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Biomedical subjects

I Ihse

Publications and source records attributed to I Ihse.

At least 91 records · Page 5Linked to original sources
PubMed1992

Endogenous hypercholecystokininemia model in the hamster: trophic effect on the exocrine pancreas.

Using Syrian golden hamsters, we studied the effect of pancreaticobiliary diversion (PBD) on plasma cholecystokinin (CCK) and exocrine pancreatic tissue over 5, 10, and 24 days. As compared with sham-operated controls, PBD-operated animals had increased plasma CCK concentrations by 228, 318, and 207% at 5, 10, and 24 days, respectively. Correspondingly, pancreatic wet weight increased by 24, 61, and 87%; total pancreatic protein by 6, 57, and 73%; and total pancreatic DNA by 35, 52, and 98%, respectively. At 5 days, but not at 10 and 24 days, there was a significant increase in the pancreatic tissue DNA concentration (p less than 0.01) and [3]H-thymidine incorporation into DNA (p less than 0.02). Autoradiography showed increased [3]H-thymidine labeling index in acinar cells at 5 and 10 days after PBD (p less than 0.01 and p less than 0.005). Although not significant, ductal cell labeling index was also increased at 5 and 10 days. These findings provide evidence that, as in the rat, PBD in the hamster induces hypercholecystokininemia with ensuing pancreatic hyperplasia and hypertrophy. The hamster model may be useful for studies on the effect of endogenous CCK on pancreatic ductal cell carcinogenesis and diseases of the gallbladder, neither of which can be studied in the rat.

Animals↗
PubMed1992

Extracorporeal shock wave lithotripsy of bile duct stones: a single institution experience.

Extracorporeal shock wave lithotripsy treatment with Dornier HM3 or MPL 9000 machines was applied in 37 patients with problematic bile duct stones. General anaesthesia was not required. After one extracorporeal shock wave lithotripsy session 14/37 patients (38%) were spontaneously stone free, and additional endoscopic extraction (eight of 37) and retreatments with extracorporeal shock wave lithotripsy (seven cases) increased the stone free rate to 29/37 (78%). In three patients with intrahepatic stones, the bile ducts could not be evaluated decisively at cholangiography and ultrasonography, but they were all symptom free at 15 to 38 months follow up. If these three patients are added to the radiologically stone free patients, the overall clinical success rate was 32/37 (86%). There were no serious complications, hospital admissions, or 30 day mortality as a result of extracorporeal shock wave lithotripsy or endoscopic procedures. It is concluded that extracorporeal shock wave lithotripsy is a valuable adjunct to the non-surgical treatment of bile duct stones.

Aged↗
PubMed1992

The importance of the centroacinar region in cerulein-induced mouse pancreatic growth.

Early proliferation events within different exocrine pancreatic cell compartments were studied in mice with or without cerulein administration. A technique with 3H-thymidine labeling of DNA-synthesizing cells was used. Groups of five animals were given either cerulein (20 micrograms/kg/24 h) or saline subcutaneously twice daily. 3H-thymidine was given as a single injection, 300 microCi intraperitoneally, on the 3rd and 8th day of cerulein or saline administration, and the fraction of labeled cells in each cell compartment (3H-thymidine labeling index, TLI) was calculated on the basis of autoradiographic analyses of the tissue. In a second set of experiments the distribution of the tracer between cell compartments, when given on the 3rd day of cerulein (saline) administration, was followed over a 5-day period. The highest proliferative rate was observed in the centroacinar cell compartment (TLI, 6%), and newly formed cells were accumulated during the 5-day 'chase' period. During the same time the relative amount of labeled centroacinar cells increased and comprised 40% of all labeled exocrine cells. The relative size of the compartment did not increase, indicating increased cell turnover with loss of or possibly migration of centroacinar cells into other pancreatic cell compartments. Weak and no trophic effects of cerulein were seen on the acinar and ductal cell populations, respectively. In the control group labeled ductal cells were significantly reduced during the chase period. This was not observed in the cerulein group, indicating addition of labeled cells to this compartment from other sources. A significant non-cerulein-dependent relative increase and decrease of the ductal and acinar cell compartments, respectively, were observed during the chase period.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1992

Does vagotomy affect the growth of the pancreas in the rat?

Vagotomy has been claimed to have a growth-promoting effect on the pancreas in the rat. The present study failed to show differences in total or relative pancreatic weight and DNA content between vagally intact and vagally denervated rats, whether normoCCKemic or hyperCCKemic after pancreaticobiliary diversion during 2-8 weeks. Contrary to the claims made in several recent reports, vagotomy does not seem to influence the growth of the rat pancreas.

Animals↗
PubMed1992

Pancreatic atrophy in rats produced by the cholecystokinin-A receptor antagonist devazepide.

According to recent reports, the powerful and selective cholecystokinin (CCK)-A receptor antagonist devazepide (also referred to as L-364,718 or MK-329) is without effect on the weight of the pancreas. This has been interpreted to mean that basal and meal-stimulated endogenous CCK does not play a major role in the normal maintenance of the pancreas. In the present study we show that continuous subcutaneous infusion of devazepide effectively and dose-dependently reduced the weight of the pancreas both in normal rats and in hyperCCKemic rats (because of pancreaticobiliary diversion). The maximum reduction of the pancreatic weight was 40%. Maximum or near-maximum effects were seen with a dose of 200 micrograms/kg/h. The DNA content of the pancreas was also reduced. The reduction in weight and DNA content of the pancreas was maximal after 10 days. Provided that devazepide acts solely by inhibiting CCK-A receptors, we can conclude that endogenous CCK plays an important role in both normal and stimulated growth of the rat pancreas.

Animals↗
PubMed1992

Effect of pancreaticobiliary diversion on liver regeneration.

To ascertain whether the increase in cholecystokinin (CCK) level associated with pancreaticobiliary diversion has a cytoproliferative effect on the liver similar to that on the pancreas, we studied three groups of rats: group I (n = 19) had a pancreaticobiliary diversion and 4 weeks later a 70% liver resection, group II (n = 13) had a liver resection only, and group III (n = 6) underwent a sham liver operation. Tissue samples were taken from the liver and pancreas 48 h after the liver operation. Liver regeneration was evaluated on the basis of continuous incorporation of tritiated thymidine into hepatocytes, liver weight, and DNA content. For confirmation of the increase in CCK levels the effects on the pancreas was studied by measuring wet weight, total protein, and total DNA content. The results showed trophic effects on the pancreas, as expected, but no effects whatsoever on liver regeneration. CCK does not seem to have any role in the regulation of liver regeneration.

Animals↗
PubMed1991

Solid-cystic (papillary-cystic) tumours within and outside the pancreas in men: report of two patients.

Solid-cystic (papillary-cystic) tumours (SCT) of the pancreas are distinctive neoplasms with a predilection for young female patients. This is the first detailed report describing the occurrence of SCT in two young male patients. Except for the extapancreatic occurrence of one of the tumours (in the retroperitoneal region behind the head of the pancreas), all other clinicopathological features were identical to those characterizing the SCT in women. Immunostaining was (at least focally) positive for Lu 5 (broad spectrum keratin marker), vimentin and alpha-1-antitrypsin. The tumours were negative for neuroendocrine markers (except for neuron-specific enolase), pancreatic hormones and enzymes, pancreatic stone protein, carcinoembryonic antigen, CA 19-9 and nuclear oestrogen and progesterone receptors. This report does not support the suggested female sex hormone dependence of SCT.

Adult↗
PubMed1991

Pancreatic ductal morphology and function in primary Sjögren's syndrome.

In six patients with primary Sjögren's syndrome defined with HLA-antigens and no clinical signs of pancreatitis, the pancreatic ductal morphology (as described by endoscopic retrograde pancreatography), serum pancreatic enzymes, and fecal fat excretion were examined and secretin test was carried out. Further, oral glucose tolerance test with concomitant determination of serum insulin and C-peptide were done. All patients had at least two pathological signs of exocrine function and/or ductal morphology. There was no evidence of endocrine malfunction. The findings are suggestive of the existence of a clinically silent pancreatitis, perhaps of autoimmune etiology.

Aged↗
PubMed1991

Diagnosis of pancreatic cancer. Alteration of glucose metabolism.

Alterations of glucose metabolism occur frequently in association with pancreatic cancer. Whether diabetes predisposes or is a consequence of pancreatic cancer has not been settled. We have found that induced diabetes prevents development of pancreatic cancer in the hamster model and that genetically diabetic Chinese hamsters resist pancreatic cancer induction, whereas the nondiabetic strain does not. Also, preliminary clinical data indicates that diabetes is associated with pancreatic cancer and that the two conditions develop simultaneously. We have found diabetes or impaired glucose tolerance in 76% of the pancreatic cancer patients at the time of cancer diagnosis. The diabetic condition improved after 75% pancreatectomy. Although the preliminary observations suggest that diabetes accompanies the disorder of pancreatic cancer, additional data are needed before any conclusion can be drawn.

Animals↗
PubMed1991

Selective deficiency of pancreatic amylase.

Two patients with specific pancreatic amylase deficiency are described. The greatly reduced pancreatic amylase activity was not due to an enzymatically inactive amylase molecule but to an almost complete absence of the molecule itself. The findings are of diagnostic importance as they show that low pancreatic amylase activity in serum or duodenal aspirates, or both, does not necessarily represent chronic exocrine pancreatic disease such as chronic pancreatitis, carcinoma of the pancreas, or cystic fibrosis but may be an isolated finding. In one of our patients a familial occurrence was shown, indicating a congenital deficiency.

Adult↗
PubMed1991

Proliferative response of different exocrine pancreatic cells after surgical pancreaticobiliary diversion in the rat.

Pancreaticobiliary diversion (PBD) is known to induce chronic, endogenous hypercholecystokininemia causing pancreatic growth in rats. In the present study the proliferative response of the different exocrine pancreatic cells was studied by administration of 3H-thymidine, 1 mCi/kg, given 1 h before the rats were killed and 5, 10, 20, and 40 days after PBD. DNA and 3H-thymidine uptake, both expressed per 1 mg of pancreatic tissue, were significantly increased on day 5. The nuclear labeling index was increased fivefold in both the acinar and ductal cell group. In the centroacinar cell group the labeling index was increased on day 10. In conclusion, we found that the proliferative activity after PBD occurred during the first 10 days and that the ductal cells were forced into proliferation to the same extent as the acinar ones. These findings are of interest for future studies of hormonal influences on the development of pancreatic carcinoma.

Animals↗
PubMed1991

Availability of energy substrates during liver regeneration in malnourished rats.

A retarded liver regeneration rate during malnutrition has previously been reported. To explore the mechanisms, some energy substrates were studied during liver regeneration and malnutrition. Forty-one rats were allocated to one of three groups (G): GI were normally nourished rats, which were partially hepatectomized; GII were semistarved rats, which were partially hepatectomized; and GIII were normally nourished rats, which were sham-operated. Biopsy specimens were taken from the liver at the time of partial hepatectomy and when they were killed after 48 h of regeneration. The samples were analysed for adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), glycogen, lactate, and pyruvate, and energy charge potential (ECP) was calculated. ATP, ECP, and glycogen levels were decreased in the malnourished group before the resection. After 48 h of regeneration all values were unchanged in the normally nourished group, whereas ATP, ADP, and ECP were increased in the malnourished group compared with the initial values. The lactate/pyruvate quotient was increased in malnourished rats compared with normal rats. The results suggest that the energy supply is not a limiting factor for the reduced regeneration rate seen in malnourished animals.

Adenosine Diphosphate↗