Biomedical subjects
I Ling
Publications and source records attributed to I Ling.
S-antigens and isozymes in strains of Plasmodium falciparum.
Evidence was found for the independent reassortment of parasite genes for S-antigens and isozymes. The Lagos and Palo Alto strains of Plasmodium falciparum had the same isozyme forms of PGD and LDH but different S-antigens. In contrast, the BW (Gambia) and Palo Alto strains had different isozyme forms of GDH but shared some S-antigens. From artificial mixtures of parasites with different isozymes of GDH and different S-antigens, clones were derived in vitro which had a single isozyme type of GDH and a single type of S-antigen.
Fractionation and characterization of Plasmodium falciparum antigens.
In human malarial infections, the immunological importance of modified host cell components and of parasite antigens that may be released on spontaneous rupture of infected erythrocytes in vivo is not known. It is difficult, however, to analyse antigens extracted from entire parasitized red cells because of contamination with normal red cell constituents, notably haemoglobin. To overcome this problem, a simple chromatographic procedure has been developed that concentrates antigens and radiolabelled parasite products in a separate fraction from haemoglobin. Details are also given of investigations of two different kinds of antigen associated with Plasmodium falciparum. One antigen known to be immunogenic in man is shown to be associated with membranes. The other antigen is poorly immunogenic in man and other animals and is shown to be a soluble, rather stable, acidic protein.