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I Ionova

Publications and source records attributed to I Ionova.

At least 19 recordsLinked to original sources

[Hygienic research on meat-dressing shops and enterprises].

Observations were made on the hygiene state of the meat-conditioning departments and enterprises of 13 meat-dressing combines. Both qualitative and quantitative investigations were carried out with regard to 9 bacteriologic indexes. The result of studies on a total of 1,042 samples of washings taken from equipment, working tools, machines, working milieu, and hands and aprons of workers prior to beginning work showed that in case all regulations of cleaning, washing, and disinfection were observed the residual microflora, and, more specifically organisms that were of interest from a sanitary point of view were lowered to minimum counts. No pathogenic Staphylococcus and Salmonella organisms were isolated. It was found that during work the count of indicative organisms considerably rose. No pathogenic staphylococci were isolated. In one case only were there Salmonella typhimurium organisms on the surface of a circular saw for carcass cutting. The overall bacteriologic investigation of a total of 137 samples of minced meat and meat balls and other products prepared with such meat revealed that the average counts of various organisms ranged as follows: aerobic mesophiles--3,653 830/g, and coliforms--31,748/g; Escherichia coli organisms were demonstrated in 10.22 per cent of the cases, B. proteus--in 1.46 per cent, and pathogenic staphylococci--in 3.65 per cent of the samples. None of the samples, weighing 25 g each showed the presence of Salmonellae. No correlative dependence was found between the coli titer and the coli index of the products.

Animals↗

[Electrophoretic method of demonstrating antibiotic residues in meat and eggs].

Studies were carried out on Smither and Vanghan's electrophoretic method with the use of high voltage. The method was found to be readily reproducible and to have good sensitivity. It could be successfully used in the demonstration and identification of residual amounts of antibiotics in meat and eggs. It was also established that with the use of agarose of pH 6.0 amino glycosides, beta-lactams, flavomycin, spectinomycin, and monensin showed higher migration capacity. The same value of pH was contributive to increasing the migration distances with macrolites, zincbacitracin, and tetracyclines. It was demonstrated that the use of electric current at 50 MA made it more favourable to separate and identify antibiotics. B. subtilis BGA was found to be more sensitive than B. mycoides v. cereus ATCC 11778 with regard to the antibiotics used and it could be made use of in the demonstration of minimal amounts.

Animals↗

[Chemical composition of the meat from chickens treated with aflatoxin B1].

Studies were carried out to establish the effect of aflatoxin B1 on some qualitative indices of poultry meat. It was found that the use of aflatoxin B1 led to a decrease in the live weight as well as to changes in the chemical composition of meat-increase in the water content, decrease in the amount of fats and proteins, and drop of its biologic value. Most clearly manifested were the changes when aflatoxin B1 was applied at the rate of 37.5 micrograms daily, in the course of 30 days. It was also found that withdrawal periods of 7 days did not contribute to the elimination of the changes in the chemical composition of poultry meat.

Aflatoxin B1↗

[Chemical composition of the meat of chickens treated with antibiotics].

Studies were carried out on the chemical composition of meat obtained from a total of 150 Leghorn birds at the age of four weeks, treated with nine antibiotic preparations. When the antibiotics were applied at therapeutic rates certain changes took place in the amount of total nitrogen as well as in the amino acids tryptophane and hydroxi-proline, which depended on the type of the antibiotic used. The study of muscle samples from the test groups revealed negligible rise (P greater than 0.05) of the fats and water to the detriment of total nitrogen as against the values of the same indices of the controls. Demonstrated was the higher biologic value of white meat proteins as shown by the control group as compared with the test groups. The withdrawal time was found to be six days during which full elimination of the antibiotic preparations from the body of the birds was achieved, and there was no rise both of the total nitrogen and of the index of the tryptophane/hydroxi-proline ratio.

Animal Feed↗

[Trial of kormogrisein-40 as a growth stimulant in pigs].

An experiment was carried out with 36 pigs, weighing 8 to 70 kg, divided into 4 groups to test the growth-promoting effect of kormogrisin-40 given with the feed. The animals were fed on one and the same standard combined feed--a basic ration to which antibiotics were added in the form of a premix as follows: group I (controls); group II--zinc-bacitracin in conc. of 40 ppm; group III--grisin in conc. of 5 ppm; and group IV--grisin in conc. of 12 ppm. It was ascertained that growth stimulation was best manifested with group IV--the average daily gain increased by 17 per cent, and the consumption of feed per kilogram of gain decreased by 13.2 per cent. Second in effect ranked group III--13.0 and 9.6 per cent respectively. When concentrations of 5 and 12 ppm of grisin were used with pigs, and these were slaughtered without observing any withdrawal times there were residual amounts of the antibiotic in the meat and the parenchymal organs.

Animals↗

[Fatty acid content of the lipid fraction of the meat of chickens fed mixed feeds with added bacterial protein].

Studies were carried out to establish the content of fatty acids in the lipid fraction of the meat of birds that were given combined feeds with the supplement of bacterial protein. A Perkin Elmer gas chromatograph with a flame-ionization detector were used to evaluate the fatty acid content. It was found that with the increase in the bacterial protein content of the feed mixtures there was a rising trend in the sum total of the unsaturated fatty acids (C16:1, C18:1, C18:2) and a lowering one of the saturated fatty acids (C14, C16, C18) as against their content in the meat of the control group.

Animal Feed↗

[Quality of meat from lambs fattened on feed mixtures with added bacterial protein].

Physico-chemical, microbiologic, and histologic investigations were carried out with meat and parenchymal organs of lambs offered feed mixtures with a supplement of bacterial protein on methanol basis in fattening the animals up to 35 kilograms. The addition of 5 per cent bacterial protein which replaced in terms of protein equivalent 60 per cent of the sunflower oil meal and totally the amount of urea in the mixtures had no negative effect on the physical and chemical composition of the meat and its biologic value. Histologically, no degenerative changes in the skeletal muscles and parenchymal organs were found. There was hypofunction of the thyroid gland in the test lambs. No pathogenic and occasionally pathogenic organisms were found in the sampled meat and organs.

Animal Feed↗

[Chemical and microbiological studies of the meat of broilers fed mixed feeds with a bacterial protein supplement].

Physico-chemical and microbiologic studies were carried out with meat of broilers that had been offered combined feed mixtures in which part of the soybean meal protein was replaced by microbial one through the incorporation of 4, 8, and 12 per cent industrial single cell protein. The bacterial protein was shown to have no adverse effect on the physico-chemical composition of poultry meat. Demonstrated was a trend toward the separation in individual layers of more fats in the carcass with the increase in the percent of biomass of the combined feed mixtures for poultry. Considering the content of total nitrogen, fats, and ashes in the poultry meat most expedient was the incorporation of 4 percent single-cell protein in the feed mixtures for broilers. No pathogenic and conditionally pathogenic organisms were found in the meat of broilers.

Animal Feed↗

[Presence of salmonellae and coliform bacteria in ground meat and the sources of its contamination].

Studied were a total of 14,944 samples of minced meat for the presence of Salmonellae and 608 samples for establishing the coli-titer. Examined were also bacteriologically 5651 washing samples from the workers' hands, the machines, equipment and stock implements in the shops for the production of minced meat prior to starting work as well as 3721 washing samples from the same for the presence of coliforms. It was found that 1.81 per cent of 14,188 samples of minced meat were contaminated with as many as 17 serotypes of Salmonella organisms belonging to the B, C and E serogroups. It was believed that the primary source of Salmonellae in minced meat were the clinically normal Salmonella carrier animals apart from the occasional contamination during the individual technologic stages of processing. The smallest amount of minced meat in which coli bacteria (titer) could be found varied within the range of 0.01 to 0.0001, 53 out of the investigated 608 samples, i. e. 8.72 per cent showing a titer of 0.01, 477 samples (78.45 per cent) having a titer of 0.001, and 78 samples (12.88 per cent) having a titer of 0.0001. Of the examined samples washings 25.69 were positive for coli bacteria, and 0.27 per cent--for Salmonella bacteria, which spoke of improper disinfection.

Animals↗

[Enterococcal studies of uncut raw dried meat products].

Studies were carried out on a total of 75 samples of raw-dried meat products of a compact type--dried beef, Gabrovska Keiser dried meat, pig salted neck, rolled meat Trapezitsa, V. Turnovo delicacy, and Elena fillet, to establish the amount of Staphylococcus bacteria. It was found that the enterococcus titer of this type of meat products varied from 10(-1) to 10(-4), depending on the product. Most strongly contaminated with enterococci proved the pig salted neck. The higher amounts of enterococci in the studied products as well as their higher resistance to the action of unfavourable factors (ph, NaCl, water content) showed their advantage over the coliform bacteria as sanitary-index organisms in raw-dried meat products of a compact type. Various ratios of all species of enterococci were established in these products. Str. faecius was most often found (32.44 per cent), followed by Str. faecalis (26.22 per cent), Str. faecalis var. liquefaciens (16.00 per cent), and Str. durans (12.00 per cent). Str. faecalis var. zymogenes (8.00 per cent) and Str. bovis (5.33 per cent) were isolated less frequently from some of the products only.

Animals↗

[Agents for the aerosol disinfection of the air in meat freezers].

Studies were carried out on the use of aerosol disinfection with lactic acid and sorbic acid at rates of 15, 30, and 50 mg per 1 m3 air in chambers for the conservation of meat and meat products at temperatures within the range of 0 to 2 degrees C. Moulds of the Penicillium genus and psychrophile organisms (Pseudomonas, Alkaligenes, and Flavobacter) were used as test microbial species. It was found that the disinfection effect of lactic acid used in the form of aerosol was the same as that of sorbic acid when psychorophiles served as test bacteria, and was essentially better in the disinfection of the air in refrigerators and the surface of meat when Penicillium moulds were used. In concentrations of up to 50 mg/m3 both lactic acid and sorbic acid had no adverse effect on the organoleptic indices of meat during refrigerator storage.

Aerosols↗

[Physical methods (ultrasound) and disinfecting agents for decontaminating the water in poultry meat processing].

Productional and laboratory experiments were carried out to study the effect of ultra sound with a view to decontaminating the water in the vats for cooling poultry carcasses and skin cover as well as that of disinfection means (inkosan, actofor, yomil S, chalamide, and chloramin). In vitro studies revealed that the use of ultra sound in the decontamination of cooling water and poultry meat surface did not produce the necessary effect. The iodofor preparations inkosan and yomil S as well as the agents chalamide and chloramine added in fixed concentrations to the water in cooling vats were not shown to be able to inactivate Salmonella organisms on the surface of experimentally contaminated poultry carcasses. Inkosan at the rate of 1:1000, and yomil S at 0.2 and 0.4 per cent produced a 100 per cent bactericidal effect against the microflora in vat waters regardless of their initial contamination. Except for yomil S at 0.4 per cent these agents did not affect the organoleptic properties of poultry meat.

Animals↗

[Microbiological study in pork production].

Investigations were carried out in production conditions on the sources of surface contamination of pork with regard to sanitary-indicative and pathogenic (Salmonellae) organisms. A total of 74 series of experiments were carried out, every series making use of the following: 10 fecal samples taken on the preslaughter premise; the results of studying the skin cover of 3 animals prior to washing, after washing, and after stunning, employing the method of Dryabina in studying skin areas of 25 cm2; as many as three washings taken from within the path from the place where the animals were washed to the place where they were stunned; the results of studying the surface of the meat produced (from 3 animals in each case) and at the site of starting skin removal, with sampling the abdomen muscles and the group along with sampling the meat itself after cleaning and washing it - taking impression preparations, resp., employing the contact method with a 44 cm2 surface area of the agar. No Salmonella bacteria were found in the 1628 analyses and fecal samples of the skin of pigs intended for slaughter. The washing of the animals lowered more than 70 times the total bacterial contamination of the skin cover. It was found that the site of starting skin removal and the muscles of the abdomen and those of the forelimbs were most strongly contaminated.

Animals↗

[Carrier state and body distribution of Salmonella bacteria in healthy piglets and calves].

Studies were carried out to establish the carrier status and the topographic distribution of Salmonella bacteria in the carcass and organs of healthy pigs and calves. A total of 3980 samples were subjected to investigation (m. gracilis, lymphonodus subilicus, lymphonodus praescapularis, lymphonodi mesenterici, liver bile duct, gallbladder, lymphonodi hepatici, and spleen and samples from caecum content taken from 560 slaughtered normal pigs), and a total of 2400 samples (m. gracilis, caput longum of m. triceps brachii, lymphonodus praescapularis, lymphonodus subilicus lymphonodi hepatic, lymphonodi mesenterici, gallbladder, spleen, heart, and kidney and samples from the caecum content taken from 200 slaughtered normal calves. The investigations were carried out with methods routinely employed in the microbiological science and practice with special reference to Salmonella bacteria. It was found that 4.29 per cent of the slaughtered healthy pigs were carriers of Salmonella organisms, 10 serotypes of the groups B, C1, C2, D and E1 of Salmonellae being isolated. Most frequently isolated serotypes in a descending order were: S. anatus, 7 strains; S. cholerae suis, 6 strains; S. derby, 5 strains; S. agona, 4 strains; S thompson and S. dublin, 2 strains; and S. essen, S. lille, S. kottbus, and S. enteritidis, 1 strain. The highest percent of Salmonellae in the slaughtered normal pigs was found in the caecum content (3,75%). No Salmonellae were found in the samples from the slaughtered 200 normal calves and the muscle and carcass lymph node a samples taken from the investigated 560 pigs.

Animals↗

[Chemical composition of the meat and liver of pigs fattened with mixtures containing a bacterial protein supplement].

The chemical composition of the meat and liver of pigs fattened under production conditions was established with the addition of 3, 4, and 5 per cent bacterial protein to three experimental groups of animals, respectively. A control group of pigs was also used fed with no biomass in their feed. The trial lasted 104 days, the liveweight of the animals in the beginning of the experiment being about 35-40 kg, and at the end of it--100--115 kg. It was found that by the contents of water, total, extractable and protein nitrogen, fats, ash, essential and common proteins, tryptophane, and hydroxyproline and the index of their ratio, the pH value and calories as well as by organoleptic evaluation the samples of the investigated m. longissimus dorsi and liver of the test animals did not differ essentially (P greater than 0.05) from the respective values of the controls.

Animal Feed↗

[Staphylococci in uncut raw dried meat products].

Studied were a total of 75 samples of raw-dried meat products in a compact form (dried beef, Gabrovska Keiser dried meat, pig salted neck, Trapezitsa rolled meat, Veliko Turnovo delicacy, and Elena fillet) produced at 11 Meat Works for the presence of staphylococci. It was found that the Staphylococcus titre in such raw-dried meat products was within the range of 10(-3) to 10(-7). Veliko Turnovo, Gabrovska, and Trapezitsa products were shown to be most contaminated. Coagulase-positive staphylococci were demonstrated in as much as 0.1 g of them--in the case of Elena fillet all samples were positive, with dried beef--10 samples, with Trapezitsa--7 samples, with Gabrovska--4 samples, and with pig salted neck 2 samples. The isolated strains of coagulase-positive staphylococci (48) were chiefly of bovine origin, and were typed by means of a mixed phage set comprising phages of groups II, III, and IV. The most commonly established phage types were 53/6/42D77 and 42D/102/107/117/119/77/42E. The compact raw-dried meat products were shown to contain all species of staphylococci. Staphylococcus epidermidis was most often isolated (with the exception of Elena fillet).

Animals↗

[Morphological and enzymatic changes in chicks treated with antibiotics].

The live, kidney and skeletal muscules of 54 chickens fed experimentally with bacitrazin, gallimycin, oleandomycin, tetracyclin, tylan, chlornitromycin, streptomycin and penicillin, and of 10 control birds were investigated pathomorphologically and histochemically (succinate--and lactate-dehdrogenase and esterase). Parenchymal dystrophy in the epithelial cells of the liver and kidney and areactive necrotic foci amid the parenchyma were assessed. Dystrophic and necrotic changes observed in the muscle fibres of treated birds. Reduced enzyme activity was proven. The studies indicate that the morphological and enzyme changes in the liver, kidney and skeletal muscules of chickens treated with bacitrazin, gallimycin, chlornitromycin and streptomycin are greater than these observed in birds fed with tylan, tetracyclin, olenadomycin and penicillin. It is proven that the morphological and enzyme changes established in healthy chickens fed with prophylactic antibiotic doses are due to the antibiotics applied.

Animals↗