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I J Check

Publications and source records attributed to I J Check.

49 records · Page 3Linked to original sources
PubMed1982

Diclonal gammopathy in chronic cold agglutinin disease.

Serum from a patient with chronic cold agglutinin disease (CCAD) contained two paraproteins identified as IgM kappa and IgM lambda by immunoelectrophoresis and confirmed by immunofixation on high resolution agarose gel electrophoresis. The IgM kappa band was completely removed by incubation of the serum at 4 degrees with ficin-treated O Rh-positive red blood cells, and partially removed by incubation with cord cells, suggesting that it reacted with I antigen. The IgM lambda band was unaffected by these absorptions. Previous investigators have shown CCAD to be a monoclonal gammopathy, usually IgM kappa. The presence of both IgM kappa and IgM lambda paraproteins in this patient with the IgM kappa showing cold agglutinin activity is unique. The patient has a lymphoplasmacytic infiltrate in the bone marrow. These data suggest that CCAD may be part of a spectrum of disorders in which paraproteins are produced and are stimulated by factors unrelated to the target red blood cell antigens.

Aged↗
PubMed1982

Stimulation of NK-like CMC by skin test antigens in vitro is depressed in patients with SLE.

Peripheral blood mononuclear cells isolated from patients with systemic lupus erythematosus (SLE) developed lower levels of skin test antigen stimulated natural killer cell like cell-mediated cytolysis (NK-like CMC) than did similar cells from age and sex matched non-SLE volunteers. When the subjects were grouped according to disease activity the cells from the patients with mild disease activity developed lower levels of NK-like CMC than did those from the non-SLE control subjects. The cells isolated from SLE patients who had moderate to severely active disease developed little if any NK-like CMC after exposure to various skin test antigens. Greatly decreased skin test antigen stimulation of NK-like CMC in the cells from the SLE patients with more active disease did not appear to be due to therapy with corticosteroids or to a suppressor effect on this response by monocytes present in the peripheral blood mononuclear cell populations.

Adult↗
PubMed1981

Prognostic significance of immunological tests in lung cancer.

We performed a battery of tests on peripheral blood samples from 94 patients with lung cancer to determine the extent to which immunological depression was due to abnormal lymphocyte function, as compared to changes in the number of lymphoid cells in the peripheral blood or in the efficiency of purification of cells in Ficoll-Hypaque gradients in preparation for testing. The percentage of lymphocytes in the gradient-derived cell suspension (%LG) was the most informative test. It decreased significantly with advancing stage of cancer and could predict survival of patients with uniform stage. The %LG correlated with survival better than any other test when multivariate analyses of all test combinations were performed. Low values of %LG reflected both the depressed lymphocyte counts and the altered buoyant density of the leucocytes of many patients with advanced cancer. A large proportion of the depression in other immune function tests was statistically attributed to changes in %LG. We concluded that this simple measurement provides valuable information about patients with lung cancer.

Carcinoma, Bronchogenic↗
PubMed1980

Studies on stimulation of cell-mediated cytotoxicity by skin test antigens. I. Candida antigen stimulation of cell-mediated cytotoxicity in vitro correlated with the skin test response to candida antigen in vivo.

The magnitude of the skin test response in a group of normal volunteers to intradermal Candida antigen correlated closely with the level of cytolytic activity stimulated by Candida antigen in vitro in the peripheral blood lymphocytes from those same individuals. The cytolytic activity stimulated by Candida antigen was cell mediated, and cold target inhibition studies demonstrated that Candida antigen-stimulated effector cells were capable of cross-species (i.e., nonspecific) killing. Analysis of the Candida antigen-induced effector cell population for various cell markers did not enable absolute identification of the cell responsible for the killing. The findings in this study indicate that the stimulation of cell-mediated cytolysis by Candida antigen may be related to the delayed-type hypersensitivity response produced by the same antigen.

Animals↗
PubMed1980

The relationship between cytotoxicity and prognostically significant histologic changes in lymph nodes from patients with cancer of the breast.

Axillary lymph nodes obtained from 37 patients with cancer of the breast were used in a microcytotoxicity assay against a human mammary cancer cell line, ALAB. Cell suspensions made from 60 individual lymph nodes and 51 lymph node pools were tested. Each lymph node was graded for the extent of the histologic changes of sinus histiocytosis (SH), paracortical hyperplasia (PCH), and germinal center hyperplasia (GCH). High levels of cytotoxicity correlated significantly with the presence of SH and PCH. When multiple lymph nodes of individual patients were pooled and tested, a high intensity of GCH correlated with a low degree of cytototoxicity. The cytotoxic activity of pooled lymph nodes from different axillary regions was studied in 20 patients. In 7 of these patients, low axillary lymph node cells were more cytotoxic than high axillary node cells. The reverse was found for 7 patients, and there was no difference in cytotoxicity between axillary regions in 6. Cytotoxic lymph node pools had high SH and low GCH whether they were obtained from the low or high region. Noncytotoxic lymph node pools from the high axillary region had a low intensity of all three histologic reactions. In contrast, noncytotoxic lymph node pools from the low axillary regions had high intensities of SH, PCH, and GCH. These results suggest that germinal center hyperplasia in the lymph nodes nearest an advancing tumor is associated with a local suppression of cytotoxic cell activity.

Adult↗
PubMed1980

Prediction of survival in gynecological cancer based on immunological tests.

Patients with advanced cancer have a depressed immunological function. We performed a battery of tests on peripheral blood samples from 42 patients with gynecological cancer to determine the extent to which this depression was due to abnormal lymphocyte function, as compared to changes in the number of lymphoid cells in the peripheral blood or in the efficiency of purification of cells in Ficoll:Hypaque gradients in preparation for testing. The percentage of lymphocytes in the gradient-derived cell suspension (% LG) and the absolute lymphocyte count were more informative than mitogen stimulation, mixed leukocyte culture, and T- and B-cell measurements. Both values decreased significantly with the advancing stage of cancer, and we were able to predict survival of patients with uniform stage of disease. The % LG correlated with survival better than did any other test when multivariate analyses of all test combinations were performed. Low values of % LG reflected both the depressed lymphocyte counts and the altered buoyant density of the leukocytes of many patients with advanced cancer. A large portion of the depression in other immune function tests was statistically attributed to changes in % LG and the lymphocyte counts. We concluded that these two simple measurements provide valuable information about patients with gynecological cancer.

Female↗
PubMed1980

Prediction of survival in head and neck cancer based on leukocyte sedimentation in Ficoll-Hypaque gradients.

A battery of immunologic tests were performed on 42 patients with recurrent cancer of the head and neck whose median survival time was 6.8 months. Two tests correlated with survival: total white blood cell (WBC) count and the percentage of lymphoid cells (%LG) in the Ficoll-Hypaque gradient separated leukocyte fraction of blood. Patients with abnormally low values of %LB had a poor prognosis, as did patients who had greater than 8000 WBC count. Of the variation in %LG, 43% was due to the differences in differential WBC counts among the patients, with the remaining variation attributed to changes in the buoyant densities of the leukocytes. These data suggest that further study of the factors effecting buoyant density of cells may be clinically useful.

Cell Count↗
PubMed1979

Lymphocyte stimulation by yeast phase Histoplasma capsulatum in presumed ocular histoplasmosis syndrome.

We measured skin reactions serum antibody, and lymphocyte stimulation to Histoplasma antigens in a series of patients with presumed ocular histoplasmosis syndrome and in controls. The most sensitive test, lymphocyte stimulation to H. capsulatum sonicate, also correlated with severity of the disease. Lymphocyte stimulation to Histoplasma may be a useful adjunct to the monitoring of presumed ocular histoplasmosis syndrome.

Antibodies, Fungal↗
PubMedDate not supplied

IgG subclass distribution in patients with multiple myeloma or with monoclonal gammopathy of undetermined significance.

Multiple myeloma provides a unique model for studying factors affecting IgG isotype distribution in humans. Evaluations were made as to whether monoclonal immunoglobulins (M-proteins) of different IgG isotypes are associated with different extents of hypogammaglobulinemia and whether all residual subclasses are decreased comparably. The isotype patterns were analyzed in the context of the gene order of the constant regions of gamma (gamma) heavy chains on chromosome 14. Using monoclonal antibody-based immunoenzymometric assays, IgG subclasses were quantitated in the sera of 50 patients having IgG M-proteins, 38 with multiple myeloma and 12 with monoclonal gammopathy of undetermined significance. Thirty-three (66 percent) patients had IgG1, nine (18 percent) had IgG2, four (8 percent) had IgG3, and four had IgG4 M-proteins, paralleling the normal IgG subclass distribution. The concentration of residual IgG (sum of the evaluatable polyclonal IgG subclasses) was significantly decreased in patient sera (p less than 0.05). However, in only seven (14 percent) of the patients were all three subclasses below the reference range, suggesting some selectivity of immunosuppression. Patients with M-proteins of different IgG subclasses had markedly different patterns of suppression. Patients with IgG2 M-proteins (78 percent) were more likely to have depressed residual IgG than patients with IgG3 (50 percent), IgG1 (27 percent) or IgG4 (0 percent) M-proteins. Some patients had deficits of only one or two IgG subclasses. When considering all sera together, residual IgG1 was disproportionately reduced, followed by residual IgG2, IgG3, and IgG4. Next it was determined whether or not patterns of suppression were predicted by the gamma heavy-chain gene order (5' to 3'): gamma 3, gamma 1, gamma 2, gamma 4, as seen in some other immunologic disorders. Interestingly, the normal isotypes encoded by genes in juxtaposition to that of the M-protein were most often decreased (p less than 0.05). Thus, the patterns of hypogammaglobulinemia in multiple myeloma are heterogeneous. They may be influenced by the M-protein itself, possibly through interactions with regulatory cells. In addition, factors at the gene rearrangement level may contribute.

Aged↗