PubMed Health⌕ Search

Biomedical subjects

I Hilgert

Publications and source records attributed to I Hilgert.

At least 55 records · Page 3Linked to original sources
PubMed1988

Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).

The specificities of several monoclonal antibodies described by us earlier were determined more exactly: (1) antibodies MEM-31 and MEM-32 are directed against CD8 and CD5 T cell antigens, respectively: (2) MEM-15 and MEM-18 react with the CD14 monocyte antigen; (3) MEM-28 recognizes the CD45 pan-leucocyte antigen. Several new monoclonal antibodies are described: (4) MEM-55 and MEM-58, reactive with subsets of CD45-related molecules; (5) MEM-43, recognizing a broadly expressed 18-kDa glycoprotein; (6) MEM-57, reacting with the CD3 antigen complex: (7) MEM-59 recognizing the CD43 leucocyte antigen; (8) MEM-74, which reacts with an unidentified antigen strongly expressed on several cell lines and many leukaemic cells but absent from most resting leucocytes; (9) MEM-75, directed against transferrin receptor, and (10) MEM-78, recognizing CD10 (CALLA) antigen.

Antibodies, Monoclonal↗
PubMed1986

Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen.

Two monoclonal antibodies, MEM-15 and MEM-18, were prepared which recognize a monocyte 53-kDa antigen. A soluble form of this antigen was found in most normal sera and in urine of some nephrotic patients. Milligram amounts of this glycoprotein were isolated by immunoaffinity chromatography from urine and used for quantitative amino acid and carbohydrate analysis and N-terminal amino acid sequencing. The reactivity of the isolated antigen with several previously described monoclonal antibodies indicates that it is the antigen previously called MY-4 or MY-23. A significant homology exists between the sequenced N-terminal portion of the antigen and sequences found in the kinase-related transforming protein src and in the precursor of epidermal growth factor.

Amino Acid Sequence↗
PubMed1986

Modification of suppressor/cytotoxic and helper subsets in lentil lectin-pretreated mice.

The effect of lentil lectin (LCA) on various lymphocyte subpopulations in the spleen, lymph nodes and thymus of CBA mice was studied. The most marked effect after five 1-mg LCA doses was observed in the spleen. LCA caused a drop in both the total and relative number of cells carrying all the markers examined, i.e., Thy 1, L3/T4a, Lyt 2, and sIg, so that large numbers of lymphocytes did not express any of them. A dramatic decrease in the Thy 1-positive cell number is mainly due to depletion of the L3/T4a (helper) T cell subpopulation, whereas the Lyt 2+ (suppressor/cytotoxic) cells are less affected. The decrease in the Thy 1+, Lyt 1+ and L3/T4a+ cell numbers in the thymus and lymph nodes is smaller, the percentage of Lyt 2+ cells is even increased after LCA treatment. Our results indicate that two mechanisms play a role in the induction of allotransplantation tolerance by LCA: 'depletion' of helper T cells and a relative increase in the regulatory (suppressor) cell ratio, which may support allograft survival.

Animals↗
PubMed1986

Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens.

Seven murine monoclonal antibodies were prepared that react with human class II antigens. Four of them (HL-39, MEM-12, MEM-24G, and MEM-32B: react with a monomorphic determinant dependent on association of heavy and light chains of DR antigens, two others (HL-38 and HL-40) recognize a monomorphic determinant localized on the light chains of DR and DP antigens. The antibody HL-37 is directed against a determinant present on DQ1 and DQ3, but not DQ2 molecules; at least in the case of DQ1, the epitope recognized is located on the light chain.

Animals↗
PubMed1986

Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen.

Monoclonal antibodies against several human leucocyte cell surface antigens were prepared and characterized: B2M-02, a non-cytotoxic antibody against beta-2-microglobulin; MEM-09 and MEM-40, against immunoglobulin kappa-type light chains; MEM-12, MEM-24G and MEM-32B, all against a monomorphic determinant in MHC class II antigens, presumably HLA-DR, dependent on the association of alpha and beta chains; MEM-15 and MEM-18, against a monocyte antigen of 55 kDa; MEM-28, against a 200 kDa antigen expressed on all leucocytes; MEM-31, against the T8 antigen of the cytotoxic/suppressor T-lymphocyte subpopulation; MEM-32, against the T1 antigen of T lymphocytes.

Animals↗
PubMed1985

The effect of H-2IJ disparity on induction of allotransplantation tolerance by lentil lectin.

The effect of an H-2IJ disparity on skin graft survival was studied in 18 mouse donor-recipient strain combinations, in which the recipients were treated with an efficient immunosuppressant, lentil lectin (LCA). The simultaneous I-J disparity essentially had no (or a slightly adverse) effect on the graft survival times in strain combinations differing at the K and I-A loci or in the entire H-2 complex. In two strain combinations incompatible at the D locus, the simultaneous I-J disparity promoted graft survival. The disparity at the I-J locus therefore seems to have only a marginal effect on the survival of allografts in most of the LCA-treated recipients, but it may promote graft survival in some animals. A similar tolerance-promoting effect was also observed with D disparity.

Animals↗
PubMed1984

Murine hybridoma monoclonal antibodies against insulin: cross-reactivity with insulins of three species and blocking of insulin binding to its receptor.

Six hybridoma cell lines secreting monoclonal antibodies against pig insulin and cross-reacting with human and bovine insulins were obtained. Five of these monoclonal antibodies were IgG1, kappa, one IgG2b, kappa; their pI values were in the range of pH 6.3-7.4 and dissociation constants of the insulin-antibody complexes were 0.3-2 X 10(-8) mol/l, as determined by an immunoradiometric inhibition assay. All of these antibodies reacted with sterically closely related determinants and blocked the binding of 125I-pig insulin to the receptor on human MOLT-4 cell line.

Animals↗
PubMed1984

The effect of lentil lectin treatment on the survival of rat skin allografts in strain combinations with different genetic disparity.

The effect of lentil lectin on the survival of skin allografts was investigated in six rat strain combinations with different genetic disparities between the donors and recipients. LCA was administered i.p. (50 mg/kg) to the recipients daily, starting with the day of transplantation (day 0) until graft rejection, except that the doses on days 1, 3, 5, 7, and 9 were given i.v. (25 mg/kg). LCA treatment was more efficient in prolongation of skin graft survival time in congenic strain combinations differing in multiple non-RT1 antigens (RT1-compatible) than in those involving RT1 disparities.

Animals↗
PubMed1984

The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin.

A murine hybridoma cell line was obtained producing monoclonal antibody specific for human beta 2-microglobulin (beta 2m). The antibody reacts strongly with cell-associted human beta 2m, weakly with the cells of the monkey Cercopithecus aethiops, but not with any other non-primate cells tested. It is of IgG2a isotype, its pI is 6.9-7.1 and dissociation constant of its complex with soluble beta 2m is K = 1.5 X 10(-8) mol/l. The antibody also binds to beta 2m after electrophoretic separation under the reducing and denaturating conditions and blotting onto nitrocellulose. Immobilization of this antibody on Sepharose 4B yields an efficient immunosorbent for purification of beta 2m.

Antibodies, Monoclonal↗
PubMed1983

Adult transplantation tolerance induced by lentil lectin. III. Induction of transplantation tolerance by lentil lectin in mouse strain combinations with different H-2 disparities: tolerogenic effect of H-2D region antigens.

The survival time of skin allografts was investigated in 28 combinations of mouse strains differing at various loci, in adult recipients treated with lentil seed lectin (LCA). The recipients were given 1 mg of LCA daily (i.v. or i.p.) after transplantation of the skin graft. The immunosuppressive effect of LCA was generally inversely related to the conventional strength of the allotransplantation reaction, i.e. the rate of rejection of allografts on untreated recipients. In the case of H-2D antigenic disparities, the grafts usually survived for more than 100 days. The H-2A or H-2K disparity appeared to be relatively resistant to induction of tolerance by LCA treatment. The relative resistance of various H-2 antigens toward the induction of tolerance by LCA differs to some extent from that observed in experiments on the induction of neonatal tolerance by semiallogeneic lymphocytes as described in literature.

Animals↗
PubMed1983

Persistence of regulation mechanism responsible for inhibition of allotransplantation reactions in the absence of alloantigen.

The purpose of the present work was to examine the persistence of regulatory cells implicated in the inhibition of allotransplantation reactions in recipient mice in the absence of antigenic material. Two experimental systems were used: (1) When neonatally induced tolerance (obtained as a result of cell-free spleen extract administration in the B10.D2 versus M504 strain combination) was adoptively transferred, the persistence of suppressor cells was detected in secondary recipients receiving a second homograft as long as 30 days after removal of a first homograft to the tolerance inducing haplotype. The removal of the skin homograft presenting a source of antigen for 60 days, resulted in tolerance interruption when a second test graft was applied. (2) When tolerance was induced in CBA mice with spleen-cell antigenic extracts and CFA and spleen cells from such animals were transferred to secondary recipients the following results were observed: Secondary recipients grafted with SaI tumour cells derived from the tolerance inducing A strain 20 days after tolerance induction presented an enhanced tumour growth. The adoptive transfer of this effect was at least partly due to IJ+ lymphocytes, since their removal reduced adoptive enhancement. However, if the cell transfer was delayed for 56 days after tolerance induction, the depletion of IJ+ or Thy 1.2+ cells did not affect the capacity of spleen cells to cause an enhanced growth of the homografted SaI cells. A delay of 100 days following donor enhancement induction did not result any adoptive transfer of SaI enhancement.

Animals↗
PubMed1983

Adult transplantation tolerance induced by lentil lectin. I. The fate of tolerogenic lentil seed lectin after injection into mice.

Intravenous administration of lentil lectin is more efficient in the induction of transplantation tolerance to mouse skin allograft than intraperitoneal or subcutaneous administration. 131I-labelled LCA is eliminated relatively rapidly from the mouse body after i.v. injection (approximately 80% of a 1 mg dose eliminated after 48 h); LCA administered i.p. or s.c. is eliminated 2-3 times more slowly. The rate of elimination of LCA administered i.v. is only slightly lower than that of bovine serum albumin. Tolerogenic treatment by multiple i.p. or i.p. + i.v. doses of LCA only elicited a relatively low level of anti-LCA antibodies in the sera of recipients. Both LCA and BSA were distributed relatively homogeneously throughout the body but increased amounts of LCA were accumulated in the spleen, lymph nodes and lung.

Animals↗
PubMed1981

On the influence of hydrocortisone on the stimulation of mouse spleen cells by PHA. Evidence for the involvement of suppressor cells.

The stimulation rate with PHA of murine spleen cells increases after removal of glass-adherent cells from the total spleen population. Unfractionated spleen population from HC treated mice suppresses the response to PHA of glass non-adherent cells from untreated animals only 24-48 h after HC treatment. From the third day on after the treatment the suppressive effect disappears.

Animals↗