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Biomedical subjects

I Miler

Publications and source records attributed to I Miler.

At least 55 records · Page 3Linked to original sources
PubMed1980

The suppressive effect of continuous infusion of bilirubin on the immune response in mice.

Mice (strains Balb/c and A/J) received an intravenous infusion of bilirubin for a 1 d period. The infusion was delivered at various phases of the primary reaction; the degree of the immune response was expressed as the number of antibody-forming cells against sheep erythrocytes. Bilirubin infusion during both the inductive and productive phase of the primary reaction decreased significantly the immune response. We assume that bilirubin influences the differentiation of immunocompetent cells immediately after their contact with the antigen; in addition it acts in the period of the quantitative increase of the number of antibody-producing cells.

Animals↗
PubMed1980

Preparation of zymosan from yeast cell walls.

Cell walls of the yeast Saccharomyces cerevisiae after disintegration and protoplasm removal by centrifugation and repeated washing were suspended in 0.5 M Na2HPO4, pH 7.8-8.0, as a 5% or 10% suspension, depending on the mode of heating. The suspension was boiled for 3 h, purified by repeated washing with water and ethanol and dried. The yield was approximately 1.8% of the starting amount of pressed commercial baker's yeast.

Cell Wall↗
PubMed1979

The decreased in vitro chemotactic activity of polymorphonuclear leukocytes of newborns and infants.

Using Boyden's technique, a statistically significant decrease in the chemotactic activity of polymorphonuclear (PMN) leukocytes was found during the early postnatal period, i.e. in the cord blood and in blood of newborns within the first 10-15 d of life after stimulation of cells with both zymosan-activated adult serum (ZAS) and with an abacterial filtrate of Escherichia coli broth culture (ECF). After this period, the responsiveness of leukocytes to both chemotactic agents increased and remained at the same level during the whole observation period, i.e. up to the age of 6 months. Nevertheless even then it did not reach fully the responsiveness of the leukocytes of mothers and pregnant women. Zymosan-activated serum was shown to be a more potent chemotactic stimulus to leukocytes of infants as compared to the E. coli filtrate.

Adult↗
PubMed1979

Skin-window study on the migration of leukocytes of newborns and infants.

Migration of leukocytes of newborns and of infants up to the age of 6 months was studied using the in vivo skin-window technique according to Rebuck. Using the non-specific stimulation (abrasion of the skin only) a slight age-dependent physiological increase of migration of cells was observed within the observation period; after a strong local irritation with diphtheria-tetanus-pertussis vaccine (Alditepera) there was a vigorous migratory response of cells in the skin lesion. Both quantitatively and qualitatively, the migration pattern (i.e. shift from PMN leukocyte to mononuclear cells in the exudate within a 1 d period after abrasion) was not influenced by immunization of infants with Alditepera, suggesting thus the nonspecific nature of this cellular response. The "normal" values of the chemotactic response of leukocytes of newborns and infants are given as a basis for evaluation of defects of this functional activity of leukocytes.

Age Factors↗
PubMed1979

The bactericidal activity of sera of healthy neonates and of newborns with hyperbilirubinaemia to Escherichia coli.

Cord and newborn sera were found to possess a significantly decreased in vitro bactericidal activity to Escherichia coli as compared to sera of adult individuals. Hyperbilirubinaemia in neonates suffering from both Rh and ABO incompatibility results in a further significant impairment of the bactericidal activity of their sera in comparison with healthy newborn infants. Exchange transfusion improved considerably the serum bactericidal activity. The possible mechanism of toxic action of unconjugated bilirubin on cells of the immune system is discussed.

Adult↗
PubMed1979

The bactericidal activity of sera of healthy neonates and newborns with hyperbilirubinaemia to E. coli.

Cord and newborn sera were found to possess a significantly decreased in vitro bactericidal activity to E. coli as compared to sera of adults. Hyperbilirubinaemia in neonataes suffering from both Rh and ABO incompatibility results in a further significant impairment of bactericidal activity of their sera in comparison to healthy newborn infants. Exchange transfusion considerably improved this serum antibacterial activity. The possible mechanism of toxic action of unconjugated bilirubin on the cells of the immune system is discussed.

Adolescent↗