PubMed Health⌕ Search

Biomedical subjects

I Gustavsson

Publications and source records attributed to I Gustavsson.

At least 55 records · Page 3Linked to original sources
PubMed1994

Regional localization of the bovine interleukin-2 (IL2) gene to chromosome 17q22-->q23 by in situ hybridization.

The interleukin-2 (IL2) gene was localized to the q22-->q23 bands of chromosome 17 in cattle using radioactive in situ hybridization. The localization confirms the recent provisional assignment of syntenic group U23 to this bovine chromosome. Increasing evidence for chromosomal banding and gene location homology within the Bovidae suggests that the IL2 gene most likely also maps to chromosome 17 in sheep, goats and buffaloes.

Animals↗
PubMed1994

Precise localization of the genes for glucose phosphate isomerase (GPI), calcium release channel (CRC), hormone-sensitive lipase (LIPE), and growth hormone (GH) in pigs, using nonradioactive in situ hybridization.

Fluorescence in situ hybridization (FISH) was applied, using genomic DNA clones, to precisely localize the genes for GPI, CRC, LIPE, and GH on pig chromosomes. The porcine CRC gene was localized to band 6q12 using both genomic and cDNA clones. The GPI and LIPE genes, which are closely linked to the CRC gene, were also mapped to the same band (6q12), using genomic lambda clones. The mapping data are a refinement of earlier findings, wherein radioactive in situ hybridization was used and the assignments included both the short and long arms. Results of the present study clearly exclude the short arm as the location for the three genes. Further, using a genomic cosmid clone, the GH gene was mapped to band 12p14. Compared to the earlier assignments, which included almost the entire short arm of the chromosome due to the use of radioactive in situ hybridization, the present FISH findings provide a band-specific localization for the gene. A modified, simpler version of the posthybridization trypsin/EDTA banding method is also presented.

Animals↗
PubMed1993

Assignment of 20 microsatellite markers to the porcine linkage map.

Twenty-one porcine microsatellite markers were developed by screening DNA libraries and by a computer search of databases. The microsatellites were typed in a large three-generation family established by a cross between the European wild pig and a Swedish Yorkshire breed. Linkage analysis benefited from the fact that due to the divergence between the parental populations, the degree of microsatellite polymorphism was significantly higher in the F1 animals than in either of the parental populations. Parallel typing of a set of 35 restriction fragment length polymorphism, protein, and blood group markers rendered it possible to assign as many as 20 of the microsatellites to the porcine linkage map. Fourteen microsatellites were localized to a chromosome segment, whereas six constituted parts of unassigned linkage groups. Analysis of four microsatellites within genes allowed the assignment of the endoplasmic reticulum Ca2+ transport ATPase locus to chromosome 14, the assignment of the interferon-gamma and the diacylglycerol kinase loci to a new linkage group (XI), and the localization of the tumor necrosis factor beta locus close to the major histocompatibility complex (SLA) on chromosome 7 to be confirmed. Fluorescence in situ hybridization mapping of two microsatellite-containing cosmids assigned two linkage groups to chromosomes 9 and 12, respectively. In total, 27 new markers were added to the porcine linkage map, thereby almost doubling the number of markers on the map. Linkage groups are now present on 10 of 18 of the pig autosomes.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1993

Assignment of the linkage group EAM-TYRP2-TPP2 to chromosome 11 in pigs by in situ hybridization mapping of the TPP2 gene.

Restriction fragment length polymorphisms are described for the genes coding for tripeptidyl peptidase II (TPP2) and tyrosinase related protein II (TYRP2) in pigs. A linkage group comprising these loci and the locus for blood group M (EAM) was established by two-point lod score analysis in a three-generation pedigree. Multipoint analysis indicated the linear order EAM-1.1-TYRP2-8.4-TPP2 (recombination distances are given as Kosambi cM). The linkage group was assigned to porcine chromosome 11--the first on this chromosome--through in situ hybridization mapping of the TPP2 gene. TPP2 is the first gene localized on this chromosome using in situ hybridization.

Aminopeptidases↗
PubMed1993

In situ hybridization mapping and restriction fragment length polymorphism analysis of the porcine albumin (ALB) and transferrin (TF) genes.

In situ hybridization analyses were conducted on porcine metaphase chromosomes using porcine liver albumin (ALB) and transferrin (TF) cDNA probes. The ALB gene was assigned to the q12 band of chromosome 8 and the TF gene to the q31 band of chromosome 13. For the latter, a statistically significant secondary peak was observed on the 6p15 band. However, the TF probe predominantly hybridized to the 13q31 band, indicating that this band is the most likely site of the TF gene. Since the TF gene belongs to linkage group V, this linkage group can now be assigned to chromosome 13. The TF and ALB probes were also used for restriction fragment length polymorphism (RFLP) analysis. A screening of 10 unrelated animals revealed TaqI RFLPs for both ALB and TF. Family studies indicated that the ALB and TF polymorphisms were controlled by three and two alleles, respectively.

Alleles↗
PubMed1993

Localization of the ceruloplasmin (CP) gene to the q32-q33 bands of chromosome 13 in pigs by in situ hybridization.

Ceruloplasmin (CP) is a copper-binding protein in vertebrate plasma. In the present study, the porcine ceruloplasmin gene was localized to the 13q32-q33 bands by in situ hybridization, using a human CP cDNA probe. This confirmed the localization of the porcine linkage group V to chromosome 13. The CP locus is closely linked to the transferrin locus in pigs. Their relative physical order on chromosome 13 is discussed. Comparisons are made with the order of these two loci in other mammalian species.

Animals↗
PubMed1993

The gene for bovine interferon gamma (IFNG) maps to the q22-q24 bands of chromosome 5 in cattle.

A approximately 700 bp bovine IFNG cDNA probe was used for the chromosomal localization of the IFNG gene in cattle using in situ hybridization technique. This locus has previously been assigned to chromosome 5 by analysis of a somatic cell hybrid panel. The in situ hybridization results obtained in the present study allow us to map the locus at the 5q22-q24 bands. Relative location of the IFNG gene and the class II cytokeratin and HOX3 gene clusters in cattle and humans is discussed.

Animals↗
PubMed1993

Meiotic chromosome asynapsis in a boar with a reciprocal translocation and acquired testicular degeneration.

During a period of performance testing, a boar used for artificial insemination purposes was found responsible for reduced litter size and slightly increased incidence of repeat breeding. The qualitative and quantitative semen characteristics, however, were within normal limits. Later, evaluation of the semen during a period of 3 months revealed a decreased sperm concentration, but sperm cell morphology and mobility were normal. At castration, the boar, which had suffered from scrotal inflammation, was found to have hypoplastic gonads, the right testis being smaller than the left one. Histologically there was spermatogenic arrest at the primary spermatocyte level in almost all tubules of the right testis. In the left testis, histology was more heterogeneous, with some tubules containing all developmental stages, while others had an almost complete spermatogenic arrest. Cytogenetically the boar was carrying a translocation, rcp(2;14)(p14;q23). Synaptonemal complex analysis revealed complete quadrivalent pairing in 21 out of 43 cells analysed. The remaining cells demonstrated quadrivalents with axes moderately or extensively unpaired. There was a distinct difference between cells from the right vs. the left testis. The latter cells showed more completely paired translocation configurations. Moreover, in 14 and 7 cells analysed from the right and left testis, respectively, the translocation configuration could not be identified. This was due to chromosome asynapsis and frequent occurrence of gaps in the chromosomes, including others than those of the translocation, plus an abundant cellular deposit which formed a dense cell background. Association or pairing of the quadrivalent with the sex bivalent was seen in one cell only. At diakinesis-MI, most cells had a ring-shaped quadrivalent. It is believed that the asynapsis of chromosomes during the prophase of meiosis was to a large extent due to the degeneration caused by the inflammation processes.

Animals↗
PubMed1993

Synaptonemal complex analysis of a reciprocal translocation, rcp(20;24) (q17;q25), in a subfertile bull.

A reciprocal translocation was identified in a subfertile artificial insemination bull. Somatic chromosome investigation of G-banded metaphases demonstrated a 60,XY,rcp(20;24)(q17;q25) karyotype for the carrier. Synaptonemal complex analysis of the translocation by electron microscopy revealed an irregular pairing behavior of the chromosome axes involved, which resulted in a variety of configurations at pachytene. Not only was the expected quadrivalent configuration present, but also a trivalent plus univalent and two heteromorphic bivalents. Most common was an incompletely or completely paired quadrivalent configuration, which was non-homologously paired. XY association with the multivalent was seen only rarely. Histological analysis of testicular tissue showed meiotic arrest in some tubules. However, the semen picture was normal.

Animals↗
PubMed1992

Embryo quality and andrological study of two subfertile bulls versus five control bulls with normal fertility.

Andrological studies and embryo morphology evaluation of superovulated cows were performed on 2 randomly selected subfertile dairy bulls whose semen was used for artificial insemination and on 5 control bulls with normal fertility. Neither sperm motility studies, nor sperm morphology or testicular measurements differed between the subfertile and the control bulls. Altogether 315 ova were recovered from 41 superovulated cows inseminated with semen collected from either the subfertile or the normal control bulls. The spermatozoa of one of the 2 subfertile bulls was shown to have a decreased ability to fertilize superovulated ova, while the other subfertile animal, the bull with the lowest no-return rate, was found by chromosome analysis to have a reciprocal translocation (60, XY, rcp 20:24), causing embryonic death. We suggest that subfertile bulls should not be used in commercial embryo transfer programs nor in artificial insemination and that andrological studies on subfertile bulls with good sperm motility should include evaluation of 6- to 7-day-old ova from superovulated cows to determine if the fertilization rate is normal or impaired. A chromosome analysis should also be performed when a subjertile bull has a normal fertilization rate of ova.

Journal Article↗
PubMed1992

Stillborns, partially monosomic and partially trisomic, in the offspring of a boar carrying a translocation: rcp(14;15)(q29;q24).

Smaller liters and an increased rate of stillbirth were observed among the offspring of a boar carrying a reciprocal translocation, rcp(14;15)(q29;q24). The translocation appeared to be of de novo origin since, according to the owner, the sire and dam of the proband boar both produced normal-sized litters and no stillbirths. The increased rate of stillbirth among offspring of the proband was due to the production of zygotes partially monosomic for chromosome 14 (14q29-qter) and partially trisomic for chromosome 15 (15q24----qter). Three out of 4 such stillborn piglets had a cleft palate. One of the 4 also had a cardiac septal defect. Two partially monosomic and partially trisomic foetuses recovered at 95 days' pregnancy appeared patho-anatomically normal. The smaller litters were assumed to be due to an early wastage of embryos having other types of unbalanced karyotypes caused by the reciprocal translocation.

Animals↗
PubMed1992

Localization of the citrate synthase (CS) gene to the p12-p13 bands of chromosome 5 in pigs by in situ hybridization.

Citrate synthase (CS) is a key enzyme of the Krebs tricarboxylic acid cycle. A 1.4 kb porcine CS cDNA probe was used to chromosomally localize the CS gene in pigs by in situ hybridization. Two in situ hybridization experiments were conducted. Although the first experiment indicated a distinct signal on the 5p12-p13 bands, a secondary signal was observed on the 13q24-q32 bands. Hence, a second in situ hybridization experiment was conducted at higher stringency. The results demonstrated a consistent signal on the 5p12-p13 bands, and the signal on chromosome 13 was scattered with no prominent secondary peak. The CS gene was therefore assigned to the p12-p13 bands of chromosome 5 in pigs.

Animals↗
PubMed1992

Chromosomal localization of the hormone sensitive lipase (LIPE) and insulin receptor (INSR) genes in pigs.

Using rat hormone sensitive lipase (LIPE) and human insulin receptor (INSR) cDNA probes, the LIPE gene was assigned to chromosome 6p11-q21 and the INSR gene to chromosome 2q11-q21 in pigs by in situ hybridization. In humans, these two genes are located on the q and p arms of chromosome 19, respectively. The present results provide the first in situ hybridization mapping data for porcine chromosome 2.

Animals↗
PubMed1992

Assignment of the bovine immunoglobulin gamma heavy chain (IGHG) gene to chromosome 21q24 by in situ hybridization.

By the use of in situ hybridization, the bovine immunoglobulin gamma heavy chain (IGHG) gene was assigned to chromosome 21q24. Fifteen percent of the total grains were scored on chromosome 21, with about 65 percent of these grains located on the q24 band. The present results confirm a previous synteny study and provide the precise chromosomal localization of this gene in the cattle genome.

Animals↗