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Biomedical subjects

I Julis

Publications and source records attributed to I Julis.

31 records · Page 2Linked to original sources
PubMed1990

[Histochemistry of selected peptidases in small intestine mucosa in piglets experimentally infected with Isospora suis].

In the small intestine mucosa of 24 gnotobiotical piglets experimentally infected the first day post partum with oocysts of the coccidium Isospora suis, the activities of dipeptidylpeptidase IV (EC.3.4.14.5.; DAP IV) and gamma-glutamyl transferase (EC.2.3.2.2.; GGT) in the microvillous zone of enterocytes were evaluated by scanning densitometry. The tissue of the small intestine in piglets infected with a dose of 100,000 oocysts of the coccidia of I. suis was examined in the period from the first till the eleventh day post infection (DPI). In the control piglets at the age of 2-5 days it was found that most of the DAP IV activity was located in the microvillous zone of the enterocytes of the middle jejunum, rear jejunum and ileum. The DAP IV activity of duodenum mucosa was lower; as compared with the activity in the mucosa of the jejunum and ileum it reached 53-57%. In the case of GGT activity, the highest density values of the reaction product were recorded in the microvillous zone of enterocytes of the duodenum and the whole jejunum, the lowest in the ileum mucosa (86-89%) of the activity found in the duodenum and jejunum). During the experimental infection the infected piglets had a significant deficit of both peptidases, especially DAP IV (the whole studied period). The development of GGT activity was slightly different with the onset of the marked decline of the enzyme activity only on the fifth DPI. The lower GGT activity persisted till the eighth DPI. The density of the GGT reaction product began to return to the normal on the ninth to eleventh DPI. No predisposition in the location of the deficit was observed in the peptidases studied during the infection. The decline of the activity of both enzymes influenced also the mucosa of all studied parts of the small intenstine. The difference lay in the relevance of lowering of the density of reaction product of DAP IV and GGT on other DPI and in the different intensities of the return of the activity to the physiological normal.

Animals↗
PubMed1990

[Alkaline phosphatase activity in goblet cells in the small intestine of piglets experimentally infected with the coccidium Isospora suis].

Alkaline phosphatase activity (EC. 3.1.3.1.) in goblet cells was investigated in the small intestine of 16 gnotobiotic piglets infected one day after delivery (DAD) by different rates of oocysts of Isospora suis coccidia. At a high infection rate of I. suis (750,000) the goblet cells were found to be highly positive to alkaline phosphatase on day 3 to day 4 after infection (DAI). In piglets infected by a low infection rate of I. suis oocysts (100,000) the activity of alkaline phosphatase activity in goblet cells was proved on days 4 to 10 after infection. In the first group of piglets, the positive goblet cells prevailed in the middle region of jejunum, with the peak on 4th DAI. It the second group of piglets a marked increase in the alkaline phosphatase activity was recorded in the goblet cells in the posterior part of jejunum on days 4 to 5 after infection and on 10th DAI. No alkaline phosphatase activity in the goblet cells was demonstrated in the control gnotobiotic piglets at the age of two to seven days.

Alkaline Phosphatase↗
PubMed1990

[Morphometric analysis of the activity of alkaline phosphatase in the small intestine of piglets infected with the coccidium Isospora suis].

Gnotobiotical one-day-old piglets were infected with 100,000 Isospora suis coccidia oocysts, and were immediately killed. In piglets killed on the 3rd to 11th day after infection (DAI), the morphometric analysis of alkaline phosphatase activity was performed in the area of the microvillus zone of small intestine. In the control 2-7 days old animals, the small intestine was not equally supplied with alkaline phosphatase. In duodenum the activity reached 88.37 per cent of the active length of absorbent surface (% LAac), in the middle jejunum 95.98 per cent LAac, in the dorsal jejunum 78.63% LAac and the ileum 90.55 % LAac. The width of the active area was more balanced and ranged from 5.003 microM in the ileum to 6.129 microM in the dorsal jejunum. In infected gnotobiotical piglets the lowest activity was found out on the 3rd to 4th DAI, with a greater decline on the 9th day after infection. The range from 25.99 to 40.50 per cent LAac with minimum in the duodenum and maximum in the ileum was observed on the 3rd DAI. In the middle and dorsal ileum the activity was nearly equal (28.34 and 27.69 per cent LAac). nI the dorsal jejunum a moderate increasing was up to 47.13% LAac on the 4th DAI, with the exception of the ileum, where the activity of alkaline phosphatase decreased to 24.96% LAac. On the 9th DAI the activity of alkaline phosphatase was nearly equal in the whole small intestine (from 55.70 to 60.01% LAac) with the maximum in the middle jejunum. In the width of the reaction product a direct dependence on the total activity of alkaline phosphatase was evident only in the segment of the middle and dorsal jejunum and ileum, but merely on the period of the 3rd to 4th DAI. The lowest values were measured in the middle jejunum (0.982 micron on the 3rd DAI and 0.709 micron on the 4th DAI). No dependence was observed between the total activity and the reaction product in the middle jejunum (0.982 micron on the 3rd DAI and 0.709 micron on the 4th DAI), there was no general stabilisation of the activity of alkaline phosphatase.

Alkaline Phosphatase↗
PubMed1986

Possible effects of muramyl dipeptide on liver cell membranes.

Three different approaches were utilized for illustration of membranous effect of muramyl dipeptide (MDP) on liver cells. Toxicologic approach using a hepatoprotective effect against CCL4 as well as from previous results with other toxins. Histochemistry and microfluorometry after chemical injury. Biochemical as assessed by modulation of 14C palmitic acid incorporation under MDP effects in neutral lipids and phospholipids content of cell membranes. By this integrated study we may assume MDP played a role in one or more cell membrane(s). This assumption was supported by reduction of hepatocyte injury as assessed by enzyme analysis in serum or in the cells. Moreover, MDP pretreatment influenced the increase in the incorporation of labeled palmitic acid in neutral lipids and phospholipids. Microscopical image analysis and histochemical results supported the aforementioned findings.

Acetylmuramyl-Alanyl-Isoglutamine↗
PubMed1986

DNA microfluorometry of synovial effusion cells in rheumatic diseases.

A microfluorometric study of relative DNA content in lymphocytes and granulocytes from the synovial fluid of 26 patients with rheumatoid arthritis or seronegative reactive arthritis was carried out. Results obtained from individuals without treatment or treated with nonsteroid anti-inflammatory drugs were compared with measurements of 7 healthy persons. DNA content in granulocytes was more sensitive to effects of disease or therapy than in lymphocytes. Introduction of therapy resulted in decreased percentage of cells in synthetizing or mitotic phase of the cell division cycle (microfluorometric index showing non G0 (G1 population]. A possible local influence of regulatory substances produced in the arthritic joint on DNA metabolism of both cells under study was suggested.

Anti-Inflammatory Agents, Non-Steroidal↗
PubMed1977

[Neuroectodermal tumors of the skin (a normative study)].

A group of bioptically examined neuroectodermal tumours of the skin and soft tissues consisting, in particular, of 170 Schwannomas and 350 tumours has been reevaluated in a retrospective study. The following common classification of these tumours has been recommended: I. Tumours of Schwann's cells: 1. neurilemmoma [A and B], 2. neurofibroma [with the following variants v. Recklinghausen's type, plexiform, pigmented, Paccinian, with Meissner-Wagner's bodies and meningiomatous], 3. amputation neuroma, 4. neurosarcoma, 5. others. II. Melanogenic tumours: A. pigmented naevi (junction, mixed, intradermal, epithelioid, clear cell, halo, neurocutaneous, fibrous, blue, proliferating blue, melanotic progonoma, others). -B. praecancerous melanosis. -C. malignant melanoblastoma (common type, from praecancerosis). III. Tumours of ganglion cells: 1. ganglioneuroma, 2. neuroblastoma, 3. paragangliomas (with granules, without granules, alveolar soft part sarcoma).

Ganglioneuroma↗
PubMed1975

Blastic leukemias affecting mammary glands.

Two clinical cases of blastic leukemia with tumorous infiltration of mammary gland are presented. Literature data and diagnostic problems of this type complication are discussed.

Adult↗
PubMed1975

Alveolar macrophage response to experimentally induced ornithosis.

Effects of two strains of the agent of ornithosis upon alveolar macrophages of mice were compared. Macrophages were obtained by lavages of the lower respiratory tract. Experimental mice were intranasally infected with 100 LD50 of strain P 89 and strain Stanglová, respectively. Up to the culmination of the disease (from 6th to 8th day in P 89 and from 5th to 8th day in Stanglová), the number of harvested macrophages increased. The phagocytic index soon reached values around 40% (P 89) and 25% (Stanglová). Basophilic and eosinophilic macrophages increased in volume and their nuclear as well as plasma membranes became disrupted. A tendency of alveolar macrophages to fuse and form syncytial elements and sporadically, rosets, was observed. All cells in the lavage went through the same changes. Histological examination has shown initial changes in epithelial cells of bronchi and development of pneumonia after fusion of peribronchial leucocytic infiltrates. By employing the above described technique, no great changes in the quality of the effects of either strain P 89 or Stanglová were found. A slight difference was observed only in the degree of alveolar macrophage stimulation and in the onset of symptoms.

Animals↗