PubMed Health⌕ Search

Biomedical subjects

I Moro

Publications and source records attributed to I Moro.

At least 91 records · Page 5Linked to original sources
PubMed1987

Ultrastructural and immunocytochemical characterization of polymorphonuclear leukocytes from gingival crevice in man.

Polymorphonuclear leukocytes from the gingival crevicular fluid (CF-PMNs) of patients with generalized severe periodontitis were examined using electron microscopy and immunocytochemical techniques. CF-PMNs were found to contain numerous phagocytic vacuoles. This suggests that CF-PMNs actively phagocytized various substances from the environment. Immunocytochemical staining with FITC-conjugated IgG, IgM, and IgA reagents and TRITC-conjugated C3 reagent was applied to CF-PMNs as well as peripheral blood PMNs incubated with cell-free crevicular fluid. The cytoplasm of PMNs exhibited numerous granular foci of immunofluorescence. This finding suggests that these proteins were acquired from the environment by PMNs. The coincidental appearances of immunoglobulins and C3 in a single cell were considered to be immune complexes phagocytized by CF-PMNs in generalized severe periodontitis.

Adult↗
PubMed1987

Derivation of a murine monoclonal antibody useful for immunohistochemical diagnosis of human adenoid cystic carcinoma and stomach cancer.

A monoclonal antibody, NCC-SG-007, was raised with a formalin-fixed adenoid cystic carcinoma of the salivary gland as the immunogen. The reactivity of the antibody was tested on paraffin sections using the avidin-biotin-peroxidase complex (ABC) method. NCC-SG-007 reacted with 36% of the adenoid cystic carcinomas, 42% of the pleomorphic adenomas and 14% of the mucoepidermoid tumors of the salivary gland which were tested. The antibody also reacted with some normal tissues and various other tumors. Surprisingly, NCC-SG-007 showed a reactivity as high as 94% with gastric carcinomas. The antigenic determinant was revealed to be a carbohydrate chain with no terminal sialic acid, the molecular weight of which was estimated to be 1.5 X 10(6) daltons. This antigen differed from other previously reported gastrointestinal cancer-associated antigens. The antibody should be useful for studying adenoid cystic carcinomas of the salivary gland and gastric cancers.

Animals↗
PubMed1986

Selective phagocytosis of gram-positive bacteria and interleukin 1-like factor production by a subpopulation of large granular lymphocytes.

There has been a consensus that a large granular lymphocyte (LGL) population with natural killer (NK) function is nonadherent and nonphagocytic. However, a significant proportion of the nonadherent cells purified by the two-step depletion of adherent cells with a plastic surface and nylon wool columns engulfed Sta. aureus into their cytoplasm. These cells were morphologically identified as LGL in light and electron microscopies. Two-color immunofluorescence tests, furthermore, demonstrated that Leu-11+ LGL, Leu-11+7-, and Leu-11+7+, but not Leu-11-7+, phagocytosed Sta. aureus. Among the particles tested here, only Gram(+) bacteria were preferentially phagocytosed, whereas Gram(-) bacteria, other large-sized microbes (e.g., baker's yeast and Candida albicans), latex, silica, and carbonyl iron were not. LGL exhibited a substantial level of bactericidal activity against Sta. aureus, although the level was one third of that mediated by monocytes. When Gram(+) bacteria were incubated with nonadherent cells for 18 hr, significant amounts of interleukin 1 (IL 1)-like factors (or IL 1 itself) as well as interferon were detected in the supernatants. On the other hand, this incubation did not induce interleukin 2 (IL 2). The IL 1-like factor producer cells were demonstrated to be the low-density lymphocytes on Percoll separation and to have the Leu-11+ phenotype. The phagocytosis was suggested to be an important stimulus in producing IL 1-like factors from LGL. Thus, the treatment of cells with cytochalasin B, a microfilament disrupting agent, completely abrogated both phagocytosis and IL 1-like factor production. Some cell wall components of Gram(+) bacteria might be important to a recognition process of the phagocytosis, since the protoplasts of Sta. aureus, when prepared by the treatment of bacteria with lysostaphin, were no longer phagocytosed by LGL. The present results therefore identify an additional unique characteristic similar to, but not identical with, the myelomonocytic nature of Leu-11+ LGL.

Adult↗
PubMed1986

Inhibition of natural killer cell activity by IgA.

The in vitro effect of IgA on natural killer (NK) activities of human peripheral blood mononuclear cells was investigated. Purified myeloma polymeric IgA2 (pIgA2) and secretory IgA (S-IgA) from human colostrum inhibited NK activity, while myeloma polymeric IgA1 (pIgA1), monomeric IgA1 (mIgA1), IgG, and IgM were ineffective. Inhibition was proportional to the concentration of pIgA2 (0.125-1 mg/ml) and was observed after as little as 1 hr of incubation at various effector to K562 target cell ratios. pIgA2 and S-IgA also inhibited NK activity of NK cell-enriched lymphoid cells and gamma-interferon-treated effector cells, but did not interfere with effector-target cell binding. The inhibitory effect was slightly diminished after 24 hr culture in pIgA2-free medium. Inhibition of cytotoxicity was not due to direct toxicity on lymphoid cells by IgA because PBL treated with pokeweed mitogen in the presence of pIgA2 or S-IgA differentiated into immunoglobulin-producing cells. Viability after 24 hr of preculture with pIgA2 and S-IgA was comparable to that of untreated control cells. Morphological examination of effector cells cultured with pIgA2 or S-IgA showed a decrease in the number of granules, and the formation of cytoplasmic vacuoles. These morphological changes appeared to coincide with the depressed cytotoxicity of NK cells. The results demonstrate that purified pIgA2 and S-IgA have significant immunomodulatory effects on human NK activity.

Binding, Competitive↗
PubMed1986

[Immunohistochemical study on human colostral cells].

Colostrum contains a large number of cells and humoral defence factors that play an important role in the protection of neonates. While colostral cells have been characterized by immunohistochemical criteria, the function of these cells has been poorly understood. In this study, localization of immunoglobulins and other components in human colostral cells was examined by an immunofluorescence technique. The results obtained were as follows: Colostral cells reactive with polyvalent anti-human immunoglobulins were neutrophils and macrophages with a granular staining pattern. The predominant immunoglobulin in colostral cells was IgA, followed by IgM. A small amount of IgG was also found in these cells. The distribution of SC was similar to that of IgA and J-chain, suggesting the presence of secretory IgA in colostral cells. Coincidence of kappa chain and lambda chain in the same granules of neutrophils and macrophages suggests that these immunoglobulins were phagocytosed by neutrophils and macrophages. This conclusion was supported the results of the experiment which showed that incubation of peripheral blood neutrophils and monocytes with cell free colostrum resulted in the appearance of immunoglobulins in granules.

Colostrum↗
PubMed1985

Natural killer cells in human colostrum.

The presence of natural killer cells in human colostrum was disclosed with the use of a fluorochrome-labeled monoclonal antibody HNK-1 (Leu-7) that recognizes cells with natural killer and killer activity. Approximately 0.5% of total colostral cells were stained with this reagent. These cells were separated by the fluorescence-activated cell sorter and examined for their morphology by electron microscopy and for their cytotoxic activity against 51Cr-labeled K562 target cells. Two morphological types of natural killer cells were observed in colostrum: the first was represented by large cells with numerous vacuoles but without dense cytoplasmic granules; the second type, which occurred with lower frequency, resembled the large granular lymphocytes associated with natural killer activity in peripheral blood. The HNK-1-positive cells from colostrum displayed low cytotoxic activity against K562 target cells. Incubation of HNK-1-positive cells from peripheral blood with cell-free colostrum resulted in a dose-dependent inhibition of the cytotoxic activity. The functional changes were accompanied by morphological alterations which included degranulation and the formation of numerous vacuoles. The variances in the cytotoxic activity of peripheral blood HNK-1-positive cells suspended in different dilutions of colostrum suggest that this fluid contains humoral factors which modify morphology and function depending on their concentrations.

Blood Cells↗
PubMed1984

Immunohistochemical distribution of immunoglobulins, lactoferrin, and lysozyme in human minor salivary glands.

The immunofluorescence technique was used to examine the distribution of immunoglobulin A and its subclasses, secretory component (SC), J chain, lactoferrin and lysozyme in labial and lingual (von Ebner's) glands. IgA-containing plasma cells were found in the connective tissue around intercalated or intralobular ducts and a few were noted around acini of both glands. IgA was detected in the apical cytoplasm of intercalated and intralobular duct cells and in acini of von Ebner's glands and in demilunes of labial glands. Most IgA-containing cells also stained for J chain. The ratio of IgA1:IgA2-containing cells was approximately equal in von Ebner's and labial glands. Cytoplasmic and surface membrane-related staining for SC was detected in epithelial cells of the intercalated and intralobular ducts in both glands, in the serous acini of von Ebner's gland, and in the demilunes of labial glands. Lactoferrin was found in serous acini, demilunes, intercalated and intralobular ducts. Lysozyme was found in acinar and intercalated ducts, but was rarely seen in intralobular ducts. These results disclose the presence of cells (plasma cells and epithelial cells) and their products (IgA and secretory component) that indicate the local production of secretory IgA in minor salivary glands.

Fluorescent Antibody Technique↗
PubMed1984

Immunohistochemical characterization of functional markers in human minor salivary gland tumors.

The distribution of carcinoembryonic antigen (CEA), secretory component (SC), immunoglobulin A (IgA), immunoglobulin M (IgM), J-chain, and lysozyme in tumors of minor salivary glands was investigated using an immunoperoxidase method. Although CEA was demonstrated in both benign and malignant tumors, its distribution was relatively more common and with increased staining intensity in malignant tissues. In pleomorphic adenomas, the distribution of SC was similar to that of IgA and J-chain, suggesting the presence of secretory IgA in the epithelial cells. However, some neoplastic epithelial cells contained SC but not IgA and J-chain. No IgM was detected in such cells. Lysozyme could be demonstrated only in pleomorphic adenomas. Mucoepidermoid tumors and adenoidcystic carcinomas were negative for lysozyme. These findings suggest that some neoplastic ductal epithelial cells of pleomorphic adenomas retain functional characteristics of normal epithelial cells.

Adenoma, Pleomorphic↗
PubMed1983

Localization of IgA and IgM in human colostral elements using immunoelectron microscopy.

In addition to the free form, IgA is associated with cellular and noncellular elements present in human colostrum. To resolve the existing controversy as to the cell type(s) containing IgA, we used immunoelectron microscopy with horseradish peroxidase-labeled F(ab')2 or Fab' fragments of anti-IgA or anti-IgM to determine the distribution of these immunoglobulins in colostral elements. IgA and IgM were localized in phagocytic vacuoles of polymorphonuclear leukocytes and macrophages in the vicinity of the cell membrane. In neutrophilic leukocytes, both immunoglobulins were occasionally found in phagocytic vacuoles distributed throughout the cytoplasm. Although the in vitro phagocytic activity of colostral cells was low, they retained the ability to ingest colloidal gold particles which were subsequently localized in phagocytic vacuoles that also contained IgA or IgM. IgA and IgM were not detected in lymphocytes, and plasma cells were not found in human colostrum. Numerous noncellular colostral globules of various shapes and sizes also contained IgA and IgM. These observations indicate that IgA and IgM were acquired by phagocytic cells and noncellular globules and were not actively synthesized by lymphoid cells present in human colostrum.

Colostrum↗
PubMed1982

The eosinophilic and amyloid-like materials in adenomatoid odontogenic tumor.

This paper is concerned with the relationship between eosinophilic material (EM) and amyloid-like material and adenomatoid odontogenic tumors. In duct-like structures between opposing rows of tall columnar cells, EM did not stain for amyloid. Under electron microscopy, EM was composed of fibrillar and granular materials, and the fibrillar material was not amyloid. Two different kinds of EM were found in solid cell masses. Lesions from cases 2, 3, 4 and part of case 1 contained small droplet-shaped EM and these EM did not stain for amyloid. Case 1 also contained EM that stained positively for amyloid. The structure of amyloid positive EM resembled developing enamel of human tooth germs. This material was tubular and finely granular. The tubular material resembled enamel matrix fibers rather then amyloid and the fine granular material was stippled. The cells surrounding EM appeared similar to ameloblasts between secretory and maturation stages.

Adolescent↗