Early pathogenesis and pathology of Tritrichomonas foetus infection in virgin heifers.
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Biomedical subjects
Publications and source records attributed to I M Parsonson.
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Two intramuscular injections of cloprostenol were given 12 days apart to synchronise oestrus in a group of 47 heifers and following the second injection, 38 were found in oestrus in 2 to 6 days, 1 in 11 days and 8 in 22 to 27 days. The conception rate of the synchronised animals was 24 of 38 (63%). Twenty-nine heifers were given injections of cloprostenol in late pregnancy and parturition was induced with a latent period of 6 +/- 3 days when injected at 266 to 267 days. When injected at 254 to 262 days, 3 of 16 heifers (19%) were unresponsive and the remainder calved with a latent period of 12 +/- 6 days. The survival rate of calves from induced heifers was 71% with 80% survival from controls. Approximately 50% of the induced heifers, and none of the controls, retained their foetal membranes.
A vaccine prepared from Campylobacter fetus subsp. fetus biotype venerealis gave only partial protection to heifers and bulls against genital infection with C. fetus subsp. fetus biotype intermedius. Heifers and bulls were fully immunised against C. fetus subsp. venerealis biotype intermedius by administration of a vaccine prepared from homologous organisms.
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Temporary contamination of the penis and prepuce with Campylobacter fetus subsp. venerealis and Campylobacter fetus subsp. venerealis biotype intermedius may occur when immunised bulls mate with infected heifers. However, 2 experiments showed that this contamination was not a significant cause of genital infection in susceptible heifers that subsequently mated with bulls.
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Ten cows and heifers (Group B) were inoculated into the uterus at oestrus with semen followed by IBR virus for the first insemination and semen alone if a second insemination was necessary. All animals developed infectious pustular vulvo-vaginitis (IPV), and 2 cows conceived to the first and 2 to the second insemination (pregnancy rate of 40 percent requiring 4.5 services per conception). This group was compared with 10 control animals (Group A) which were treated similarly but received tissue culture fluid instead of virus at the first insemination. Group A had a pregnancy rate of 90 percent requiring 1.7 services per conception. Natural mating of 4 bulls with preputial infections due to infectious bovine rhinotracheitis (IBR) virus with 9 susceptible cows and heifers (group D), resulted in the production of lesions of IPV. The IPV infection did not affect their fertility (pregnancy rate of 89 percent requiring 1.4 services per conception) when it was compared to a similar group of females (group C) mated to the same bulls prior to infection with IBR virus (pregnancy rate of 100 percent requiring 1.2 services per conception). The 6 animals in Group B that were not pregnant and returned to oestrus 3 times were found on slaughter to have endometritis, salpingitis and vaginitis. A high incidence, 5 out of 18 (28 percent), of shortened oestrous cycles (less than 18 days) was a feature of the breeding pattern of this group. The undesirable consequences of distributing semen contaminated with IBR virus from artificial insemination centres are apparent.
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