Biomedical subjects

I Menessy

Publications and source records attributed to I Menessy.

3 recordsLinked to original sources

PubMed1983

Decrease of red blood cell filterability seen in intensive care I. The correlation of low erythrocyte filterability with mortality and its return to normal values in critically ill patients under parenteral nutrition.

Damage to the internal viscosity of red cell is correlated with changes in the surface area to volume ratio and to prelytic phenomena. Considerable changes in shape were noticed in red cells from shocked dogs and some critically ill patients in blood smear or fresh preparation, or after in vitro treatment with colloidal plasma substitutes or glucose, and so erythrocyte filterability was investigated in critically ill patients, given or not given parenteral nutrition. The results revealed an increase of the erythrocyte filtration time (EFT 1/2) in more than 50% of the critically ill patients and less alteration in critically ill patients given total parenteral nutrition, the mortality being half as great. The return of increased EFT 1/2 to normal values occurred after 3-12 days of total parenteral nutrition in 6 patients investigated. A death rate twice as great was seen in patients with high EFT 1/2 as compared to the 27.7% mortality in patients with normal EFT 1/2, its correlation with increased filtration time being significant, r = 0.81 greater than 3 Sr, y = 1.291x + 27.44, n = 47.

Animals↗

PubMed1983

Decrease of red blood cell filterability seen in intensive care. II. Red blood cell crenelation "in vivo" as morphological evidence of increased red blood cell viscosity in low flow states.

We have reported the finding of numerous spiculed erythrocytes in blood stored for 15-21 days with acid citrate dextrose, drawing attention to the possible decrease of stored red cell deformability because of the decrease of the surface to volume ratio. The presence of anisotropy with crenated spheres, equivalent to the internal crystallization of hemoglobin, is closely related to these altered red cell parameters. The increase of erythrocyte filtration time in blood stored with acid citrate dextrose correlated well with the duration of storage up to the 21st day, r = 0.74 greater than 3 Sr, y = 1.104x + 1.853, n = 47, as well as with the increase of the proportion of crenated red cells. Using higher magnification (X700-1000) of the microcirculation in the great omentum of shocked dogs, crenated spheres could be seen within the arterioles, venules and capillaries; during refractory shock, nearly all the red cells became crenated spheres. The observation of echinocytes "in vivo" is a morphological proof of the damage to deformability of normal red blood cells in low flow states.

Animals↗

PubMed1980

Intrahematic crystallization of hemoglobin related to blood preservation with acid citrate dextrose and shock.

In 480 investigations and 40 samples of blood stored liquid with acid citrate dextrose, under adequate conditions for morphological study, many crenated cells of echinocyte types II-III were observed. In 17 samples of blood within 17-21 days of preservation 80-100% crenated red cells were observed including spherocytes. The filterability test performed with significant differences of P < 0.001. In 45 critically ill patients filterability was increased by more than 50%, P < 0.001. Some samples of stored blood appear anisotropic in polarized light microscopy, proving internal crystallization of hemoglobin, the only compound in the blood from some patients in shock and following massive transfusion of 3.5-4 liters. The phenomenon, undoubtedly could give evidence for the participation of internal crystallization of hemoglobin in the late stages of transformation of red cells into crenated shape with two possible factors being incriminated: the participation of membrane or crystallization of Hb. Anisotropic, birefringent, red cells indicate great modification in the specific viscosity of erythrocytes in above conditions. The data are in keeping with the findings of Taube, Massuda and Shoemaker (1973) who suggested that changes in the viscosity of the blood in traumatized patients may be related to alterations in the internal viscosity of the red cell.

Blood Preservation↗