Biomedical subjects
Publications and source records attributed to I Koseki.
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When Ehrlich ascites tumor cells fused with UV-inactivated HVJ (Sendai virus) were cultured, the viral envelope antigens, which had been integrated at random into the cell membranes, accumulated at one site on the fused cell. The site showed a specific structure, namely, a projection of the cytoplasm from the spherical fused cell surface with clusters of many long microvillilike protrusions from it. When the fused cells were conjugated with antibody against Ehrlich ascites tumor cells and cultured, the antigen-antibody complex also accumulated on the projection.Associated with the development of the projection and accumulation of viral antigens, vacuoles developed at the base of the projection in the cytoplasm of the fused cells in culture. The development of the projection and vacuoles and the accumulation of viral antigens were all inhibited by glucose, mannose, galactose, sodium azide, and cycloheximide, and these inhibitions were reversible. All these structures were observed in multinucleated cells, but few in mononucleated cells after reaction with HVJ (Sendai virus). We consider that these three phenomena correlate with each other and may result from changes in cell-membrane systems caused by fusion of cells.
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IB-RS-10-II subline with tetraploid level cells was more susceptible to the infection by the foot-and-mouth disease virus (FMDV) ASQ-PG strain than IB-RS-10-I subline with diploid level cells, when number and size of plaques and cytopathogenic effect of the virus were used as criteria. Besides, the virus yield in one-cycle of infection was almost the double in IB-RS-10-II than IB-RS-10-I cell subline and the near-tetraploid cells were more susceptible to be infected by the virus than the near-diploid cells. However, in relation to FMDV ASQ-Pp strain the cell subline with a diploid level was more susceptible than that with a tetraploid level. The effects of polyionic compounds under specific experimental conditions were peculiar to each cell subline. 1. Pretreatment of cell monolayers with DEAE-dextran increased the diploid level cells receptivity to the virus, whereas that of the tetraploid level cells was affected only slightly; pretreatment with dextran sulphate did not affect the receptivity of both cell sublines. 2. Pretreatment of the virus with the polycationic compound increased significantly its infectivity to the cells of both sublines; pretreatment of the virus with polyanionic compound decreased significantly its infectivity to the diploid level cells but did not affect that for the tetraploid level cells. 3. Treatment of the agar overlay with the polycationic compound induced increase on the plaque size formed in both cell sublines and treatment with the polyanionic compound inhibited plaque sizes in both cell sublines. A correlation was not found between the susceptibility of both cell sublines to the infection and the chromosomic alterations.
The influence of hypertonic medium on the relationship between two cell clones of IB-RS-2 swine line and the foot-and-mouth disease virus was studied. Although the number of infected cells was increased by the cell treatment with the hypertonic medium, before or during the time of virus adsorption and penetration onto the cells, showed by the plaque number, the viral replication was partially inhibited as showed by the plaque size as by the viral yield in one-cycle of infection. On the other hand, the cell treatment with that medium after virus adsorption and penetration time inhibited as the plaque number and size as the yield of the foot-and-mouth disease virus.
Cell cultures treated with tylosin tartrate and kanamycin sulphate antibiotics were studied in relation to the cell growth rate, the susceptibility to the foot-and-mouth disease virus and to the microorganism eradication. These treatments did not affect the cell growth rate and the cell behavior to the viral infection. On the other hand, the decontamination of the intracytoplasmatic formas of mycoplasma-like organism was not observed.
Morphology and chromosomes of cell sublines derived from two new bovine kidney lines are reported. Cell susceptibility to the foot-and-mouth disease virus is discussed. One of the sublines showed epithelial-like cells, while the remainder, elonged fibroblastic-like cells. Most of them had a diploid number of chromosomes. From these sublines only one was sensible to the foot-and-mouth disease virus.
The immune response to virus-infection-associated (VIA) antigen was studied in 379 Indian buffalos (Bubalus bubalis). These animals were vaccinated three times a year with commercial acetylethyleneimine (AEI)--inactivated foot-and-mouth disease vaccines under field conditions. Two months after the last vaccination, antibody against virus-infection-associated (VIA) antigen was found in 23 per cent. Foot-and-mouth disease virus -- (FMDV) type C "Waldmann" was isolated from oesophageal/pharyngeal fluid of 7 samples buffalos.
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Two swine kidney cell sublines, one of them IB-RS-10-I, with diploid level of chromosomes and the other, IB-RS-10-II, with tetraploid level, were studied as far as their morphology and karyotypic evolution was concerned. Both of them derived from the parental cell line after seven months in continuous culture and maintained in the same type of nutrient medium showed peculiar chromosome alterations for each subline, though in both sublines were observed losses of chromosomes belonging to the gruop GIV.
Virus-like particles resembling those seen in baby hamster kidney (BHK-21) cell cultures, CK-bovine cells, pig kidney cell lines PK-13 and IB-RS-2 have been found in the clones C-13 and C-26-3 of the cell line IB-RS-2. The particle's average outer diameter is c.85nm and the core is c.53nm
The growth rate and the protein level of two swine cell sublines, one of them with a diploid and the other with a tetraploied level of chromosomes, were studied during 96 hours at 37--38 degrees C. Both of them showed similar growth rates, although the cell number of the diploid cell subline had been higher than the tetraploid cell subline on the first observation at 24 hours. On the other hand the diploid cell subline showed lower protein level than the tetraploid cell subline.
Ultrastructural observation of Mycoplasma sp. revealed by the phosphotungstic acid-cromic acid, pH 0,4. Arq. Inst. Biol., São Paulo, 45(3) :169-176, 1978. Cells of IB-RS-2 (clone C-26-3) swine line were examined under electron microscopy, employing the differential coloration with PTA-ACr 5 %, pH 0,4, during 30 minutes, at 37 degrees C. Through this method we could observe the intracitoplasmatic forms, electronically dense, rather pleomorfic, with sizes varying between 60nm and 640nm and a unitary membrane of 90A similar to the extracellular forms described as Mycoplasma sp.