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Biomedical subjects

I G Morris

Publications and source records attributed to I G Morris.

8 recordsLinked to original sources
PubMed1986

Transfer of passive immunity from mother to young in a teleost fish: haemagglutinating activity in the serum and eggs of plaice, Pleuronectes platessa L.

Plaice were immunized against rabbit red blood cells (rbc) and the haemagglutinating activity in their sera and eggs was isolated by gel filtration in Sephacryl S300 SF. A "heavy" (greater than 6.69 X 10(5) Daltons) and a "light" (less than 2.32 X 10(5) Daltons) component of haemagglutinating activity were present in plaice immune serum and eggs. The "heavy" haemagglutinating component had the characteristics of immunoglobulin M (IgM) antibody. By contrast the "light" component was suppressed greatly by monosaccharides but not by 2-mercaptoethanol (2-ME) and it was not precipitable against heterologous rabbit anti-plaice Ig immune serum. Since this "light" component was also identified in the serum and eggs of non-immunized plaice the activity was attributed to non-specific lectin-like activity. The presence of an inducible, high molecular weight, haemagglutinating Ig-like protein in plaice serum and eggs suggests the possibility of transfer of passive adaptive immunity from mother to young in this fish.

Animals↗
PubMed1980

Transmission of circulating IgG into preovulatory mouse eggs.

Mice were injected intravenously with radioactivity labelled rat IgG or human serum albumin (controls) and their ovaries subsequently investigated by autoradiography at the light and electron microscopical levels. Both of the administered proteins reached the cytoplasm and nuclei of the oocytes at all except the late secondary and early tertiary follicular stages. The entry of the IgG into the eggs was higher than the entry of the albumin.

Animals↗
PubMed1980

An immunofluorescence study of IgG receptors in rodent enterocytes.

Locations of IgG receptors in the small intestine of suckling rats were visualized by treating sections of the gut with IgG and FITC-labelled anti-IgG antibodies. Receptors, operative optimally at acid pH, occurred only in younger rats whose intestines were still permeable to antibodies; they were located on the brush borders of the enterocytes on the apical parts of the villi in the duodenum and jejunum. Premature disappearance of the receptors from the gut was achieved by cortisone treatment. In rats and mice, IgG from different species each competed for attachment to the receptors in vitro in the same way as it inhibited the transmission of other IgG across the intestine in vivo, thus reflecting the involvement of the receptors in the transport mechanism.

Aging↗
PubMed1979

The effect of anti-microvillus membrane antibodies on the transport of IgG across the suckling rat intestine.

Rabbits were immunized with a preparation of microvillus membranes derived from the absorptive cells of the small intestine of suckling rats. The resulting immune serum contained antibodies which reacted specifically with the brush borders and walls of cytoplasmic vesicles of the intestinal cells, and was more effective than normal serum in preventing the transport of IgG across the intestinal cells. These results reflect the participation of microvillus membrane components, or receptors, in the transport mechanism.

Animals↗