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Biomedical subjects

I K Genefke

Publications and source records attributed to I K Genefke.

At least 37 records · Page 2Linked to original sources
PubMed1976

[The action of reserpine on the concentration of 5-hydroxytryptamine, epinephrine and norepinephrine in the venous blood of migraine patients].

Vasoactive amines, particularly 5-hydroxy-tryptamine (5-HT) have been implicated as the cause of migraine. Our study was a biochemical one on the effect of reserpine on the content of 5-HT, NE and E in migraneurs. The effect of one single infection of reserpine. 23 migrainous patients and 7 controls were given one i.m. injection of reserpine (1,5 mg/1.7 m2 surface). The content of 5-HT in blood platelets of 8 migraneurs and 7 controls 6 hours after the injection of reserpine decreased to comparable levels in both groups. The serotonin releasing effect of reserpine, however, is different from that of tyramine. The effect of prolonged reserpine medication. The effect of i.v. injection of reserpine over a period of 6 weeks on 5-HT, NE and E, was investigated. The doses of reserpine corresponded exactly to the doses already used by Nattero et al. (0.2 mg reserpine 3 times a week for 6 weeks). In correspondance with this work a double blind clinical examination was carried out by Nattero et al. (1975). Blood amine levels were measured weekly. The NE decreased to a minimum of 62% of basal mean value after 3-7 weeks of treatment. The decrease is significant from the third to the sixth week. Basal value was not reached until 6 weeks after withdrawal of reserpine. The concentration of 5-HT in blood platelets decreased to 5% of basal mean value and remained low during the reserpine treatment. The decrease is highly significant. A clinical improvement began 1-2 weeks after the introduction of reserpine treatment, continued during treatment and for 2-6 weeks after. In the open trial performed by us, we can confirm the results of Nattero et al. We demonstrated a marked decrease in concentration of blood amines corresponding to clinical improvement.

Epinephrine↗
PubMedDate not supplied

Electrical and thermal injuries in pig skin--evaluated and compared by light microscopy.

The morphology of pig skin after electrical (el) and thermal exposure was studied in order to find methods applicable for disclosing electrical torture. Biopsies from pig skin exposed 24 hours earlier to either heat or electrical current under general anesthesia were studied by light microscopy. The amount of energy used of either type ranged from about 6 to about 100 joule deposited on two circular areas of the skin measuring 12 mm in diameter. In sections stained with hematoxylin and eosin alterations were found in heat-influenced specimens following the administration of moderate and large doses of energy. Changes were observed in el-influenced specimens also after the administration of low amounts of energy, which did not lead to macroscopical changes in the skin. The two types of energy induced different patterns of alterations inside the epidermis. The most characteristic change following heat exposure was the presence of a granular or fibrillar eosinophilic cytoplasm and subepidermal clefts, while el-expoure often produced white homogeneous cytoplasm and shadowy nuclei ("white necrosis") in the attached epidermis. The individual changes could be produced by both types of injury, but to a highly different degree. However, the alteration classified as "vesicular nuclei" was only observed observed in el-damaged skin. The dermis from the more severely injured skin of both groups showed a homogeneous appearance ("necrosis"). While the changes of epidermis and dermis in response to heat were diffusely distributed, the changes created by electrical injury were often present in segments. By electrical damage homogeneous areas with loss of stainability and shadowy nuclei were occasionally observed in sweat glands, hair sheaths and in vessel walls, structures which are suggested to serve as conductors for the electrical current. "Vesicular nuclei" were observed in similar structures. Some of the findings may be associated with differences in intensity of energy during exposure to heat and electricity. Furthermore, the existence of different biological patterns following the two types of exposure should be considered. The studies are being continued to elucidate these basic questions, and to study further the medical diagnostic potential of examining skin biopsies from tortured individuals.

Animals↗
PubMedDate not supplied

Early epidermal changes in heat- and electrically injured pig skin. I. A light microscopic study.

In order to find methods applicable for disclosing electrical torture, pig skin was exposed to heat and electricity under controlled circumstances. Biopsies were obtained immediately after exposure and the morphology of the heat lesions was compared to that of electrical lesions. The cytoplasm of epidermal cells in heat lesions appeared granular or fibrillar, while the nuclei were rather unaffected. In electrical lesions the cytoplasm appeared homogeneous often with a peculiar white colour in hematoxylin-eosin stained sections. The nuclei were either dark and shadowy or enlarged and vesicular. The keratin in electrical lesions often showed a bright yellow colour. Small defects in the epidermis were seen in some electrical lesions. Thus the morphology of electrical lesions differed markedly from that of heat lesions. Although some of these differences may be due to differences in distribution and intensity of energy, it is probable that pH shifts in the cells due to electrolysis is the main cause of the specific morphology of electrical lesions.

Animals↗