[Vital activity of methane-oxidizing bacteria after their filtration through coal pore structures].
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Coal↗
Biomedical subjects
I K Kurdish
Publications and source records attributed to I K Kurdish.
[The interaction of Bradyrhizobium japonicum with clay minerals].
It is shown that such clay minerals as palygorskite and montmorillonite stimulate growth activity of Bradyrhizobium japonicum. The bacteria come into contact with the above minerals. Granulated preparations of rhizobia have been developed on the basis of the results obtained. These preparations are characterized by the high yield of viable cells and a possibility of long-term storage. The use of montmorillonite in production of granulated preparations provides the higher yield of viable bacteria in the preparations and stability of their composition under long-term storage.
Aluminum Silicates↗
[Characteristics of hemagglutination reaction in methanotrophic bacteria].
The reaction of hemagglutination with trypsin-treated rabbit erythrocytes was used to reveal lectins on the cell surface of methanotrophic bacteria and in their culture liquids. By this method, no lectins were detected on the cell surface of Methylococcus capsulatus IMV B-3001 and Methylomonas rubra IMV B-3075 or in the culture liquid of any of the species studied. With intact cells of Methylocystis parvus IMV B-3491, the positive hemagglutination reaction observed was nonspecific and most probably occurred due to the high cell surface hydrophobicity characteristic of this species.
Animals↗
[Effect of certain environmental factors on Bradyrhizobium japonicum].
Dependence of motility and chemotaxis was studied in two strains of Bradyrhizobium japonicum upon several environmental factors. In both strains, chemotaxis was found to increase with an increasing concentration of the attractant (glucose) to 5.6 x 10(-2) M. Both motility and chemotaxis reached their maximum in the two- to three-day cultures at neutral pH. The maximum motility of these bacteria occurred at 40 degrees C. The maximum values of chemotaxis in these microorganisms were, however, observed at 20-25 degrees C. Chemotaxis in acidic or alkaline media and at low temperatures was found to be markedly weaker. Nonoptimal values of these parameters in soil may be a limiting factor for the interaction of the given bacteria with soybean roots.
Bradyrhizobium↗
[Use of highly dispersed materials for culturing and isolation of granular Agrobacterium radiobacter preparations ].
The effects of synthetic and natural high-dispersion materials on the growth of Agrobacterium radiobacter were studied. Natural minerals montmorillonite and palygorskite (10 g/l nutrient medium) were more potent than high-dispersion silica and its modified forms in stimulating growth of Agrobacterium radiobacter. The interaction of Agrobacterium radiobacter with clay minerals increased the survival rate of bacteria at supraoptimal temperatures. We elaborated new granular bacterial preparation, which enhanced the productivity of cucumbers by 12-15%.
Bentonite↗
[New strains of soil bacilli which mineralize organic phosphorus compounds].
Microorganisms, which mineralize organophosphates (216 isolates) have been isolated for the chernozem soils of different regions of Ukraine. Sixteen most active strains referred to Bacillus genus have been identified and studied. These microorganisms grow (with different intensity) on the mineral medium with glucose and glycerophosphate or phytin, accumulating phosphate-ions in the medium. The most active strains mineralized glycerophosphate, accumulating about 280 micrograms/ml of PO4(3-) and phytin accumulating up to 130 micrograms/ml of PO4(3-) during 3 days. New strains of bacilli have been chosen for creation of combined bacterial preparations of the complex effect.
Bacillus↗
[Interaction of microorganisms with solids].
The paper generalizes data from literature dedicated to microorganisms interaction with solids. This interaction is widely spread in nature and considerably affects physiological activity of microbial populations. The role of physical, chemical and biological factors in this process is analyzed. It is noted that under certain conditions the hydrophobic forces are the basic factor in this interaction. The role of the cell surface components in this process is discussed.
Bacterial Adhesion↗
[Effect of gas mixture component composition on the process of hard materials colonization by methanotrophic bacteria].
A device has been proposed which allows decreasing a possibility to contaminate the inoculum of methane-using bacteria (methanotrophs) under its growing. The gas mixture composition has been investigated for the intensity of grass and polysterene colonization by two species of methanotrophs: Methylococcus capsulatus UCM B-3030, possessing hydrophilic surface and hydrophobic Methylocystis parvus UKM B-3490T. It has been shown that immobilized methanotrophic bacteria can colonize the above materials in the wide range of methane and oxygen concentrations in gas mixture. The process of hard materials colonization was most intensive when the mixture contained 17-28% of methane and 5-28% of oxygen. No essential differences have been registered under colonization of hydrophilic (glass) and hydrophobic (polysterene) materials by the both cultures.
Biofilms↗
[Effect of gas pressure on the physiological state of methylotropic bacteria].
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Gases↗
[Formation and function of the bean-rhizobium symbiosis in soy plants upon introduction of strains of Azotobacter and Bacillus species].
The effects of bacteria belonging to the genera Azotobacter and Bacillus in a mixed culture with Bradyrhizobium japonicum strains on formation and function of the legume-rhizobium symbiosis of soybean plants were studied. The data showed that the bacterial compositions B. japonicum 634b + B. subtilis 5, B. japonicum 634b + A. chroococcum 20, and B. japonicum 10k + A. vinelandii 56 with a cell ratio of 1:0.1 increased the number and weight of root nodules as well as the height and weight of the aboveground plant parts in almost all the cases by 22-105% compared with the control variants. These binary microbial cultures may be used for development of combined bacterial preparations for soybean.
Azotobacter↗
[Physiological activity of mixed cultures of Methylcoccus capsulatus UKM B-3030 with Bacillus megaterium UKM B-5723T and Bacillus subtilis VKPM B-1489 on solid surface colonization].
Physiological activity of monoculture of Methylococcus capsulatus UCM B-3030 and its mixed cultures with two bacilli species distinguished by proteolytical properties--Bacillus megaterium UCM B-5723T and Bacillus subtilis BK[symbol: see text]M B-4189 in terms of long-duration cultivation is investigated. It is shown, that the most active methane consumption by methanotrophic bacteria and their mixed cultures with bacilli under solid surface colonization occurred on the second-eighth day of cultivation. The obtained results permit one to evaluate intensity of CH4 oxidation process by typical microflora of some econiches (depleted coal mines, in particular).
Bacillus megaterium↗
[The chemotactic properties of Bradyrhizobium japonicum in the presence of natural fine-grained minerals].
The natural argillaceous minerals montmorillonite and palygorskite were found to enhance the motility of Bradyrhizobium japonicum cells and to slow down their chemotactic motion to glucose. The latter effect of the minerals is probably due to the adsorption of mineral particles on the cell surface and the blockade of the receptors that are responsible for the chemotactic behavior of the bacterium.
Bentonite↗
[Features of solid materials' colonization by pure and mixed cultures of methanotrophs].
The process of colonization of hydrophilic (glass) and hydrophobic (polysterene) carriers by pure cultures of methanotrophs Methylocystis parvus UCM B-3490T, Methylococcus capsulatus UCM B-3030, as well as by their cultures mixed with Bacillus megaterium UCM B 5723T and Pseudomonas putida VKPM B-4188 under the conditions efficient for methanotrophic bacteria. M. parvus demonstrated the highest intensity of this process on the above carriers owing to high hydrophobic cell surface. Both methanotrophs colonized the glass surface more quickly with formation of microcolonies on carriers after 6 days of incubation in pure and mixed cultures with B. megaterium. The number of bacilli on these carriers quickly decreased. In the mixed cultures with P. putida the glass and polysterene colonization intensity decreased, while the amount of pseudomonas on carriers increased.
Bacillus megaterium↗
[Biological properties of the phosphate-mobilizing Bacillus subtilis strain IMV V-7023].
Biological characteristics of a new phosphate-mobilizing bacillus strain are reported. Species-level identification of the strain was performed according to morphological, cultural, and biochemical characteristics and the sequence of the 16S rRNA gene. The strain was identified as Bacillus subtilis IMV V-7023 and displayed a very high ability to mobilize phosphorus from its sparingly soluble inorganic and organic compounds and the capability of synthesizing biologically active substances; in addition, the strain essentially suppressed the growth of phytopathogenic bacteria, micromycetes, and agents causing various diseases of vegetable, cereal, leguminous, and other plants. The strain Bacillus subtilis IMV V-7023 is promising for developing bacterial preparations for crop production.
Antibiosis↗
[Soil microflora over the closed Donbas mines].
Composition of soil air and microflora in different soil horizons (to 30 cm depth) over some closed Donbas coal mines has been studied. The intensive methane leakages have been found in some soil zones over the closed coal mines. This results in formation of explosive and inflammable gas mixtures. Methane content in soil gas mixtures could vary from trace amounts to 34 %. The essential decrease of oxygen (to 3-4%) and increasing (up to 5 %) of CO2 concentrations that evidences for active microbiological processes in such soils is marked in the latter case. Methanotrophic bacteria involving methane into biogenic cycle are widespread (up to 106 cells per 1 g) in methane-containing soils over the closed coal mines. Microorganisms of different physiological groups in the community with methanotrophic bacteria were found in the given technogenic econiche.
Coal Mining↗
[Interaction of some Pseudomonas genus representatives with high-dispersed silicon dioxide particles].
The effect of increase of a negative charge in the cell during contact interaction with silicon dioxide particles similar to those previously obtained for methanotrophic bacteria was observed in pseudomonads. The microelectrophoretic method application has allowed to find a capsule and consists of uncharged components in Pseudomonas batumici bacterial cells; the capsule thickness was determined by conditions of microorganisms cultivation. The effect of increase of a negative charge took place for the cells grown on the Kozer medium where they formed a capsule of smaller dimensions. It is supposed that the sorption of silicon dioxide particles on the bacterial surface initiates the conformational changes of porins. As a result, the quantity of the charged groups increases and thickness of the surface layer in which these groups are located also augments.
Bacterial Adhesion↗
[The effect of dispersed silicon dioxide--aerosil A-300--on the growth of Saccharomyces cerevisiae yeasts].
High-dispersed silicon dioxide--aerosil A-300 has been studied for its effect on the growth of yeast Saccharomyces cerevisiae. It is shown that at heterophase cultivation of these microorganisms under periodical conditions introduction of aerosil A-300 induces a decrease of the lag phase duration and an increase of the maximum specific growth rate and economic coefficient of the carbon substrate utilization. This high-dispersed material has exerted the similar effect under the conditions of chemostatic cultivation. Optimal parameters of this process are determined.
Culture Media↗
[Effect of cultivation conditions of Bacillus subtilis IMV B-7023 on surface properties of cells].
It has been established that the cells of Bacillus subtilis IMV B-7023 grown on the medium where peptone or calcium glycerophosphate served as the only source of carbon and energy have similar acid type of the surface and are characterized by a high negative charge even at low values of pH of the dispersion medium. This may be determined by the presence of phosphate groups, teichoic acids being their carriers in Gram-positive bacteria. Glucose addition to peptone or glycerophosphate leads to the change of the bacteria surface type, i.e., at low values of pH the cells have total positive charge, that allows to suppose availability of protein nature components in the surface layers of the cell wall. Bacteria grown in these conditions are also characterized by the higher degree of adhesion to inert carrier (glass).
Bacillus subtilis↗