Biomedical subjects
Publications and source records attributed to I Nada.
A tree model is presented in order to illustrate the hypotheses that earlier stem cells will respond differently than later progenitors to the same stimuli. Experimental data and decision analysis tools are shown to demonstrate the concept.
The effects of recombinant alpha, beta and gamma interferons (IFN), alone or in combinations were studied in a case of essential thrombocythemia, using the mixed colony formation assay. This assay allows growth of multipotent (CFU-Mix), and unipotent granulocytic-macrophage (CFU-GM), erythroid (BFU-E) and megakaryocytic (CFU-Meg) progenitors. The bone marrow precursors were cultured in the presence of each type of IFN at 100 U/ml and all possible combinations performed. The results were analysed using the Shapley formula, a game theoretic approach. It is concluded that IFN alpha would be the best candidate for reducing megakaryocytic progenitors while growth of other hematopoietic precursors would be stimulated. These types of analytical biological experiments controlled by using six permutations and calculation of the Shapley value for the three types of IFNs are suggested as a fair approach for a rational choice.
Explore the source record for details and available documents.