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Biomedical subjects

I H Ansari

Publications and source records attributed to I H Ansari.

16 recordsLinked to original sources
PubMed1995

Protracted viremia during acute sporadic hepatitis E virus infection.

BACKGROUND/AIMS: Hepatitis E virus (HEV) is associated with epidemic and sporadic hepatitis in developing countries. The disease is largely self-limited with no long-term sequelae. The source of HEV for maintenance of the disease in an endemic area is unknown. This study investigated the occurrence and duration of viremia in patients with acute sporadic HEV infection. METHODS: In 26 of 37 patients with sporadic acute non-A, non-B viral hepatitis, HEV infection was diagnosed based on positivity for immunoglobulin M anti-HEV and/or presence of viremia as shown by reverse-transcription polymerase chain reaction. In 4 patients, fecal samples were analyzed for presence of virus using polymerase chain reaction. Multiple samples were studied at varying times in 20 patients. RESULTS: Viremia was detected in 19 of 26 patients. Two patients had viremia in the absence of immunoglobulin M anti-HEV. Four patients had protracted viremia of 45-112 days' duration. One patient showed fecal virus shedding up to the 52nd day of illness. CONCLUSIONS: Protracted viremia and prolonged fecal shedding of HEV were shown in a small group of patients. These patients may serve as temporary virus carriers responsible for continuous contamination of the sewage water.

Acute Disease↗
PubMed1995

An Indian strain of hepatitis E virus (HEV): cloning, sequence, and expression of structural region and antibody responses in sera from individuals from an area of high-level HEV endemicity.

Hepatitis E virus (HEV) is responsible for a majority of sporadic and epidemic viral hepatitides in India and other developing countries. Even though the genomes of four geographically distinct strains of HEV have been cloned and sequenced, the Indian strain of HEV remains largely uncharacterized. We have cloned and sequenced about 2.2 kb of the HEV genome constituting the structural region from an Indian strain of HEV. The nucleotide and amino acid sequences show a high degree of conservation with sequences from other HEV strains. Open reading frames (ORF) 2 and 3 were expressed in Escherichia coli as N-terminal hexahistidine epitope fusions. The purified proteins were then used in an immunoblot assay to evaluate the antibody status in sera from individuals from an area of high-level HEV endemicity. The anti-ORF2 antibodies were found to be nonspecific and could not be correlated to clinical disease. The immunoglobulin M anti-ORF3 was found to be specific for the presence of acute disease. The implications of these findings in HEV diagnosis and vaccine development are discussed.

Amino Acid Sequence↗
PubMed1994

Making a custom tray for elastomeric impression materials without a primary cast.

The custom tray is recommended for impressions in which it is desirable to establish precise borders, such as Kennedy class I maxillary partial dentures, where an accurate postpalatal seal is a requirement. The peripheries, including the postpalatal seal, can be established with a high degree of accuracy with a custom tray. A procedure is described that does not require a primary impression or a primary cast to make a custom tray for elastomeric impression materials.

Dental Impression Materials↗
PubMed1994

A procedure for reorienting a cast on a surveyor.

The tilt of a cast must be recorded so that it can be easily transmitted to the dental technician, who should then be able to transfer the original tilt from the master cast directly to any necessary duplicate casts. A procedure is presented that can easily record and transfer the tilt with the help of an impression made with a custom-made U-shaped universal tray.

Dental Impression Technique↗
PubMed1994

A boxing procedure for corrected cast impressions.

This new method for boxing a corrected cast impression for a distal extension removable partial denture improves the procedure. The framework with its impression is seated on the remainder of the cast after anatomic ridges have been removed and luted with sticky wax, and the assembly is inserted into a previously formed thermoplastic box and poured to make a new corrected cast.

Acrylic Resins↗
PubMed1994

Duplicating an existing complete denture to make a replica.

Sometimes it is desirable to make a few changes when making new complete dentures for a patient who has been wearing old dentures for a long time and is satisfied with them. Replica dentures can be made for these patients, and success with the new dentures can be assured. A procedure is presented for duplication of the old dentures to make replicas.

Calcium Sulfate↗