been shown to be expressed in terminally differentiating epidermis (Kim, H. C., Lewis, M. S., Gorman, J. L., Park, S. C., Girard, J. E., Folk, J. E. & Chung, S. I. (1990) J. Biol. Chem., in press). Using the active site oligonucleotide as a probe, we have isolated and sequenced cDNA clones encoding the transglutaminase K enzyme. The deduced complete protein sequence has 813-amino acid residues of 89.3 kDa, has a pl of 5.7, and is likely to be an essentially globular protein, which are properties expected from the partially purified enzyme. It shares 49-53% sequence homology with the other transglutaminases of known sequence, especially in regions carboxyl-terminal to the active site, and possesses sequences likely to confer its Ca2+ dependence. Interestingly, its larger size is due to extended sequences on its amino and carboxyl termini, absent on the other transglutaminases, that may define its unique properties.

Amino Acid Sequence↗
PubMed1988

A simple method for the treatment of the inverted nipple.

We introduce a simple method for correcting the inverted nipple in which there is preservation of the lactiferous duct, less surgical invasion, and negligible scar formation. Our method consists of two parts: releasing fibrous tissue around the lactiferous ducts, and keeping the nipple in the everted position with a bolster suture and milk suction pump.

Adolescent↗
PubMed1985

Guided dilators for the common bile duct.

The use of the flexible guide dilators for the common bile duct has remarkably reduced the amount of manipulation, trauma and handling of the duct during exploration. As a result, there has been the additional advantage of an over-all reduction in the time required for operating. This simple technique has been successfully performed in approximately 80 instances.

Common Bile Duct↗
PubMed1985

Endoscopic guided intubation of long nasointestinal decompression tube.

Surgeons have long been faced with the problem of intubating long nasointestinal tubes through the pylorus of the stomach when intestinal obstructions are present. The procedure described herein provides a direct view of intubation, endoscopically, rather than a blind approach fluoroscopically. This is achieved in every instance through the use of a guided wire inserted through an endoscope with a sleeve type, long nasogastrointestinal decompression tube. The procedure has proved to be advantageous because of the time saved by providing for rapid passage and prompt decompression which also eliminates trips and calls to the radiology department. Also, the improved condition of the patient prior to operation and the decreased amount of radiation that the patient is exposed to are advantages.

Gastroscopy↗