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I Koch

Publications and source records attributed to I Koch.

66 records · Page 4Linked to original sources
PubMedDate not supplied

[Organization of the Pediatric Tumor Cell Bank of the Society of Pediatric Oncology and Hematology (GPOH)].

Characterized cell lines are absolutely necessary in applied research of cell biology and medicine. For the completion of diagnosis and therapy especially in pediatric oncology we are establishing a Cell Bank for Pediatric Tumors. The Cell Bank for Pediatric Tumors collects tissue samples of different types of solid malignant tumors from children and young adults. The specimens are transferred to in vitro culture (guidelines of the American Type Culture Collection-ATCC), the resulting cells are characterized to assure accordance with the histogenesis of the original tumor and stored in liquid nitrogen. The cell cultures are characterized morphologically (phase contrast microscopy) and immunocytochemically (ABC-method). To prove the malignancy of cells in primary culture the amount of hypertetraploid cells was determined (DNA-Scanning-Cytophotometry). Cell lines are checked to find out whether they develop tumors in nude mice followed by an analysis of the karyotype. Additional investigations (e.g. in vitro test of cytostatic drug resistance) are carried out on request by the sender. Part of the tumor tissue which is used to start the cell culture is in parallel diagnosed histopathologically at the Children's Tumor Register, Kiel and/or at the Charité. By the end of the year 1995 the Cell Bank for Pediatric Tumors had received 183 different specimens including 123 solid tumors (e.g. 24 neuroblastomas, 18 osteosarcomas, 12 Wilms' tumors, 13 rhabdomyosarcomas), 44 tissue specimens without any malignant cells, 8 probes without vital cells and 8 leukemias and lymphomas. We were able to establish primary cell cultures of 50% of the sterile tumor tissue probes, to cultivate them for a minimum of 5-10 passages, to characterize and freeze them. Six out of these tumor cell lines were already cultivated for one year and are available to the scientific community.

Adolescent↗
PubMedDate not supplied

Cyclophosphamide effect on changes in rabbit peripheral blood in the development period.

The effect of cyclophosphamide on peripheral blood changes in rabbits to the dose and duration of drug administration and the developmental age of the animal was studied. Cyclophosphamide was administered orally in an aqueous solution. The best tolerated dose was 16 mg/kg of body weight. One such dose decreased transiently the peripheral blood white cell count. The some dose administered during six consecutive days produced blood disturbances the more severe and long-lasting the younger were the animals used in the experiment. With higher cyclophosphamide doses blood changes were associated with signs of development arrest.

Administration, Oral↗
PubMedDate not supplied

[Mali children antipoliomyelitic immunity before and after two oral immunizations against poliomyelitis (author's transl)].

In tropical areas transport and storage of living antipoliomyelitic vaccine are difficult and consequently it is considered as hazardous to get a correct immunity with this vaccine. Still, good results have been obtained in Mali children with Sabin vaccine. A previous virological survey has shown that 31 p. 100 of children below 3 years carry enterovirus which are, for 30 p. 100 of them, wild poliovirus. In a serological survey conducted during the same period, near 50 p. 100 of the children in that age group were seronegative for the 3 types of virus and 90 p. 100 of the children between 4 to 7 had neutralizing antibodies against type I, 88 p. 100 against type II and 67 p. 100 against type III. By doubling or trebling the immunizing dose at the first vaccination, the following results have been observed after the second vaccination: 86 p. 100 of the immunized children under 3 years had neutralizing antipoliomyelitic antibodies (titre 1/10) against type I and II and 64 p. 100 against type III, whereas, in the elder group (from 4 to 7) the conversion rate is 100 p. 100 for types I and II and 96 p. 100 for type III.

Antibodies, Viral↗