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I Gogov

Publications and source records attributed to I Gogov.

23 records · Page 2Linked to original sources

[Finding of the staphyloccoi and streptococci of mastitis on the udder after various ways and means of cleaning].

The presence of mastitis staphylococci and streptococci on the udder's surface of 400 cows was studied following application of various ways and means of cleaning. The presence of agents causing mastitis was determined before and after cleaning the udder. On uncleaned udder surfaces presence of mastitis staphylococci was observed in 52.5% and of mastitis streptococci--39.5% of udders studied. After washing with water mastitis staphylococci--on 12-20% of the udders. Following udder cleaning with solutions of antigerm 50 (0.04%) or yozan CCT (4%), mastitis staphylococci were found on 4% and 6% of the udders, respectively, while mastitis staphylococci--on 2% and 6%, respectively. Udder surface staphylococci belong in 2.2% to II phagous group, in 46.6%--to the III group, and in 37.7% -- to mixed groups. The most frequently encountered phagous types were 6 and 42D.

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[Phage relationships of coagulase-positive staphylococci isolated from raw and pasteurized cow's milk].

Studied is the behaviour to phages of 136 strains of coagulase positive cstaphylococci, isolated from raw (82), pasteurized milk stored in tanks (30) and pasteurized milk in bottles (24). The Staphylococcus aureus strains investigated are sensitive to bovine phages and, above all, to those of the 4th group (102, 107, 117 and 42D) or in combination with them. No lyses are established in the 1st and 3rd phage groups. The 107/117 and 107/117/42D are the phage types most frequently identified. The same phage types are found in both raw and pasteurized cow milk. It is established that 82 per cent of the Staphylococcus aureus strains studied with the rystal-violet test belong to types A and B after Meyer.

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[Presence of E. coli bacteria in raw and pasteurized cow's milk].

Investigated for the presence of E. coli-bacteria belonging to the O-groups of dispeptic strains, are 85 samples of raw and 212 pasteurized milk, taken from subsequent departments of the milk-producing industry. Dispeptic E. coli are detected in 11.76 per cent of the samples of raw milk studied, and in 5.18 per cent in those of the pasteurized milk. No coli forms are established in samples, taken directly from the pasteurizing equipment. The secondary infection with E. coli results from the use of vessels and equipment in the course of the technological process. E. coli-bacteria, belonging to the O-groups of dispeptic strains, are demonstrated in samples both with the aid of a coli-titre, in conformity with standard requirements, and in samples that are not standard, according to this indice. The 21 strains of E. coli-bacteria isolated are referred to the following O-groups: 06, 025, 026, 055, 078, 086, 0111, 0119, 0124, 0125 and 0127. Some of the strains isolated manifest an atypical behaviour to lactose and sucrose in comparison with the properties, indicated in the scheme by Kauffmann.

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[Coliform bacteria in raw and pasteurized milk].

Studied were 360 samples of raw and 1404 samples of pasteurized milk, collected from three milk centers, for the presence of coliform bacteria. It was found that the coli titer of the raw milk varied from 10(-5) up to 10(-7), depending on the season. The regimes of pasteurization applied kill 100 per cent of the present coli organisms. After the thermic treatment the milk was additionally contaminated with coli forms from the containers and the equipment in dependence on the conditions of washing and disinfection. The coli titer of the pasteurized milk varied from 1 to 10(-3). A total of 602 strains of coliform bacteria were isolated from the pasteurized and the raw milk; the bacteria were differentiated by the scheme of Kauffmann. Most commonly encountered were the coli forms of the following genera:Citrobacter (35 per cent), Enterobacter (29.8 per cent), Klebsiella (23.9 per cent), and Escherichia (11.3 per cent). The following species were prevailing in raw milk: Kl. aerogenes, Ent. aerogenes, Ent. cloacae, C. freundi, and C. intermedium. In pasteurized milk dominating were Kl. aerogenes, C. freundi. Ent. aerogenes, and Ent. cloacae.

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[Study of enterococci in milk].

Studied were a total of 270 samples of raw and pasteurized milk taken during the various technologic states of pasteurized mild produced for consumption at the State Economic Enterprise "Serdica", establishing the enterococcus and coli titers. It was found that the enterococcus titer of the raw milk varies from 10(-3) up to 10(-6), and the titer of the pasteurized milk at the enterprise and upon delivery through its commercial channels is from 1 up to 10(-3). The enterococcus titer of milk that was pasteurized and was kept for 24 hours at 4-5 degrees C was found to range from 1 to 10(-3), and of milk kept at 18-22 degrees C --from (0(-1) to 10(-6). Full coincidence of the enterococcus titer with the values of the coli titer was found only for pasteurized milk kept under refrigerator conditions. Along with the coli titer the enterococcus one may be used as a subsidiary means in a more strict bacteriologic control, however, it may not be referred to as the only criterion replacing the coli values in the hygiene evaluation of milk. All species of enterococci in varying counts are established in both raw and pasteurized milk. Prevailing are the strains of the Str. faecium group -- 43.2 per cent, and those of the Str. faecalis group -- 24.6 per cent.

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