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Biomedical subjects

I N Shokolenko

Publications and source records attributed to I N Shokolenko.

6 recordsLinked to original sources
PubMed1995

Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector construction and in vitro deletion/insertion mutagenesis.

Several antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector construction include the aacC1, aadA+, bla, cat, nptII and tet gene cassettes, and also the omega-Gm, omega-Sm, omega-Ap, omega-Cm, omega-Km and omega-Tc elements. Both cassettes and elements are flanked by pBluescriptII plasmid multiple cloning sites (MCS) duplicated in inverted (symmetric MCS) or direct (tandem MCS) orientation. Genes that were modified in order to remove sites for the most common restriction endonucleases from their coding regions (except aacC1 and aadA+) were used for cassette and omega-element construction.

Anti-Bacterial Agents↗
PubMed1995

Mini-Tn10 transposon derivatives for insertion mutagenesis and gene delivery into the chromosome of gram-negative bacteria.

Four mini-Tn10 derivatives bearing the nptII, cat, aacC1 and tet genes along with MCS of the pBluescriptII plasmid were constructed. These derivatives are embedded into the gamma-ori R6K-based suicide plasmid pBSL177, which can be mobilized into a broad range of hosts by the RP4 plasmid. The relatively small size and the presence of the mutant ATS Tn10 transposase gene, fused to Ptac promoter, as well as the versatile MCS of the pBluescriptII plasmid make these vectors a reliable tool for insertion mutagenesis and chromosomal insertion of the cloned DNA fragments.

Chromosomes, Bacterial↗
PubMed1995

New mini-Tn5 derivatives for insertion mutagenesis and genetic engineering in gram-negative bacteria.

Five mini-Tn5 derivatives encoding resistance to Km, Cm, Gm, Tc, and Sm, coupled with the polylinker of the pBluescriptII plasmid, were constructed. These derivatives are carried by an ampicillin-resistant plasmid that has a conditional origin of replication from plasmid R6K and origin of conjugal transfer from the broad host range plasmid RP4. The new vectors are smaller than those previously described and possess numerous unique restriction sites inside the minitransposons for gene cloning in addition to SfiI and NotI sites found in their predecessors.

DNA Nucleotidyltransferases↗
PubMed1991

[Cycle dependent expression of the gene coding for B2mRNA].

The relative contents of individual tissue specific RNA B2mRNAx was studied in the population of nuclear and poly(A)+ cytoplasmic RNA from the livers of intact, falsely operated and having suffered the partial liver resection rats. Dot-hybridization technique was used to study this transcript containing the transcribed copy of the B2 repetitive genetic element of rats. The expression of the gene coding for B2mRNAx takes place at the lower level in the liver induced to proliferation as compared with the one in the liver of intact animals. It is changed reproducibly in antiphase with the c-fos RNA and with major inclinations at the moments of cellular phases switch.

Animals↗