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I Mononen

Publications and source records attributed to I Mononen.

59 records · Page 4Linked to original sources
PubMed1982

Glycosylation of proteins in developing human brain.

The carbohydrate content of human brain glycoproteins was studied during development from the age of 12 fetal weeks to 8 postnatal months. The concentration of all the glycoprotein monosaccharides increased with age. The total amount of glycoprotein monosaccharides per lipid-free dry tissue increased by about 150% from the end of the third fetal month to the time of delivery. The increase leveled off around term, and only minor increase occurred after birth. The adult level was reached by the fifth postnatal month.

Adult↗
PubMed1977

Analysis of hexosaminitol-containing disaccharide alditols from rat brain glycoproteins and gangliosides as O-trimethylsilyl derivatives by gas chromatography mass spectrometry.

The O-trimethylsilyl derivatives of five reference disaccharide alditols composed of a N-acetylhexosaminitol substituted at C-3, C-4 or C-6 with a hexose moiety were studied by gas chromatography mass spectrometry. The data were used in the determination of the linkage in disaccharide alditols derived from rat brain glycoproteins and gangliosides. The O-glycosidically linked carbohydrate units of brain glycoproteins contained two oligosaccharides, alpha-galactosyl-(1 leads to 3)-N-acetylgalactosaminitol and beta-galactosyl-(1 leads to 3)-N-acetylgalactosaminitol, whereas only the latter was obtained from brain gangliosides after partial acid hydrolysis and reduction.

Animals↗
PubMedDate not supplied

Total and lipid-bound serum sialic acid in benign and malignant breast disease.

Elevation in the total sialic acid (TSA), TSA/total protein (TSA/TP) and lipid-bound sialic acid (LASA) concentration in serum occurs in breast cancer and we have studied the applicability of the assays in classification of undefined breast tumors. Sialic acid was determined by HPLC and the statistical evaluation included the receiver operating characteristic (ROC) and Youden's index analyses. In cancer patients, the serum LASA and TSA concentration was significantly higher (p < 0.05) than in patients with benign breast disease and all the markers were significantly higher (p < 0.0001) than in normal controls. All the markers had a low accuracy (AUCs < 0.75) in differentiating between breast cancer and benign breast disease and at the specificity level of 0.95 the corresponding sensitivities were 0.32 (TSA), 0.14 (TSA/TP) and 0.23 (LASA). The results indicate that both breast cancer and benign breast disease cause elevation of TSA, TSA/TP and LASA values in serum and do not provide reliable classification of undefined breast tumors.

Adolescent↗
PubMedDate not supplied

Total and lipid-bound serum sialic acid in children with malignancy or infections.

Increased levels of sialic acid-containing glycoconjugates in serum have been observed in malignancies. In this study, significantly elevated serum concentrations of total sialic acid (TSA), TSA/total protein (TSA/TP) and lipid-bound sialic acid (LASA) were observed at diagnosis of children with various malignancies compared to healthy children. Serum TSA and TSA/TP were superior to LASA in differentiating solid tumors (p < 0.005) and leukemias (p < 0.05) from normal controls. In neuroblastoma and yolk sac tumors, a descending trend in TSA and TSA/TP was noted after successful treatment of the malignancy. Children with simultaneous malignancy and infection had significantly higher serum TSA (p < 0.05) and TSA/TP (p < 0.01) levels compared to patients with infectious diseases only, the corresponding areas under the curve were 0.83 and 0.88. The measurement of serum TSA and TSA/TP could be useful adjuncts in exclusion, diagnosis and follow-up of malignancies in children.

Adolescent↗