Biomedical subjects

I Ia Uchitel'

Publications and source records attributed to I Ia Uchitel'.

16 recordsLinked to original sources

PubMed1980

[Macrophage activation by blastolysin].

Blastolysin consisting largely of glycopeptide fragments of cell walls from Lactobacillus bulgaricus has a significant antineoplastic activity against a number of transplanted and spontaneous animal tumours. Blastolysin exerts a pronounced adjuvant action and activates the cells of the mononuclear phagocytic system. This is shown by stimulation of phagocytosis and digestion as well as by acquired ability of macrophages to elicit a cytotoxic action on allogeneic and syngeneic target cells (labelled with radioactive chromium) with abnormal growth. Administration of blastolysin or BCG was demonstrated to lead to variations in the time course of macrophage acquired cytotoxicity. Macrophage activation induced by blastolysin has a clearly pronounced two-phasic nature. During the first short phase the cytotoxicity is seen 2 hours following blastolysin administration, rapidly disappears and emerges after 2 weeks, remaining unchanged up to the 5th week (experimental period).

Animals↗

PubMed1977

[Limits of the phagocytizing capability of macrophages].

The capacity of macrophage peritoneal cells to ingest both different doses of the antigen (sheep red blood cells--SRBC) and the antigens differing in their physico-chemical properties (SRBC, rat red blood cells, typhoid vaccine) was studied. A manifold increase in the SRBC dose failed to influence the amount of the antigen ingestion in the course of a definite time period. When mice macrophage cells were obtained at short periods after the injection of red blood cells of different animal species, there was a decrease of the capacity of these cells to the red blood cells ingestion in vitro. Preincubation of macrophages in vivo or in vitro with all the antigens tested increased sharply their capacity to phagocytize the typhoid vaccine.

Animals↗

PubMed1976

[General regularities of the macrophage reaction in the administration of various antigens and the phagocytosis of microorganisms].

A study of ingestion and elimination of cells of peritoneal exudate (CPE) of mouse labeled antigens of various physico-chemical nature with a simultaneous analysis of their influence on the function of the enzymatic systems of macrophages showed that both the corpuscular (sheep erythrocytes, typhoid vaccine) and the soluble (albumin, endotoxin of S. typhi, tetanus and staphylococcus toxoid) antigens caused a unitypical reaction of the cells of monocytic phagocytic system. Thirty minutes after the administration the principal mass of labeled antigens (albumin, typhoid vaccine, sheep erythrocytes) was phagocytized by macrophages and was revealed chiefly in their phagolysosomal fraction. The greater part of radioactive material was eliminated in the course of the first 24 hours; however, some of it could be found in the macrophages for a long time. During the process of phagocytosis the activity of lysosomal (catepsin, acid phosphatase, desoxyribonuclease, beta-glucoronidase) enzymes in the macrophages decreased and the activity of redox (succinic dehydrogenase, NAD-N2-diaphorase) enzymes became intensified. A fall of catepsin activity in the CPE of mice 30 minutes after the intraperitoneal administration of the antigens was accompanied by its activation in the cells of the spleen.

Animals↗

PubMed1975

[The influence of anterior and posterior hypothalamic structures on the engulfment and digestion of an antigen by macrophages and on India ink clearance].

Bilateral electrolytic coagulation of the posterior and supraoptic hypothalamic nuclei of rabbits had practically no effect on the engulfment of sheep erythrocytes labeled with radioactive sodium chromate retarding chiefly the rate of the antigen catabolism and promoting its retention in the subcellular fractions of the macrophages function than injury of the anterior one. Coagulation of the supraoptic nucleus accelerated India ink clearance at all the periods of investigation, whereas injury of the posterior hypothalamus failed to influence this process.

Animals↗

PubMed1975

[Effect of adjuvants on the cellular function of the monocytic phagocytosing system].

The authors studied the effect of adjuvants differing by origin and physico-chemical nature (complete Freund's adjuvant, S. typhi endoxin, cadmium sulfate, iron trichloride) on the ingestion and digestion of erythrocytes of the sheep by the cells of monocytic phagocytizing system, on the persistence of the antigen in these cells, its complexation with the RNA-macrophages and the function of their lysosomal apparatus. The adjuvants change the phagocytizing capacity of the macrophages only in their administration in vivo. Administration to the animals of Freund adjuvant and of the S. typhic endotoxin somewhat increased the ingestion of the antigens, whereas the administration of FeCl3 and CdSO4 failed to change it or even somewhat decreased it. The capacity of ingestion of the antigen in vitro in macrophages obtained from the animals treated with the adjuvants was changed in comparison with the normal. All the adjuvants tested produced a marked action on the lysosomal apparatus of the cells of the monocytic phagocytizing system: they changed the activity of catepsin, promoted the accumulation and the retention in the lysosomes of the highly immunogenic fractions of the antigen, and increased the permeability (except the CdSO4 of the lysosomal membranes in the cells of the antigen binding with the RNA of the cells of the peritoneal exudate or the splenic cells.

Adjuvants, Immunologic↗

PubMed1975