Biomedical subjects

I Jakubicka

Publications and source records attributed to I Jakubicka.

4 recordsLinked to original sources

PubMed1991

[Detection of estrus and determination of the optimal time for insemination in polar foxes (Alopex lagopus)].

Oestrus cycles were investigated in seven polar foxes: the investigation consisted of evaluation of clinical symptoms of heat (vulva enlargement), measurement of vaginal mucus electrical resistance and determination of progesterone concentrations in the blood plasma of foxes; the objective was to determine optimum insemination time. The females were inseminated with fresh semen intravaginally by means of a pseudopenis, a day following the record of maximum electrical resistance of vaginal mucus and at the minimum progesterone concentration in blood plasma of 30 ng.ml-1. The females were reinseminated on the following day. The insemination dose had a volume of 1 ml with sperm concentrations of 150.10(6). Clinical symptoms of heat were observed in six out of the seven test foxes. Heat detection by means of measuring vaginal mucus electrical resistance was successful also in six females. Progesterone test enabled to detect heat in five females. Out of six inseminated females, five foxes became pregnant (83.3%) and a total of 33 cubs were born; this is 5.5 cubs per female.

Animals↗

PubMed1989

[Collection of sperm from foxes using electroejaculation under halothane anesthesia].

A method has been developed for the collection of ejaculate from anaesthesized dog foxes. General anaesthesia was produced by inhaling 3 to 5% narcotan mixed with oxygen, the inhalation rate being two litres per minute. An electroejaculator with a bipolar electrode, introduced in the rectum, was used for the electrostimulation of ejaculation. The voltage needed to produce the ejaculation effect and the number of impulses depended on the individuality of the foxes. The average amount of ejaculate per one collection was 0.86 ml, the sperm motility percentage was 74.80% sperm concentration was 270.62 x 10(3) spermatozoa per mm3, and the average pH value was 6.87.

Anesthesia, Inhalation↗

PubMed1989

[Fertilizing capacity of the ejaculate of nutria (Myocastor coypus) after the removal of the seminal vesicles as evaluated by the penetration test and natural mating].

The fertility of male coypu sperm following seminal vesicle extirpation was investigated using the penetration test into the egg of Syrian golden hamster (Mesocricetus auratus). Ejaculates were obtained from five males by means of electro-ejaculation under halothane narcosis. The results of the zona-free hamster eggs (ZFHE) penetration test showed that the ejaculates of all the surgically treated coypu males were fertile and that ZFHE value fluctuated from 54 to 76.6%. The results obtained in experiments with natural mating revealed that the extirpation of male coypu seminal vesicles did not affect their fertility. In total 47 foetuses were found post mortem in ten coypu females covered by surgically treated males, which on average represented 4.7 foetuses per female.

Animals↗

PubMed1989

[Fluctuations in the level of progesterone in the peripheral blood of nutria during pregnancy].

The fluctuations in the concentrations of progesterone in peripheral blood of three intact coypus and three coypus after natural mating were investigated for the period of four months. Immediately after mating the concentrations of progesterone are low (1.5-2.4 ng.ml-1), the maximum values reach the peak between the 14th and 15th week of gravidity (12.50-20.72 ng.ml-1), whilst there is almost no change in the intact females. On the last days of gravidity the values of progesterone decrease rapidly and they are very low on the day of parturition (1.0-2.5 ng.ml-1). The concentration of progesterone in the blood plasma of intact females in the test period is low (0.09-3.18 ng.ml-1).

Animals↗