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Biomedical subjects

I M Aref'ev

Publications and source records attributed to I M Aref'ev.

At least 19 recordsLinked to original sources
PubMed1988

[Toxicologic evaluation of individual chemical compounds and complex mixtures using a motile cell test object].

The possibility of quantitative prognosis of chemical compounds and complex mixtures toxicity by means of a biotechnical system with suspension of bull spermatozoa sensor was investigated. The concentration of some chemical compounds, inhibiting by half spermatozoa motility has been experimentally determined. A correlation between the values determined and acute toxicity of the compounds tested has been established. The possible use of the biotechnical system for the screening of medical polymer materials has been demonstrated.

Animals↗
PubMed1985

[Changes in the rate of surface blood microcirculation studied by photon-correlation spectroscopy in the practical treatment and diagnosis of diabetes mellitus].

The paper is concerned with the results of studies on the basal blood flow and responses of the microcirculatory channel to physiological and pharmacological loads in health and diabetes mellitus using the method of photon-correlation spectroscopy in the light fiber variant. The rate of the basal blood flow in patients with diabetes mellitus (irrespective of the diabetes type) as compared to that in health was significantly lower in the finger tip and tended toward reduction in the inner side of the forearm, lobule of the auricle and in the conjunctiva. The presence of retinopathies in patients with diabetes mellitus resulted in a significant decrease in the rate of the blood flow in the conjunctiva as compared to those in health and diabetes mellitus without retinopathy. The response of the blood flow to physiological (orthostatic and ischemic) and pharmacological (nitroglycerin and pentoxifyllin) tests in patients with diabetes mellitus was also low; the presence of angiopathies caused a more acute reduction of the reactive power of the microcirculatory channel to the orthostatic test. The nature of the nitroglycerin effect in health and diabetes mellitus was different: in the first case noticeable vascular reactions were observed, in the second case a persistent vasodilative effect was observed. The pentoxifyllin effect was characterized by an elevated rate of the blood flow in health and diabetes mellitus.

Adolescent↗
PubMed1984

[Evaluation of the photon correlation method of measuring skin blood flow and its use in the diagnosis of microcirculation disorders in patients with diabetes mellitus].

A new photon-correlation method for measuring blood flow and traditional methods for blood flow measurements according to the clearance of 133Xe were studied and compared. The measurement data of the skin circulation by the photon-correlation method and according to the clearance of 133Xe are in linear relationship, the correlation ratio being equal to +0.93 (P less than 0.01). At the same time the photon-correlation method has some advantages. Particularly it is marked by non-invasiveness, high accuracy, good reproducibility of the data and short exposure time. Photon-correlation spectroscopy was used to perform clinical studies of the skin circulation in different anatomic areas of the skin in diabetes mellitus patients. Altogether 117 subjects aged 19-60 years were examined. There were 38 healthy subjects and 79 patients with diabetes mellitus. The skin circulation was measured from the finger tip and from the internal surface of the upper third of the forearm. In healthy subjects, the skin circulation appeared substantially higher in the finger tip than in the forearm. In diabetes mellitus patients, the circulation in the finger skin was also higher than in the skin of the forearm. As compared to healthy subjects, the patients manifested a significant lowering in the finger circulation and only the tendency towards its decrease in the forearm.

Adult↗
PubMed1983

[Study of the kinetics of antigen-antibody reaction by light scattering].

Laser light scattering in conjunction with measurement of the spectral width and integral intensity of light scattering was applied to studying the process of complex formation of antigens and antibodies. The system of polysaccharide group A streptococcus and antibody against it was examined under varying polysaccharide concentrations. The measurements were performed every 10 s for 70 min after combining polysaccharide and antibody solutions. Within the entire time interval, the size of the complexes were less than the wave length of exciting laser light (0.633 m). This made it possible to determine their average magnitude in terms of the Rayleigh model.

Animals↗
PubMed1981

[Technic of optical mixing spectroscopy in the diagnosis of blood microcirculations].

Optical mixing spectroscopy was used to measure the correlation function of helium-neon laser light (wavelength 0.633 micron) scattered in the finger skin. The mean blood flow velocity was evaluated from the data obtained. It was shown that application of the method to the diagnosis of blood microcirculations demands that vascular reactions of laser light be taken into consideration. In most cases the mean blood velocity was observed to rise 1.5-3-fold within the first-second minute of irradiation at 0.6 W/cm2.

Humans↗
PubMed1979

[Detection of antigen-antibody reactions using optical beat spectroscopy].

The light beating spectroscopy technique was used for recording the results of serological reactions without visual phenomena. The investigated systems consisted of the antigens of influenza virus or Mycoplasma pneumoniae and the corresponding sera. A principal possibility of detecting the antigen-antibody complexes in serological reactions without visual phenomena by the light beating spectroscopy technique was demonstrated.

Antigen-Antibody Complex↗
PubMed1978

[Determination of sperm activity by the light scattering method].

A possibility of determination of sperm activity on light scattering spectra was studied. The laser homodyne light beating spectrometer was used for the measurements. Bovine sperm solutions were investigated. As shown, the sperm activity defined by spermatozoa motility could be determined through light scattering spectrum half-width. The advantages of the new method as compared with routine method of sperm activity determination are discussed.

Animals↗
PubMed1978

[Use of the light-dispersion spectroscopy for detection of the precipitation reaction].

The light beating spectroscopy technique was used to detect the precipitation test. The system consisting of human serum globulin--rabbit antiserum against human globulins was studied. A possibility of detecting the precipitation test for much more diluted systems of the antigen and antibody by means of the light beating technique than by the routine visual method was demonstrated.

Animals↗
PubMedDate not supplied

[A flow laser analyzer of erythrocyte deformability].

The analyser of erythrocyte deformation has been worked out, it allows one to obtain the distribution of the cells according to the deformation degree. The analyser consists of optic electronic block, sample insert unit, signal transformation unit and the information processing unit. The analyser is based on the principle of the small angle light dispersion of laser radiation registration made on each erythrocyte of the population, which was extended due to the influence of the shift efforts when the cell is passing through the narrow measuring capillary. The analyser was tested with the suspension model, normal and pathological blood samples. The analyser can be used for diagnosis and treatment of diseases related to the rheological blood properties.

Equipment and Supplies↗