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Biomedical subjects

I I Hamdan

Publications and source records attributed to I I Hamdan.

7 recordsLinked to original sources
PubMed2004

Studies on the in vitro and in vivo hypoglycemic activities of some medicinal plants used in treatment of diabetes in Jordanian traditional medicine.

Ferula persica, Paronychia argentea, and Pistacia atlantica are three of the plants widely recommended by the herbalists and used for their hypoglycemic activity in Jordan. Aqueous extracts of these plants were tested in vitro for their alpha amylase inhibitory activity and in vivo for their hypoglycemic activity in normoglycemic and streptozocin-induced hyperglycemic rats. Although the three plants were advocated for their hypoglycemic effects in Jordanian traditional herbal medicine; none of them showed significant hypoglycemic activity compared to the untreated animals. Paronychia argentea and Pistacia atlantica showed significant alpha amylase inhibitory activity while Ferula persica did not demonstrate any alpha amylase inhibitory activity. The main conclusion of this work was the concern over the unjustified claims of the uses of some herbal medicine in Jordan and possibly in other countries.

Animals↗
PubMed2004

In vitro alpha amylase inhibitory effect of some clinically-used drugs.

Twenty six medicinal compounds used for treating various diseases and representing different chemical and pharmacological classes were tested for their potential alpha amylase inhibitory activity in vitro. Three of these (tetracycline, enalapril and captopril) were found to have a substantial alpha amylase inhibitory activity. The effect was shown to be dose dependent and the IC50 was determined (tetracycline = 0.59 mM, enalapril = 0.29 mM, and captopril = 0.78 mM) and compared to that of acarbose (0.0062 = mM). The potential pharmacological implications of this effect are discussed.

Algorithms↗
PubMed2001

In vitro study of the interaction between omeprazole and the metal ions Zn(II), Cu(II), and Co(II).

The purpose of this study was to investigate the possibility of a chemical interaction between omeprazole (OM) and the metal ions Zn(II), Cu(II), and Co(II). Using UV absorption spectroscopy and elemental analysis, it was demonstrated that all of the studied metals form complexes with OM. The spectral changes associated with the complexation reaction were used to obtain the stoichiometry and formation constants of the complexes. In all cases complexes were found to form in 1:2 metal to OM ratio. In the case of cobalt another complex species which appeared as a green precipitate was also evident. Copper was shown to form the complex with the formula Cu3(OM)2 in addition to Cu(OM)2. The complexation of cobalt and copper to OM was found to be time dependent and the time required for the completion of the reaction was determined (about 6 h). Apparent binding constants were also determined.

Anti-Ulcer Agents↗
PubMed1998

Use of capillary electrophoresis in the study of ligand-DNA interactions.

Free solution capillary electrophoresis (FSCE) has been used to separate two non-self-complementary 12mer oligonucleotide duplexes: d(AAATTATATTAT).d(ATAA-TATAATTT) and d(GGGCCGCGCCGC).d(GCGGCGCGGCCC). Titration of mixtures of the two oligonucleotides with model intercalators (ethidium bromide andactinomycin D) and minor groove binders (netropsin, Hoechst 33258 and distamycin) has shown the suitability of FSCE as a method to study the sequence selectivity of DNA binding agents. Binding data have shown cooperativity of binding for netropsin and Hoechst 33258 and have provided ligand:DNA binding ratios for all five compounds. Cooperativity of netropsin binding to a 12mer with two potential sites has been demonstrated for the first time. Ligands binding in the minor groove caused changes in migration time and peak shape which were significantly different from those caused by intercalators.

Binding, Competitive↗
PubMed1998

Separation of pd(GC)12 from pd(AT)12 by free solution capillary electrophoresis.

The two synthetic self complementary oligonucleotides pd(AT)12 and pd(GC)12 were separated by free solution capillary electrophoresis (CZE) using simple borate buffers. The effects of pH (7.5-9) and the concentration of the buffer (0.03-0.35 M) were investigated. Higher pH values and buffer concentrations led to better resolution and longer migration times, the pH having a more pronounced effect on the separation than the concentration of the buffer. It is proposed that the conformation and effective length of the oligonucleotides may have a role in their separation in free solution capillary electrophoresis.

Boric Acids↗