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Biomedical subjects

I I Benediktov

Publications and source records attributed to I I Benediktov.

At least 19 recordsLinked to original sources
PubMed2001

[Laboratory diagnosis of toxoplasmosis using polymerase chain reaction].

Polymorphism of clinical manifestations in Toxoplasma infection and variegated disease patterns virtually rule out the diagnosis based solely on clinical symptoms, which makes modern laboratory tests particularly important. Amplification test system based on the polymerase chain reaction has been developed for the diagnosis of Toxoplasma infection. Computer analysis of nucleotide sequence of Toxoplasma gondii surface antigen gene P30 was analyzed, which helped choose and synthesize specific oligonucleotide primers. A method for biological material processing was selected, allowing sufficient DNA output. Optimal conditions for amplification reaction, ensuring absolute specificity and high (10-100 cells/sample) sensitivity, were determined.

Animals↗
PubMed1991

[Phasic changes in the functional activity of alpha2- and beta2- subtypes of platelet adrenergic receptors--objective criteria of adrenoreactive imbalance in the evaluation of severity of nephropathies in pregnancy].

The pregnant suffering from nephropathies were found to have autonomic nervous control disorders closely related to the major platelet vascular and plasma hemostatic, hemodynamic, and microcirculatory parameters. A combined examination of the macro- and ++micro-hemodynamics and status of adrenoreactive structures assessed the adaptation of a pregnant to nephropathy and her reserve potentialities. Adrenoreactive structure derangement is a measure of the severity of nephropathy. Abnormalities along the whole vasculature are characteristic of dysadaptation.

Blood Platelets↗
PubMed1987

[Hormone and lipid interrelations in young women with normal and disturbed balance of sex steroids].

Altogether 121 patients with sclerocystic ovaries and 40 normal women aged 16 to 35 years were examined for excretion of estrogens, androgens and pregnandiol with urine and for the blood content of total cholesterol (TC), triglycerides (TG) and high density lipoprotein cholesterol (HDLC) followed by hyperlipoproteinemia (HLP) phenotyping. In the group of normal women, low production of estrogens and pregnandiol was coupled with a reduction in the content of HDLC and an increase in that of TG. In the patients, no relationship was established between the changes in the concentration of estrogens and pregnandiol and the lipid content. Meanwhile the androgen content, which was higher as compared with that in the normal women, correlated with the rise of the TG and TC level, accompanied by a noticeable subsidence of the HDLC level. As compared with the normal persons, the atherogenic types of HLP in the patients occurred 2.2 times as frequently. The data obtained indicate an important role of the primary imbalance of sex steroids in the development of dyslipoproteinemia in young women.

Adolescent↗
PubMed1984

[Cardiovascular disorders, autonomic disorders and atherogenic dyslipoproteinemias in young women with the sclerotic ovary syndrome].

The authors studied 158 young women with the syndrome of sclerocystic ovaries (SCO) and 40 healthy subjects. The patients showed a high rate of vegetovascular dystonia, predominantly according to the hyperparasympathicotonic type, cardialgia and dyspnea. In 54.5% of the patients lipid metabolism disturbances of the atherogenic nature were elicited, being most pronounced in the hypothalamic-pituitary form of the disease. It is postulated that in cases of the SCO syndrome, the deficit of estrogens and pregnanediol decreases "immunity" of females to the development of atherogenic dyslipoproteinemias while a relative or absolute hyperandrogenia accounts for the transformation of the "female" type of the lipid spectrum into the "male" type with lower levels of cholesterol of high density lipoproteins (LP) and higher levels of TG, and cholesterol of very low density LP and of low density LP. It is recommended that the SCO syndrome should be included into the risk factors for atherosclerosis and coronary heart disease.

Adolescent↗