TinyGRAP database: a bioinformatics tool to mine G-protein-coupled receptor mutant data.
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Computational Biology↗
Biomedical subjects
I Kristiansen
Publications and source records attributed to I Kristiansen.
Antigen retrieval on epoxy sections based on tissue infiltration with a moderately increased amount of accelerator to detect immune complex deposits in glomerular tissue.
We wanted to examine the effect of antigen retrieval on epoxy sections where the tissue had been infiltrated by resin containing moderately increased amounts of accelerator. The concentration of accelerator DMP-30 (Tri(Dimethyl Amino Methyl) Phenol) was varied in the range of 0% to 4% in the infiltration step of the tissue processing. Some of the epoxy sections were fixed in osmium tetroxide, and for others this fixative was avoided. Immunogold labeling was performed on epoxy sections and LR-White sections of renal tissue with IgG-deposits, and the antibody used was anti-IgG. Antigen retrieval was performed by heating the sections in citrate buffer. The amount of immunogold labeling on retrieved sections increased according to the amount of accelerator the non-osmicated epoxy sections were based on in the infiltration steps. For the osmicated epoxy sections these differences were less pronounced. The immunogold labeling of retrieved epoxy sections was up to 70% of LR-White labeling. In addition to breaking fixation bond introduced by the chemical fixation, we believe that the antigen retrieval also breaks bonds between the epoxy resin and the embedded tissue. The combination of increased amount of accelerator in the tissue infiltration and antigen retrieval by heating the sections in citrate buffer is a good method for improving the immunolabeling of epoxy sections.
Animals↗
Ego Identity Status in cross-cultural context: a comparison of Norwegian and United States university students.
To examine patterns of identity development for late adolescents raised in the Norwegian mixed liberal welfare-state economic system compared with late adolescents raised in the free-market economic system of the United States, ego identity status scores and distributions were examined for 56 (37 women, 19 men). Norwegian and 1498 (814 women, 684 men) United States undergraduate university students using the Extended Objective Measure of Ego Identity Status-2. The United States sample was drawn from four geographic regions and comprised of those who had participated in prior studies performed by Adams. Significant differences were found between the two nations on all identity status subscales in the ideological and interpersonal domains for each sex. The more moderate identity status scale scores evidenced by the Norwegian sample may reflect a cultural trend toward greater moderation in the exploration and commitment process.
Cross-Cultural Comparison↗