Biomedical subjects

I Ikarashi

Publications and source records attributed to I Ikarashi.

4 recordsLinked to original sources

PubMed1994

[Comparison of urine volume, glomerular filtration rate, excretion and fraction of reabsorption of major substances in urine in three different rat strains].

Urine volume (UV), Glomerular filtration rate (GFR) and Fraction of reabsorption (FR) of water, Ca, IP, Na+, K+, Cl-, Glu and UN, which are significant in kidney function analysis, were investigated and compared in three rat strains (WI, SD and F344). UV/body weight ratios were higher in SD rats as compared to other strains. The GFR difference was not significant, but the FRH2O difference was significant in three rat strains. FR of Ca, K+ and Glu showed no significant differences in three different rat strains. SD and F344 were significantly lower than WI in FR of IP. FR of Na+, Cl- and UN were significantly different in three rat strains without Cl- between SD and F344, and UN between WI and SD.

Animals↗

PubMed1993

Effect of neonatal gonadectomy on lymphocytes population and blast formation in aged rats.

In male rats gonadectomized at birth, the blast formation reaction of lymphocytes was significantly lower at 114 weeks of age, than that of non-gonadectomized controls, whereas the lymphocyte subpopulation was similar. The thymus of neonatally castrated rats of age 114 weeks was histologically normal, decreasing in weight of 68% of that of non-castrated rats.

Aging↗

PubMed1993

A new visual method for evaluating multiple data (Diagnostic Radar Chart) in general toxicological study.

A Diagnostic Radar Chart method was devised for use in the general assessment of the toxicity of drugs on the basis of visual patterns in toxicity studies. Its usefulness was evaluated on the findings of 32 laboratory items in 3 groups of animal models with experimental pathological conditions. The chart was found to facilitate intuitive discrimination among three types of pathological animal models, evaluation of sexual differences and evaluation of individual differences among the animals for each item. Therefore, if patterns are constructed on the Diagnostic Radar Chart for each of the drugs usable for preparing animal models with typical experimental pathological conditions, the chart is thought to be useful for evaluation or identification of the toxicity of an unknown drug.

Anemia, Hemolytic↗

PubMed1990

[Blastogenic response in the rat lymphocyte using glucose consumption test].

To estimate the immune function of the rat, the blastogenic response of rat lymphocytes in blood drawn from the vena cava was investigated. The recovery rate of lymphocytes from venous blood was 33.2 +/- 2. 23% and their viability rate was 99.8 +/- 0.25%. Three kinds of mitogens (phytohemagglutinin-M, concanavalin A and pokeweed mitogen) at three different concentrations (high, middle and low) were added to lymphocyte suspensions, and the suspensions were cultured under 5% CO2 incubation. Cultured suspensions were served for glucose consumption test and values were calculated (glucose consumption test-stimulation ratio (GCT-SR)). Incidence of blast cells was also observed by morphology. In both the GCT-SR and blast cell incidence, the highest values, 41.2% and 50.8%, respectively, were obtained after stimulation with phytohemagglutinin-M at the concentration of 0.1 ml/ml. The results were statistically analyzed by the variance of means. On both the GCT-SR and blast cell incidence, values obtained in each condition (kind of mitogens on the concentration used) were all statistically significant (all P less than 0.01). The correlation coefficient between the GCT-SR values and the blast cell incidence was 0.97 (P less than 0.01). From these results, we concluded that the glucose consumption test using rat blood lymphocytes can be used as a safe and simple method to measure the immune function of rats.

Analysis of Variance↗