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I Málovics

Publications and source records attributed to I Málovics.

12 recordsLinked to original sources
PubMed1978

Heat-stable enterotoxin produced by Shigella flexneri.

Filtrates and ultrasonics extracts of Shigella flexneri showed rapid permeability factor (PF) test and proved positive in suckling mice and ligated rabbit loop tests within 4 hr. Delayed PF was not detected and the rabbit loop dilatation test read after 18 to 24 hr, the mouse pad oedema reaction, the test for elongation effect of CHO cells were also negative. In the delayed PF test a strong "blanching" effect was observed. A filtrate of an Ent-Escherichia coli strain was positive only in the rapid PF test, while filtrate and ultrasonic extract prepared from the Ent+ E. coli strain showed a positive reaction in all tests for enterotoxins (ST and LT) including the rapid PF test. Ultrasonic extracts of a S. flexneri and an Ent- E. coli strain concentrated by freeze-drying were fractionated on Sephadex G-100 column. S. flexneri fractions of 60--70ml were positive for rapid PF, dilation capacity in suckling mice, and the blanching effect in the delayed PF test. No positive reaction was found in the delayed PF test and in CHO cell culture. Similar fractions of Ent- E. Coli carried substances responsible for the rapid PF and the blanching effect, but without suckling mice positivity.

Bacterial Toxins↗
PubMed1978

Unique features of heat-stable enterotoxin of Shigella flexneri.

Sephadex G-100 fractions of ultrasonic lysate of Shigella felxneri were compared to the fractions of Escherichia coli lysates of Ent- , LT+ ST+, LT+ and ST+ strains. The range of molecular weight of S. flexneri ST fractions was the same as that of E. coli LT fractions. Rapid PF activity was associated with the ST peak in the case of S. flexneri, and followed the LT activity in the E. coli (LT+ ST+) fractions, and appeared in the same molecular weight range in the case of Ent- E. coli lysate. Cross neutralization could be demonstrated between S. flexneri ST and E. coli LT. Antigenic relationship between shigella ST and choleragen seemed to be less expressed and rather unilateral.

Animals↗
PubMed1977

Pathogenic effect of enterotoxigenic Escherichia coli and Escherichia coli causing infantile diarrhoea.

Macroscopic, light and electron microscopic alterations in ligated rabbit intestinal loops challenged with five standard enterotoxigenic Escherichia coli (ETEC) and twenty-three enteropathogenic E. coli (EEC-I) strains, freshly isolated from infantile enteritis cases, were investigated. Only two O26 : K60 : H11 strains produced enterotoxin. Their living cultures, sterile filtrates of the fluid medium and ultrasonic lysates of the bacteria resulted in pronounced hypersecretion of the intestinal epithelium followed by fluid accumulation and loop dilatation. These two E. coli strains, similarly as the other loop-negative EEC-I strains, were able to penetrate into the intestinal epithelium. In contrast to the standard ETEC strains, the EEC-I bacteria, adhering to the brush border, intruded into the microvilli, multiplied on the outer epithelial cell membrane making close contact with it and, causing, shedding of microvilli, penetrated into enterocytes becoming enclosed in membrane-bound phagosome-like vacuoles, appeared in the lamina propria and elicited mild focal polymorphonuclear infiltration.

Animals↗
PubMedDate not supplied

[Significance of postoperative fever after hip prosthesis implantation].

INTRODUCTION: Postoperative fever is a phenomenon well-known by every orthopedic surgeon, yet its etiological background and its relation to wound infections is still unclear. The number of publications related to this topics in general and in the special field of orthopedics is quite limited. METHOD: The incidence of postoperative fever in 177 patients who underwent total hip replacement was investigated; its nature and connections to possible bacterial wound infections are described. Moreover, the effectiveness of antibiotic therapy was also taken into consideration. RESULTS: In 39% of the sample, fever exceeding 38 degrees C was noted in the immediate postoperative period; in 87% of these cases, the fever was of unknown etiology, its background could not be specified by clinical tests and examinations. In four cases of the total 177 patients, microbiologically proven wound infection occurred during the period of hospital stay, but only three of these patients had fever. The results of patients treated with antibiotics because of fever did not differ significantly from those of patients who did not receive antibiotics. This was true from the aspect of both the disappearance of fever and the frequency of complications. CONCLUSION: The results emphasise that postoperative fever is only a minor predictive factor of wound infections; at the same time absence of fever does not mean absence of septic complications. In the case of immediate postoperative fever of unknown etiology and without clinical symptoms, it is inexpedient to start antibiotic treatment or to increase the prophylactic antibiotic dosage.

Adult↗