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Biomedical subjects

I Jönsson

Publications and source records attributed to I Jönsson.

11 recordsLinked to original sources
PubMed1997

Human immunoglobulin isotype and IgG subclass response to different antigens of Moraxella catarrhalis.

Enzyme immunoassays were tested for the determination of antibodies of different isotypes and IgG subclasses to Moraxella catarrhalis in human sera. An assay based on an outer membrane protein preparation (OMP) as antigen was compared to assays using whole bacterial cells and a purified lipopolysaccharide preparation. There was a good correlation between the results obtained with the OMP preparation and the whole-cell antigen. In paired sera, optimal sensitivity was obtained by using the OMP preparation as coating antigen and testing for a rise in IgG3 antibodies. However, patients with high levels of antibodies in acute serum had no or only an insignificant antibody response during infection.

Adult↗
PubMed1997

A case of Plesiomonas shigelloides cellulitis and bacteraemia from northern Europe.

Bacteremia caused by Plesiomonas shigelloides is a rare event, often associated with consumption of seafood and fresh or estuarine water in temperate or tropical climates. Most patients have showed underlying health disorders. Here we present a case of P. shigelloides septicaemia and cellulitis of the left hand associated with fish handling in Northern Sweden (65 degrees latitude north). The patient, who suffered from multiple myeloma, recovered uneventfully after initial treatment with intravenous cefuroxime followed by a course of oral ciprofloxacin. P. shigelloides seems to be ubiquitous in freshwater world-wide and may cause invasive infections also in cold climate areas.

Aged↗
PubMed1995

Lack of serotype-specific antibody response to lipopolysaccharide antigens of Moraxella catarrhalis during lower respiratory tract infection.

An enzyme immunoassay (EIA) was used to determine the antibody response to different serotypes of lipopolysaccharide (LPS) antigens of Moraxella catarrhalis in adult patients with lower respiratory tract infections (LRTI). Moraxella catarrhalis was isolated from sputum or nasopharyngeal samples from 20 patients with LRTI. Sixteen of the isolates were serotype A, four were type B and none were type C. The antibody response to the different LPS serotypes was determined in paired sera from patients suffering from LRTI. In addition to the 20 patients with Moraxella catarrhalis isolated (Group 1), a group of seven patients with LRTI of unknown etiology (Group 2) and a group of ten patients with LRTI of known other bacterial etiology (Group 3) were selected for this study. An increase in antibody levels of > 1.5-fold (convalescent-/acute-phase serum) was recorded in approximately half of the patients, not only in the first group (Moraxella catarrhalis isolated) but also in the other two groups. However, in the first and second groups there was a correlation between an increase in antibody levels in the LPS EIA and in an EIA using whole bacterial cells as antigen. In the group of patients in whom Moraxella catarrhalis was isolated, the antibody response to LPS antigens was not serotype specific. The antibody response to type-A and type-B LPS was more predominant than the response to type-C LPS in most patients.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1994

Significance of isolation of Moraxella catarrhalis in routine cultures from the respiratory tract in adults: antibody response studied in a whole cell EIA.

The significance of the isolation of Moraxella catarrhalis from sputum or nasopharynx was studied in patients treated at an infectious disease clinic. A whole-cell enzyme immunoassay was used to detect a specific antibody response to M. catarrhalis during infection. In all, 27 patients with respiratory tract infections and 4 with other infections were studied. Titre rises were recorded in 11/23 patients with lower respiratory tract infections, whereas patients with common cold or infections elsewhere all had negative serology. In patients with acute bronchitis, 7/10 patients responded with a significant titre rise. Patients with a low titre in their acute serum sample were those who responded with a titre increase during infection. The findings indicate that isolation of M. catarrhalis from sputa and nasopharyngeal samples in adults is of value for the etiological diagnosis of acute bronchitis and other lower respiratory tract infections, and is therefore important for the choice of drug for treatment, as many isolates are resistant to beta-lactam antibiotics.

Adolescent↗
PubMed1993

Serological cross-reactions between Moraxella (Branhamella) catarrhalis and other oropharyngeal bacteria.

Oropharyngeal bacteria belonging to different species were tested for serological cross-reactions with Moraxella catarrhalis using sera from immunized rabbits. Sera were tested using immunofluorescence, an enzyme immunoassay (EIA) and immunoblotting. On immunofluorescence, significant cross-reactions were demonstrated with beta-hemolytic streptococci group A and group G, as well as with streptococci of the viridans group. Some cross-reactions were also noted with Neisseria meningitidis. In the EIA, strong cross-reactions were demonstrated with beta-hemolytic streptococci. No cross-reactions were obtained with Streptococcus pneumoniae, Haemophilus influenzae or common oral Neisseria. The results are of importance for the interpretation of serological tests to detect infections with Moraxella catarrhalis, and for the development of methods for detection of antigens in samples from the respiratory tract.

Animals↗
PubMed1992

Variability of surface-exposed antigens of different strains of Moraxella catarrhalis.

For serological diagnosis of infection with Moraxella (Branhamella) catarrhalis it is important to determine if there is variability of antigenic properties among different strains. Cross-reactions of nine strains were investigated by an immunofluorescence test using sera from immunized rabbits. All titres but one were 1:256 or higher, the highest being 1:4096. Thus a high degree of antigenic similarity was demonstrated among different strains of Moraxella catarrhalis. However, the homologous titres of six sera were 2 to 16 times higher than the titres for other strains indicating strain variations in antigenic properties of some surface components. There was no correlation between lipopolysaccharide type and titre in the immunofluorescence test.

Animals↗
PubMed1990

Rapid aetiological diagnosis of pneumonia based on routine laboratory features.

The values of some basic laboratory features on admission to hospital were recorded and compared in 418 adult patients with community-acquired pneumonia, namely erythrocyte sedimentation rate, C-reactive protein, white blood cell (WBC) count, serum lactate dehydrogenase (S-LD), serum alanine-aminotransferase, and serum sodium. Discriminant analysis was performed to obtain an aetiological diagnosis. WBC value of greater than 15 x 10(9)/l strongly indicated a bacterial and, especially a pneumococcal aetiology, whereas increased S-LD could imply a mycoplasmal infection. For patients less than 50 years of age the equation C2 = -1.788 + 0.204 x WBC-0.0909 X S-LD was constructed, in which C2 greater than 0 indicated a pneumococcal aetiology. This function correctly classified 31/33 (93.9%) patients with a mycoplasmal and 20/31 (64.5%) patients with a pneumococcal infection. Patients with viral, Haemophilus influenzae or chlamydial infection could not be discriminated from each other. The age of the patient, WBC and possibly S-LD on admission are easily accessible parameters and these results could therefore be of value in daily clinical practice in hospitals.

Adolescent↗
PubMed1990

Minimal criteria for identification of Moraxella (Branhamella) catarrhalis.

A study was performed which aimed at testing the reliability of our routine diagnostic tests for identification of Moraxella (Branhamella) catarrhalis in clinical samples from the respiratory tract. A preliminary diagnosis of 122 isolates as Moraxella catarrhalis was obtained by using colony morphology and results of Gram stain and oxidase test as the sole diagnostic criteria. By using additional tests we could show that the preliminary diagnosis was incorrect for 21 isolates, which were classified as different Neisseria species. 20 of these were isolated from sputum samples. We propose that at least a test for DNA hydrolysis should be included in the routine procedure for identification of Moraxella catarrhalis in sputum.

Deoxyribonucleases↗
PubMed1989

Comparison of three methods for detection of pneumococcal antigen in sputum of patients with community-acquired pneumonia.

In a prospective study of 249 patients with community-acquired pneumonia, three tests for the detection of pneumococcal antigen in sputum were compared: a coagglutination test for detecting capsular antigens (Cap-CoA), a sandwich enzyme immunoassay (PnC-EIA) and a coagglutination test (PnC-CoA), both the latter detecting the pneumococcal C-polysaccharide common to all pneumococcal types. Sixty-three patients had culture-positive pneumococcal pneumonia, 45 pneumonia caused by other bacteria and 141 pneumonia of viral or unknown etiology. The sensitivity of Cap-CoA (63%) and PnC-CoA (65%) was somewhat higher than that of PnC-EIA (49%), but not significantly so. The specificity was 96-98% for all three methods. Using PnC-CoA 66 patients with possible pneumococcal infection were detected, the diagnosis being verified by culture in 41. Using Cap-CoA 59 such patients were detected, the diagnosis being verified in 40, and using the PnC-EIA 47 such patients were detected, the diagnosis being verified in 31. Antigen was found almost as often in non-purulent as in purulent samples, and as often in washed as in non-washed purulent samples. However, antibiotic treatment before the sputum sample was obtained resulted in significantly lower sensitivity of both PnC-CoA and Cap-CoA. This study confirms the high sensitivity and specificity of methods for pneumococcal antigen detection in sputum. Since CoA is easier and quicker to perform, and cheaper than the EIA, either PnC-CoA or Cap-CoA would seem to be the technique of choice for detection of pneumococcal antigen, whereby all sputum samples, including non-purulent samples, can be used.

Adolescent↗