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I Nishida

Publications and source records attributed to I Nishida.

42 records · Page 3Linked to original sources
PubMed1986

Effect of thiolactomycin on the individual enzymes of the fatty acid synthase system in Escherichia coli.

Thiolactomycin, an antibiotic with the structure of (4S)-(2E,5E)-2,4,6-trimethyl-3-hydroxy-2,5,7-octatriene-4-++ +thiolide, selectively inhibits type II fatty acid synthases. The mode of the thiolactomycin action on the fatty acid synthase system of Escherichia coli was investigated. Of the six individual enzymes of the fatty acid synthase system, [acyl-carrier-protein] (ACP) acetyltransferase and 3-oxoacyl-ACP synthase were inhibited by thiolactomycin. On the other hand, the other enzymes were not affected by this antibiotic. The thiolactomycin inhibition of the fatty acid synthase system was reversible. As to ACP acetyltransferase, the inhibition was competitive with respect to ACP and uncompetitive with respect to acetyl-CoA. As to 3-oxoacyl-ACP synthase, the inhibition was competitive with respect to malonyl-ACP and noncompetitive with respect to acetyl-ACP. The thiolactomycin action on the fatty acid synthase system was compared with that of cerulenin.

3-Oxoacyl-(Acyl-Carrier-Protein) Synthase↗
PubMedDate not supplied

Attenuation of aminoglycoside ototoxicity by glutathione.

Aminoglycoside antibiotics are commonly used for the treatment of serious gram-negative infections despite a high incidence of associated ototoxicity. Attempts to elucidate the mechanisms of toxicity or prevent the adverse effects have previously been unsuccessful. Recently, the damaging effects of aminoglycosides on the inner ear have been shown to be caused by a metabolite of the drug, implying an enzymatic conversion of the parent compound. Glutathione has been suggested to be closely related to the detoxification mechanisms of this metabolite. This study revealed the possible attenuation of aminoglycoside ototoxicity by glutathione. Guinea pigs were given amikacin alone or amikacin with prior intramuscular injection of glutathione. The pretreatment of glutathione significantly reduced the damage of outer hair cells of the organ of Corti. This may indicate that glutathione reduces the aminoglycoside ototoxicity and can be favorably applied in clinical use.

Amikacin↗