PubMed Health⌕ Search

Biomedical subjects

I Molnár

Publications and source records attributed to I Molnár.

At least 55 records · Page 3Linked to original sources
PubMed1992

Evaluation of thyroid function and anti-thyroid autoantibodies in systemic sclerosis.

Parameters of thyroid metabolism, and the presence of anti-thyroid antibodies were investigated in 43 patients with systemic sclerosis. Anti-thyroid antibodies were detected in 14 cases. Elevated levels of anti-thyroglobulin antibodies were determined in 4 cases, anti-thyroid peroxidase (TPO) antibodies in 11, and anti-microsomal antibodies in 5. The detection of anti-TPO antibodies gave the most remarkable information about the presence of autoimmune thyroiditis. The patients with anti-TPO and/or reduced T3 concentration tended to have secondary Sjögren's syndrome. Our results provide further evidence that anti-thyroid antibodies might be responsible for the remarkable appearance of autoimmune thyroiditis in systemic sclerosis.

Adult↗
PubMed1992

The relationship between anti-human eye muscle antibodies and thyroid function, anti-TSH receptor antibodies and eye symptoms in Graves' ophthalmopathy.

Circulating IgG, IgA, and IgM antibodies to human eye muscle cytosol antigens were studied in 60 patients with Graves' ophthalmopathy using the indirect ELISA method. There was a significant difference in the levels of both IgG and IgA antibodies between the patients with Graves' ophthalmopathy and a control group (p < 0.001). IgA antibodies to eye muscle cytosol antigens were raised in 20 out of 29 patients with proptosis (class 3 ophthalmopathy), in comparison with 31 patients out of the total group of 60 with Graves' ophthalmopathy (p < 0.02). Anti-TSH receptor antibodies (TRAK) were not present in over half of the 31 patients with raised IgA antibodies to eye muscle antigens. However, a significant difference was found between the levels of IgG and IgA antibodies in the TRAK-negative patients (p < 0.05). These findings suggests that both IgG and IgA antibodies to eye muscle antigens might be important in the development of ophthalmopathy.

Adult↗
PubMed1991

[The role of antibodies in Graves' ophthalmopathy].

The aim of this study was to investigate the possible pathogenetic role of autoantibodies to eye muscle membrane fractions in 75 patients with Graves' disease (50 patients with infiltrative ophthalmopathy). Autoantibodies directed to four fractions of human eye muscle--as well as thyroid membranes prepared by ultracentrifugation were detected by ELISA and Western blotting techniques. None of autoantibodies to various fractions of eye muscle membrane proved to be specific for Graves' ophthalmopathy, however, the presence of these antibodies and activity of eye symptoms were in correlation. Polyclonal autoantibodies to common epitopes on both thyroid and eye muscle membrane antigens (approximately 60 kD) were revealed that might be involved into pathogenesis of Graves' ophthalmopathy.

Adult↗
PubMed1991

Comparative study on IgG and IgA antibodies against human thyroid and eye-muscle antigens in Graves' ophthalmopathy.

Circulating IgG and IgA anti-thyroid and anti-eye muscle antibodies were investigated in 87 patients with Graves' disease (60 cases with ophthalmopathy). The ELISA method was used. Both IgG and IgA antibodies were demonstrated against human thyroid and eye-muscle membrane or cytosol antigens. Anti-eye-muscle antibodies of the IgA type were observed more frequently than those of the IgG type (25 cases vs. 18 were demonstrated with membrane antigens and 37 cases vs. 23 with cytosol antigens). The respective distributions for thyroid antigens the cytosol fraction were 55 cases vs. 13 and 18 cases vs. 36. A significant difference was observed in the anti-thyroid IgG levels and the anti-eye-muscle membrane or cytosol levels between the patients with Graves' disease and those in control group (P less than 0.001). The difference in the IgA antibody to thyroid and eye-muscle antigens was significant between the patients with and without ophthalmopathy (P less than 0.002). The strong correlation between the levels of IgA antibodies to thyroid and those to the eye-muscle cytosol fractions might be connected with the theory of the common aetiology of the thyroid and eye diseases in Graves' ophthalmopathy (P less than 0.001). Circulating IgA anti-human thyroid and eye-muscle antibodies seemed to have a diagnostic relevance in the development of ophthalmopathy in Graves' ophthalmopathy.

Adult↗
PubMed1990

Cellular and humoral autoimmune responses against human eye muscle membrane antigen in Graves' disease.

75 patients with Graves' disease (54 with ophthalmopathy) were investigated using the tests of leucocyte adherence inhibition and immune adsorption with 125I-labelled Staphylococcus Protein A, against human eye muscle "crude" membrane antigen. The results of positive leucocyte adherence inhibition (10 out of 26 vs. 1 out of 28, P less than 0.05) and anti-human eye muscle membrane antibody index (mean +/- S.D.) (1.89 +/- 1.20 vs. 0.84 +/- 0.38, P less than 0.001) showed a correlation with the patients with clinically active eye disease and the HLA-B8 antigen in Graves' ophthalmopathy (P less than 0.01). Positive leucocyte adherence inhibition was observed in 9 out of 21 cases of Graves' disease without ophthalmopathy, but its prognostic relevance has to be confirmed in the development of ophthalmopathy.

Antibody Formation↗
PubMed1985

Morphine inhibits the carrageenan-induced oedema and the chemoluminescence of leucocytes stimulated by zymosan.

Morphine inhibited the oedema formation induced by carrageenan. The anti-inflammatory activity developed 120 min after carrageenan injection, suggesting that inhibition of the kinin phase might be partly responsible. This assumption is supported by the findings that morphine inhibited bradykinin oedema but did not influence oedema formation induced by histamine, 5-HT or PGE2. The anti-inflammatory activity of morphine was partially inhibited by naloxone (0.5-1 mg kg-1) in the carrageenan oedema test. Zymosan-stimulated chemoluminescence of neutrophils of the rat was inhibited both by morphine (0.1-10 microM) and naloxone (1-100 microM). When morphine and naloxone were administered simultaneously (10 microM) their inhibitory effects were additive. Naloxone also failed to antagonize the inhibitory action of morphine in lower dose (0.1 microM). These results suggest that the effect of morphine in inflammation might be mediated either by one of the opiate receptor subtypes insensitive to naloxone or a non-opiate mechanism might be involved.

Animals↗
PubMed1985

Antiinflammatory effect of the antioxidant MTDQ-DS.

The effects of native and oxidized forms of the new antioxidant drug MTDQ-DS (HUMAN, Hungary) on some parameters of acute inflammatory processes were studied. In short-term in vitro experiments, both forms at 10(-4) to 10(-6) g/l inhibited dose-dependently the zymosan-induced chemotaxis of the rat peritoneal polymorphonuclear (PMN) granulocytes. In short-term in vivo experiments both forms administered intraperitoneally at 450 or 600 mg/kg inhibited the formation of carrageenin oedema in the rat paw. The long-term administration for 3 weeks of native and oxidized MTDQ-DS in doses of 450 mg/kg significantly inhibited the carrageenin-induced oedema formation, the zymosan-induced chemotaxis, and the luminol-dependent light production of rat peritoneal PMN granulocytes. The results suggest that the native and oxidized forms of MTDQ-DS have an antiinflammatory effect since they inhibit several events in the local inflammatory reaction.

Animals↗
PubMed1984

[Recurrence prevention of papillomas of the bladder using lycurim instillation].

Following surgical removal of papillary tumours of the bladder from 60 patients the authors performed short instillations of the bladder with LYCURIM. The histological picture revealed tumours in the stage O-A-B1 and grade I-II-III. In a control group (n = 129) 89 recidive cases were observed in 2 years. Out of the 60 patients of the LYCURIM group 19 (31.7%) were free of tumours for 2 years and 43 (71.7%) for 11/2 years. A transient cystitis which occurred with short application of the medicament was easily cured. Toxic effects on the blood picture were not observed.

Administration, Topical↗
PubMed1977

Separation of amino acids and peptides on non-polar stationary phases by high-performance liquid chromatography.

Microparticulate non-polar stationary phases, such as octadecyl-silica offer a rapid and efficient means for the separation of peptides and amino acids by high-performance liquid chromatography. Retention is attributed to hydrophobic interaction between the solutes and the hydrocarbonaceous functions covalently bound to the stationary phase surface. Consequently the species are eluted in the order of increasing hydrophobicity. Various peptide mixtures were analyzed by using gradient elution with increasing acetonitrile concentration in the eluent and monitoring the column effluent at 200 or 210 nm with an UV detector. The separation of angiotensins and enzymic digest of polypeptides illustrates the speed of the method which can be used to assay the purity of peptide hormones such as alpha-melanotropin and gramicidin or to analyze the composition of reaction mixtures involving peptides. The efficiency of the method is superior to that obtained on the conventionally used ion-exchanger columns, except for hydrophilic amino acids and peptides that are poorly retarded. Nevertheless, with a suitable ionic surfactant in the mobile phase, non-polar stationary phases can be used for the separation of these species as well.

Amino Acids↗
PubMed1977

Rapid separation of urinary acids by high-performance liquid chromatography.

Over a hundred acidic urinary constituents were separated within 30 min by using 5-micron octadecyl-silica columns and gradient elution with increasing acetonitrile concentration in dilute aqueous phosphoric acid solution at 70 degrees. The column effluent was monitored with a UV detector at 280 nm or with a fluorescence detector at 260 nm excitation and 340 nm emission wavelengths. The high sensitivity and speed of analysis, the excellent reproducibility and adequate resolution obtained suggest that this technique may be useful to obtain metabolic profiles in routine clinical work.

Carboxylic Acids↗